Articles

Hypoxia destroys the microstructure of microtubules and causes dysfunction of endothelial cells via the PI3K/Stathmin1 pathway

Endothelial cells (EC) are sensitive to changes in the microenvironment, including hypoxia and ischemia. Disruption of the microtubular network has been reported in cases of ischemia. However, the signaling pa...

Authors: Huaming Cao, Dongsheng Yu, Xueyun Yan, Bing Wang, Zhiming Yu, Yu Song and Liang Sheng

Exosomes: biogenesis, biologic function and clinical potential

Exosomes are nano-sized biovesicles released into surrounding body fluids upon fusion of multivesicular bodies and the plasma membrane. They were shown to carry cell-specific cargos of proteins, lipids, and ge...

Authors: Yuan Zhang, Yunfeng Liu, Haiying Liu and Wai Ho Tang

Comparison of osteogenic differentiation capacity in mesenchymal stem cells derived from human amniotic membrane (AM), umbilical cord (UC), chorionic membrane (CM), and decidua (DC)

Mesenchymal stem cells (MSCs) have been extensively explored as a promising therapeutic agent in the field of bone tissue engineering due to their osteogenic differentiation ability. In this study, the osteoge...

Authors: Chongyang Shen, Chuan Yang, Shijun Xu and Hai Zhao

Regional methylome profiling reveals dynamic epigenetic heterogeneity and convergent hypomethylation of stem cell quiescence-associated genes in breast cancer following neoadjuvant chemotherapy

Neoadjuvant chemotherapy (NAC) induces a pathological complete response (pCR) in ~ 30% of patients with breast cancer. However, aberrant DNA methylation alterations are frequent events during breast cancer pro...

Authors: Yumei Luo, Juan Huang, Yi Tang, Xitu Luo, Lingxia Ge, Xiujie Sheng, Xiaofang Sun, Yaoyong Chen and Detu Zhu

Growth inhibition and apoptosis in colorectal cancer cells induced by Vitamin D-Nanoemulsion (NVD): involvement of Wnt/β-catenin and other signal transduction pathways

More than the two decades, the question of whether vitamin D has a role in cancer frequency, development, and death has been premeditated in detail. Colorectal, breast, and prostate cancers have been a scrupul...

Authors: Suhail Razak, Tayyaba Afsar, Ali Almajwal, Iftikhar Alam and Sarwat Jahan

Long non-coding RNA BLACAT1 promotes breast cancer cell proliferation and metastasis by miR-150-5p/CCR2

Breast cancer was dangerous to women health. A growing number of evidences indicate that long non-coding RNAs (lncRNAs) have many functions in the development and progression of breast cancer and may serve as ...

Authors: Xiaopeng Hu, Yun Liu, Yaying Du, Teng Cheng and Wenfei Xia

Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression

Psychological and physical stress can either enhance or suppress immune functions depending on a variety of factors such as duration and severity of stressful situation. Chronic stress exerts a significantly s...

Authors: Anna Qin, Ting Zhong, Huajiao Zou, Xiaoya Wan, Bifeng Yao, Xinbin Zheng and Deling Yin

Scutellarin protects human retinal pigment epithelial cells against hydrogen peroxide (H₂O₂)-induced oxidative damage

Proliferative vitreoretinopathy (PVR) is a severe blinding complication of retinal detachment surgery. Increasing evidence demonstrate that PVR is associated with oxidative stress. Scutellarin is a natural fla...

Authors: Xin Hu, Xiaofang Wu, Bo Zhao and Yongyi Wang

Long non-coding RNA ANRIL-mediated inflammation response is involved in protective effect of rhein in uric acid nephropathy rats

The aim of this study was to investigate the role of long non-coding RNAs (LncRNAs) antisense non-coding RNA in the INK4 locus (ANRIL) in anti-inflammation of rhein in uric acid nephropathy (UAN) rats.

Authors: Jiacai Hu, Daochun Wang, Hao Wu, Zhijie Yang, Na Yang and Junjun Dong

IL-33 ameliorates experimental colitis involving regulation of autophagy of macrophages in mice

Previously, we have demonstrated that IL-33 administration protecting TNBS-induced experimental colitis is associated with facilitation of Th2/Tregs responses in mice. However, whether IL-33 regulates autophag...

