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The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation
Cell & Bioscience volume 2, Article number: 30 (2012)
Hypoxia is associated with many disease conditions in humans, such as cancer, stroke and traumatic injuries. Hypoxia elicits broad molecular and cellular changes in diverse eukaryotes. Our recent studies suggest that one likely mechanism mediating such broad changes is through changes in the cellular localization of important regulatory proteins. Particularly, we have found that over 120 nuclear proteins with important functions ranging from transcriptional regulation to RNA processing exhibit altered cellular locations under hypoxia. In this report, we describe further experiments to identify and evaluate the role of nuclear protein relocalization in mediating hypoxia responses in yeast.
To identify regulatory proteins that play a causal role in mediating hypoxia responses, we characterized the time courses of relocalization of hypoxia-altered nuclear proteins in response to hypoxia and reoxygenation. We found that 17 nuclear proteins relocalized in a significantly shorter time period in response to both hypoxia and reoxygenation. Particularly, several components of the SWI/SNF complex were fast responders, and analysis of gene expression data show that many targets of the SWI/SNF proteins are oxygen regulated. Furthermore, confocal fluorescent live cell imaging showed that over 95% of hypoxia-altered SWI/SNF proteins accumulated in the cytosol in hypoxic cells, while over 95% of the proteins were nuclear in normoxic cells, as expected.
SWI/SNF proteins relocalize in response to hypoxia and reoxygenation in a quick manner, and their relocalization likely accounts for, in part or in whole, oxygen regulation of many SWI/SNF target genes.
Living organisms ranging from yeast to mammals use oxygen to generate their cellular energy supply and to synthesize important biomolecules. Hence, they need to respond effectively to changes in oxygen levels in the environment, particularly to hypoxia[1, 2]. In humans, hypoxia is responsible for death or damage by the ischemia accompanying heart attack, stroke, and traumatic injuries[3–5]. The molecular and cellular events induced by changes in oxygen levels are very broad in eukaryotes. For example, over 20% of yeast genes change their transcript levels in response to hypoxia. In the human arterial endothelial cells, more than 8% of all genes alter their transcript levels by at least 1.5-fold in response to hypoxia. In the human primary astrocytes, more than 5% of the genes alter their transcript levels by at least 2-fold in response to hypoxia. Such broad changes in gene expression likely involve coordinated actions of multiple pathways and regulators.
Previous studies have identified several transcriptional regulators, including Mga1 and Rox1, that can mediate oxygen regulation of gene expression in the yeast Saccharomyces cerevisiae[9, 10]. However, these regulators can account for the regulation of only a fraction of hypoxia-regulated genes. Many other regulators are likely involved in mediating oxygen regulation. Recently, in an effort to systematically identify proteins that can mediate oxygen regulation and signaling, we performed a genome-wide screen for proteins that exhibit altered cellular distribution patterns in response to hypoxia and reoxygenation. We found that over 200 proteins alter their cellular locations in response to hypoxia. Particularly, under hypoxia, a good number (at least 121) of nuclear proteins do not localize to the nucleus, but accumulate in the cytosol. In response to reoxygenation, they readily localize to the nucleus. Notably, many of these hypoxia-redistributed nuclear proteins are subunits of key regulatory complexes involved in chromatin remodeling (such as the SWI/SNF complex)[12–14], in transcriptional regulation (such as the SAGA complex), and in splicing (such as the MRP complex). Hence, it is conceivable that some of these complexes can play a dominant role in mediating oxygen regulation of gene expression.
To further assess the roles of these regulators in mediating oxygen signaling and regulation, we examined the time course characteristics of the relocalization of these proteins in response to hypoxia and reoxygenation. We found a small group of nuclear proteins relocalized in a significantly shorter time period in response to both hypoxia and reoxygenation, when compared to other proteins. These proteins include three components of the SWI/SNF complex. Furthermore, using confocal fluorescent imaging of live cells, we quantitatively characterized the effect of hypoxia on the distribution of SWI/SNF proteins. We found that in live hypoxic cells, over 95% of Swi3, Snf5, Snf6, Snf11, Snf12 and Swp82 were in the cytosol, while over 95% of hypoxia-unaffected proteins, such as Swi2 and Taf14, were in the nucleus. These results suggest that hypoxia can significantly alter the composition and property of the SWI/SNF complex and mediate oxygen regulation of gene expression.