Authors: Zhongyan Wang, Lifeng Shi, Shuyao Hua, Chang Qi and Min Fang

Transmembrane protein 108 involves in adult neurogenesis in the hippocampal dentate gyrus

Transmembrane protein 108 (Tmem108) is a risk gene of psychiatric diseases including schizophrenia, bipolar disorder and major depression disorder. However, the pathophysiological mechanisms of Tmem108 are lar...

Authors: Zheng Yu, Dong Lin, Yanzi Zhong, Bin Luo, Shengsheng Liu, Erkang Fei, Xinsheng Lai, Suqi Zou and Shunqi Wang

Dynamics expression of DmFKBP12/Calstabin during embryonic early development of Drosophila melanogaster

Calcium signaling are conserved from invertebrates to vertebrates and plays critical roles in many molecular mechanisms of embryogenesis and postnatal development. As a critical component of the signaling path...

Authors: Rui Feng, Xin Zhou, Wei Zhang, Tao Pu, Yuting Sun, Rong Yang, Dan Wang, Xiaofei Zhang, Yingfeng Gao, Zhenlu Cai, Yu Liang, Qiuxia Yu, Yajun Wu, Xinjuan Lei, Zhijia Liang, Odell Jones…

A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration

Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes...

Authors: Yiming Wang, Song Chen, Zuoqin Yan and Ming Pei

XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC

The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells.

Authors: Zhihua Xiao, Yijun Wang and Hao Ding

Cell type-specific function of TRAF2 and TRAF3 in regulating type I IFN induction

TRAF3 is known as a central mediator of type I interferon (IFN) induction by various pattern recognition receptors, but the in vivo function of TRAF3 in host defense against viral infection is poorly defined d...

Authors: Xiaoping Xie, Jin Jin, Lele Zhu, Zuliang Jie, Yanchuan Li, Baoyu Zhao, Xuhong Cheng, Pingwei Li and Shao-Cong Sun

1-Palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (PLAG) attenuates gemcitabine-induced neutrophil extravasation

Cancer patients treated with chemotherapy often experience a rapid decline of blood neutrophils, a dose-limiting side effect called chemotherapy-induced neutropenia. This complication brings about dose reducti...

Authors: Jinseon Jeong, Yong-Jae Kim, Do Young Lee, Byoung-Gon Moon, Ki-Young Sohn, Sun Young Yoon and Jae Wha Kim

STAT3 enhances the constitutive activity of AGC kinases in melanoma by transactivating PDK1

The PI3K/Akt and the STAT3 pathways are functionally associated in many tumor types. Both in vitro and in vivo studies have revealed that either biochemical or genetic manipulation of the STAT3 pathway activit...

Authors: María Elisa Picco, María Victoria Castro, María Josefina Quezada, Gastón Barbero, María Belén Villanueva, Natalia Brenda Fernández, Hyungsoo Kim and Pablo Lopez-Bergami

The first structure of HIV-1 gp120 with CD4 and CCR5 receptors

Shaik et al. recently published online the cryo-electron microscopy structure of HIV-1 gp120 in complex with CD4 and CCR5 receptors. This is the first structure of the ternary HIV-1 gp120/CD4/CCR5 complex. Thi...

Authors: Yongjun Guan

Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells

To determine whether and how exosomes from human umbilical vein endothelial cells (HUVEC-Exos) regulates vascular smooth muscle cells (VSMCs) calcification/senescence in high glucose condition.

Authors: Shuang Li, Jun-Kun Zhan, Yan-Jiao Wang, Xiao Lin, Jia-Yu Zhong, Yi Wang, Pan Tan, Jie-Yu He, Xing-Jun Cui, Yi-Yin Chen, Wu Huang and You-Shuo Liu

Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro

Sialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialy...

Authors: Sebastian Strubl, Uwe Schubert, Andrea Kühnle, Alexander Rebl, Negah Ahmadvand, Silvia Fischer, Klaus T. Preissner and Sebastian P. Galuska

The roles of lncRNA in hepatic fibrosis

Increasing evidence indicates that long non-coding RNAs (lncRNAs) regulate gene or protein expression; however, their function in the progression of hepatic fibrosis remains unclear. Hepatic fibrosis is a cont...

Authors: Hu Peng, Lin-Yan Wan, Jia-Jie Liang, Yan-Qiong Zhang, Wen-Bing Ai and Jiang-Feng Wu

TIPE2 acts as a biomarker for GIST risk category and suppresses the viability and invasiveness of GIST cells

Evaluating the risk category of gastrointestinal stromal tumors (GISTs) is crucial for predicting prognosis and choosing treatment strategies, and tumor metastasis usually represent poor prognosis. Tumor necro...

Authors: Zequn Li, Wei Zhang, Yi Li, Shougen Cao, Shanglong Liu, Liang Ning, Xuelong Jiao, Zimin Liu, Xiaoming Xing, Yujun Li and Yanbing Zhou

TUG1 confers cisplatin resistance in esophageal squamous cell carcinoma by epigenetically suppressing PDCD4 expression via EZH2

Increasing evidence has suggested the involvement of long non-coding RNA taurine upregulated gene 1 (TUG1) in chemoresistance of cancer treatment. However, its function and molecular mechanisms in esophageal s...

Authors: Caihui Xu, Yinmou Guo, Haiyan Liu, Gongbin Chen, Yanju Yan and Teng Liu

(+)-JQ1 attenuated LPS-induced microglial inflammation via MAPK/NFκB signaling

Microglia activation is a crucial event in neurodegenerative disease. The depression of microglial inflammatory response is considered a promising therapeutic strategy. NFκB signaling, including IKK/IκB phosph...

Authors: Huanhuan Wang, Wenhai Huang, Meihao Liang, Yingying Shi, Chixiao Zhang, Qin Li, Meng Liu, Yikai Shou, Hongping Yin, Xiaozheng Zhu, Xiaoyan Sun, Yu Hu and Zhengrong Shen

Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing

Artificial nuclease-dependent DNA cleavage systems (zinc-finger nuclease, ZFN; transcription activator like effectors, TALENs) and exogenous nucleic acid defense systems (CRISPR/Cas) have been used in the new ...

Authors: Yuyi Tang and Yan Fu

Circ_0027599/PHDLA1 suppresses gastric cancer progression by sponging miR-101-3p.1

Pleckstrin homology-like domain family A member 1 (PHLDA1) is a tumor suppressor gene in gastric cancer, but its role regulated by circular RNAs (circRNAs) is not known. CircRNAs are important regulators in ca...

Authors: Liang Wang, Jingyan Shen and Yushan Jiang

CITED1 promotes proliferation of papillary thyroid cancer cells via the regulation of p21 and p27

It has been reported that CBP/p300-Interacting Transactivator with glutamic acid [E]/aspartic acid [D]-rich C-terminal domain 1 (CITED1) is overexpressed in papillary thyroid cancer (PTC). However, the functio...

Authors: Hai Li, Hongyu Guan, Yan Guo, Weiwei Liang, Liehua Liu, Xiaoying He, Weijian Ke, Xiaopei Cao, Haipeng Xiao and Yanbing Li

Essential roles of mitochondrial and heme function in lung cancer bioenergetics and tumorigenesis

Contrary to Warburg’s hypothesis, mitochondrial oxidative phosphorylation (OXPHOS) contributes significantly to fueling cancer cells. Several recent studies have demonstrated that radiotherapy-resistant and ch...

Authors: Sarada Preeta Kalainayakan, Keely E. FitzGerald, Purna Chaitanya Konduri, Chantal Vidal and Li Zhang

MicroRNA-29a-3p enhances dental implant osseointegration of hyperlipidemic rats via suppressing dishevelled 2 and frizzled 4

Fine osseointegration is the basis of long-term survival of implant. In our previous study, we observed a strong correlation between hyperlipidemia and compromised osseointegration. MicroRNA-29a-3p (miR-29a-3p...

Authors: Fei Liu, Zhifeng Wang, Fangfang Liu, Jinzhao Xu, Qibo Liu, Kaifeng Yin and Jing Lan

A role of Pumilio 1 in mammalian oocyte maturation and maternal phase of embryogenesis

RNA binding proteins play a pivotal role during the oocyte-to-embryo transition and maternal phase of embryogenesis in invertebrates, but their function in these processes in mammalian systems remain largely u...