Among the hypoxia-redistributed nuclear proteins we previously identified, some are likely involved in mediating oxygen signaling and regulation of gene expression. Particularly, proteins that change their locations in relatively shorter time periods are likely the regulators that initiate further downstream events in responses to hypoxia and reoxygenation. In other words, they are likely to be positioned in the upstream of the hierarchy of the molecular events elicited by hypoxia or reoxygenation, and are responsible for initiating downstream changes such as those in gene expression. We therefore decided to characterize the time course response of the hypoxia-redistributed nuclear proteins in response to hypoxia and reoxygenation.
First, we examined the time course characteristics of nuclear proteins in response to hypoxia. We found that all proteins became predominantly cytosolic after exposure to hypoxia for 12 hours; see Snf11 in Figure1A for an example. One group of these proteins became predominantly cytosolic after only 6 hours or shorter times; see Swp82 in Figure1A for an example. This group has 48 proteins (see Table1). They include several transcriptional regulators and regulators of chromatin, DNA replication and repair, and RNA processing (Figure2). Notably, five components of the SWI/SNF complex relocalized in 6 hours (see Table1 and Figure2), suggesting that they may have a signaling role in initiating downstream events.
Second, we characterized the changes in protein distribution when cells grown under hypoxia were exposed to oxygen. We found that 76 hypoxia-redistributed nuclear proteins (see Table2) recovered their nuclear locations in the majority of cells in one hour; see Swi3 in Figure1B for an example. The rest of the proteins recovered their nuclear location in the majority of the cells in 2 or more hours; see Snf5 in Figure1B for an example. Among these nuclear proteins, 17 of them responded to both hypoxia and reoxygenation in shorter times than the rest of the proteins (see Figure3). Notably, 3 of these faster responding proteins are components of the SWI/SNF complex (Figure3). These and previous results strongly suggest that the SWI/SNF proteins play regulatory roles in mediating oxygen regulation and hypoxia response. Given their roles in chromatin remodeling and transcriptional regulation[17, 18], they are likely responsible for initiating certain changes in gene expression in response to changes in oxygen levels. Although 14 other proteins also responded to hypoxia and reoxygenation in shorter times, they are generally not components of one regulatory complex (Figure3).
Therefore, we decided to further characterize the effect of hypoxia on the SWI/SNF proteins. First, we examined if changes in oxygen levels affect the protein levels of SWI/SNF proteins. To this end, we detected and compared the levels of SWI/SNF proteins in hypoxic and normoxic cells. We used yeast strains expressing the SWI/SNF proteins with the TAP tag at the C-terminus from the natural chromosomal locations. We found that the levels of all detected SWI/SNF proteins were not significantly affected by hypoxia (Figure4). The variations in the ratios of protein levels in hypoxic vs. normoxic cells were generally less than 30%, suggesting that hypoxia did not cause significant degradation of the Swi/Snf proteins during the time period when the proteins would be relocalized to the cytosol. These proteins include those whose cellular location was affected by hypoxia, such as Snf6, Swi3, Swp82 and Snf11 (see Figure4). They also include all those SWI/SNF proteins whose localization was not affected by hypoxia. These results show that the levels of SWI/SNF proteins are not regulated by oxygen levels.
Therefore, the regulation of nuclear localization is likely the dominant mechanism mediating oxygen regulation of SWI/SNF proteins and the regulation of their targets. To further confirm the regulation of nuclear localization of the SWI/SNF proteins by oxygen, we quantitatively examined and compared their distribution in live hypoxic and normoxic cells, by using confocal fluorescent live cell imaging. As expected, for the SWI/SNF proteins whose localization was not affected by oxygen levels, over 95% of the proteins was present in the nucleus in both normoxic and hypoxic cells (see Figure5). Figure5A shows the distribution of Taf14 in air and under hypoxia, while Figure5B shows the distribution of Swi2. For those proteins whose localization was affected by oxygen, over 95% of the proteins was present in the nucleus in air, whereas over 95% of the proteins was present in the cytosol under hypoxia (Figures6 and7). Figure6A shows the distribution of Swi3 in normoxic cells, while Figure6B shows the distribution of Swi3 in hypoxic cells. We also quantified the distribution of other hypoxia-relocalized SWI/SNF proteins (Figure7). Figure7A-E show the distribution of Snf5, Snf6, Snf11, Snf12 and Swp82 in hypoxic cells (The images for normoxic cells invariably showed nuclear localization, as expected and as shown in Figures5 and6, and are therefore omitted). Clearly, Swi2, Snf5, Snf6, Snf11, Snf12 and Swp82 proteins were transported to the nucleus in normoxic cells, but they accumulated in the cytosol in hypoxic cells.