Authors: Winifred Mak, Jing Xia, Ee-Chun Cheng, Katie Lowther and Haifan Lin

EF-hand domain containing 2 (Efhc2) is crucial for distal segmentation of pronephros in zebrafish

The blood filtering organ in zebrafish embryos is the pronephros, which consists of two functional nephrons. Segmentation of a nephron into different domains is essential for its function and is well conserved...

Authors: Praveen Barrodia, Chinmoy Patra and Rajeeb K. Swain

Phagocytosis influences the intracellular survival of Mycobacterium smegmatis via the endoplasmic reticulum stress response

Mycobacterium smegmatis, a rapidly growing non-tuberculosis mycobacterium, is a good model for studying the pathogenesis of tuberculosis because of its genetic similarity to Mycobacterium tuberculosis (Mtb). Macr...

Authors: Seon-Hwa Kim, Soo-Na Cho, Yun-Ji Lim, Ji-Ae Choi, Junghwan Lee, Dam Go and Chang-Hwa Song

The balance of two opposing factors Mad and Myc regulates cell fate during tissue remodeling

Cell proliferation and differentiation are two distinct yet coupled processes in development in diverse organisms. Understanding the molecular mechanisms that regulate this process is a central theme in develo...

Authors: Morihiro Okada and Yun-Bo Shi

Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer

We investigated the inhibitory effect of pantoprazole on signal transducer and activator of transcription 3 (STAT3) activity and invasiveness of gastric adenocarcinoma cells, and the role of SH2-containing pro...

Authors: Jin Sung Koh, Moon Kyung Joo, Jong-Jae Park, Hyo Soon Yoo, Byung Il Choi, Beom Jae Lee, Hoon Jai Chun and Sang Woo Lee

Metabolic alterations induced by attenuated Zika virus in glioblastoma cells

Zika virus (ZIKV) has recently become a major matter of concern since its association with microcephaly cases in newborns was determined. From then on, ZIKV has been untiringly studied, and several scientific ...

Authors: Mohamed Ziad Dabaja, Estela de Oliveira Lima, Diogo Noin de Oliveira, Tatiane Melina Guerreiro, Carlos Fernando Odir Rodrigues Melo, Karen Noda Morishita, Marcelo Lancellotti, Ana Lucia Tasca Gois Ruiz, Gisele Goulart, Diego Andreazzi Duarte and Rodrigo Ramos Catharino

TIPE2 acts as a biomarker for tumor aggressiveness and suppresses cell invasiveness in papillary thyroid cancer (PTC)

Tumor necrosis factor (TNF)-alpha-induced protein 8-like 2 (TIPE2 or TNFAIP8L2) is a newly described negative immune regulator and is closely associated with various tumors. However, the expression and roles o...

Authors: Wenyu Jia, Zequn Li, Junyu Chen, Lei Sun, Chuanqian Liu, Shaping Wang, Jingwei Chi, Jun Niu and Hong Lai

All-trans retinoic-acid inhibits heterodimeric bone morphogenetic protein 2/7-stimulated osteoclastogenesis, and resorption activity

Bone regenerative heterodimeric bone morphogenetic protein 2/7 (BMP2/7) enhances but all-trans retinoic acid (ATRA) inhibits osteoclastogenesis. However, the effect of ATRA on physiological and/or BMP2/7-induc...

Authors: Wenjuan Bi, Yi Liu, Jing Guo, Zhen Lin, Jinsong Liu, Miao Zhou, Daniel Wismeijer, Janak L. Pathak and Gang Wu

Proteomic characterization of endogenous substrates of mammalian ubiquitin ligase Hrd1

Endoplasmic reticulum (ER)-associated degradation (ERAD) regulates protein homeostasis in the secretory pathway by targeting misfolded or unassembled proteins for degradation by the proteasome. Hrd1 is a conse...

Authors: Yilin Ye, Suk-Hwan Baek, Yihong Ye and Ting Zhang

Dysregulation of the calcium handling protein, CCDC47, is associated with diabetic cardiomyopathy

Diabetes mellitus is associated with an increased risk in diabetic cardiomyopathy (DCM) that is distinctly not attributed to co-morbidities with other vasculature diseases. To date, while dysregulation of calc...

Authors: Khampaseuth Thapa, Kai Connie Wu, Aishwarya Sarma, Eric M. Grund, Angela Szeto, Armando J. Mendez, Stephane Gesta, Vivek K. Vishnudas, Niven R. Narain and Rangaprasad Sarangarajan

The 2017 Ming K. Jeang award for excellence in Cell & Bioscience

Two research groups led by Dr. Shui Qing Ye of University of Missouri Kansas City School of Medicine, Kansas City, USA and Dr. Dihua Yu of University of Texas MD Anderson Cancer Center, Houston, USA, respectiv...

Authors: Yun-Bo Shi

Role of MCM2–7 protein phosphorylation in human cancer cells

A heterohexameric complex composed of minichromosome maintenance protein 2–7 (MCM2–7), which acts as a key replicative enzyme in eukaryotes, is crucial for initiating DNA synthesis only once per cell cycle. Th...

Authors: Liangru Fei and Hongtao Xu

Long non-coding RNA CASC15 promotes melanoma progression by epigenetically regulating PDCD4

Long non-coding RNAs (LncRNAs) have been identified as critical regulators in a variety of cancer types. Cancer susceptibility candidate 15 (CASC15), a lncRNA located at chromosome 6p22.3, has been discovered ...

Authors: Yakun Yin, Bin Zhao, Dongqin Li and Guangwen Yin

Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington’s disease

Iron accumulation in basal ganglia accompanies neuronal loss in Huntington’s disease (HD) patients and mouse disease models. Disruption of HD brain iron homeostasis occurs before the onset of clinical signs. T...

Authors: Li Niu, Cuifang Ye, Yun Sun, Ting Peng, Shiming Yang, Weixi Wang and He Li

NICD-mediated notch transduction regulates the different fate of chicken primordial germ cells and spermatogonial stem cells

Notch signaling is mainly regulated by Notch1 during development of chicken germ stem cells; however, the molecular mechanisms that contribute to generation of these germ stem cells have not been thoroughly in...

Authors: Qisheng Zuo, Chen Zhang, Kai Jin, Jin Jing, Changhua Sun, Mahmoud F. Ahmed, Jiuzhou Song, Yani Zhang, Guohong Chen and Bichun Li

Calcium signaling: an underlying link between cardiac disease and carcinogenesis

Authors: Xuehong Xu, Steven P. Balk, William B. Isaacs and Jianjie Ma

Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response

This study investigated the role of HSP70 in modulating intestinal γδ T cells’ Th17 response in Trichinella spiralis-induced PI-IBS mice model.

Authors: Zhoutao He, Xiaoning Sun, Zhichao Ma, Jiao Fu, Baili Huang, Fujin Liu, Yi Chen, Taozhi Deng, Xiangyang Han, Deming Sun and Cheng Lan

A superior bright NIR luminescent nanoparticle preparation and indicating calcium signaling detection in cells and small animals

Near-field fluorescence (NFF) effects were employed to develop a novel near-infrared (NIR) luminescent nanoparticle (LNP) with superior brightness. The LNP is used as imaging contrast agent for cellular and sm...

Authors: Jian Zhang and Joseph. R. Lakowicz

Aspirin inhibited the metastasis of colon cancer cells by inhibiting the expression of toll-like receptor 4

Authors: Suaka Kagbo-Kue, Taiwo Ajose and Nicolas Bakinde

RINCK-mediated monoubiquitination of cGAS promotes antiviral innate immune responses

As an important danger signal, the presence of DNA in cytoplasm triggers potent immune responses. Cyclic GMP-AMP synthase (cGAS) is a recently characterized key sensor for cytoplasmic DNA. The engagement of cG...

Authors: Zhao-Shan Liu, Zi-Yu Zhang, Hong Cai, Ming Zhao, Jie Mao, Jiang Dai, Tian Xia, Xue-Min Zhang and Tao Li

Tumor cells versus host immune cells: whose PD-L1 contributes to PD-1/PD-L1 blockade mediated cancer immunotherapy?

Antibody blockade of the PD-1/PD-L1 pathway has elicited durable antitumor responses in the therapy of a broad spectrum of cancers. PD-L1 is constitutively expressed in certain tumors and host immune cells, an...

Authors: Fei Tang and Pan Zheng