To further ascertain the role of SWI/SNF proteins in oxygen regulation of gene expression, we determined if and how many oxygen-regulated genes are SWI/SNF protein targets as well. To this end, we used our previous microarray and computational work analyzing genes regulated by oxygen/ and Δ hap1 cells. We also used the previously identified targets of 263 transcription factors. Using these two sets of data and the R program, we identified those hypoxia-regulated genes that are targets of SWI/SNF proteins and calculated the p-values. Table3 shows that in the wild type HAP1 cells, 95, 112, 67, 109, 9 and 19 target genes of Swi2, Swi3, Snf5, Snf6, Snf11 and Taf14, respectively, are oxygen regulated. In Δ hap1 cells, similar numbers of these SWI/SNF targets are hypoxia altered. These results strongly suggest that SWI/SNF proteins play a major role in mediating oxygen regulation and hypoxia responses. Furthermore, the changes in the relocalization of SWI/SNF proteins in response to hypoxia are completed between 6–12 hours or 1–2 generations; and the changes in the relocalization of SWI/SNF proteins in response to reoxygenation are completed in less than one generation. In contrast, the transcriptome response to hypoxia are completed after 5–6 generations; and the transcriptome response to reoxygenation are completed in 2 generations. These results show that changes in SWI/SNF protein localization precede transcriptome responses. They therefore strongly suggest that oxygen regulation of SWI/SNF protein localization contribute to, at least in part, oxygen regulation of gene expression.
The SWI/SNF complex is an ATP-dependent chromatin remodeling complex. Its composition and function are conserved from yeast to humans. In yeast, more than 10% of the genes are the targets of SWI/SNF proteins, although the targets of different SWI/SNF proteins are different. Hypoxia and reoxygenation induce changes in gene expression in over 20% of yeast genes. Such broad changes in gene expression involve the action of an array of regulators. Previous studies have shown that Mga2, Rox1, Hap1 and Mot3 are all involved in mediating oxygen regulation of several subsets of genes[6, 21, 23, 24]. In this report, we show that several SWI/SNF proteins alter their subcellular localization readily in response to hypoxia or reoxygenation and that this change in subcellular localization likely contributes to oxygen regulation of SWI/SNF target genes.
Because the targets of SWI/SNF proteins overlap but are not identical, it is likely that different SWI/SNF proteins act on different groups of genes and control their expression. Here, we show that six SWI/SNF proteins accumulate in the cytosol in hypoxic cells, and relocalize to the nucleus in response to reoxygenation. Furthermore, several of the SWI/SNF proteins respond to hypoxia or reoxygenation and relocalize in a relatively quick manner. The redistribution of the six SWI/SNF proteins in the cytosol should presumably change the composition, and thereby the function or selectivity of the SWI/SNF complexes in the nucleus. Hence, the relocalization can affect the target expression of not only these SWI/SNF proteins whose localization is altered by hypoxia, such as Swi3, but also those whose localization is not affected by hypoxia, such as Swi2 and Taf14 (Table3). Very likely, under hypoxia, because Swi3 and other proteins are predominantly present in the cytosol, the nuclear SWI/SNF proteins, such as Swi2, are likely complexed with other proteins, and act as chromatin remodelers on different sets of target genes. This explains why many SWI/SNF target genes are altered by hypoxia/reoxygenation (Table3). Previous studies showed that Swi2, Arp7 and Arp9 form a core subcomplex possessing the ATP-dependent remodeling activity, while Swi3 controls SWI/SNF assembly, ATP-dependent H2A-H2B displacement, as well as recruitment to target genes[25, 26]. Notably, the nuclear localization of Swi2, Arp7 and Arp9 is not affected by hypoxia, while Swi3 is affected. This supports the idea that the core complex can associate with other as yet unidentified proteins and form a different kind of SWI/SNF complexes in the nucleus in hypoxic cells.
These results suggest a model for how oxygen may modulate SWI/SNF complex composition and function (Figure8). In normoxic cells, SWI/SNF components form complexes in the nucleus and remodel chromatin structure at their target genes. In hypoxic cells Swi3 and other five SWI/SNF proteins accumulate in the cytosol, leaving the Swi2-Arp7-Arp9 core subcomplex available to interact with other proteins. Consequently, a different kind of SWI/SNF complex containing the core complex and other proteins (A, B and C in Figure8) can be formed and act to remodel chromatin and control gene expression in different sets of genes. In response to reoxygenation, Swi3 and other proteins can readily relocalize to the nucleus, forming the normoxic SWI/SNF complexes, and re-establish gene expression patterns under normoxic conditions.
This model is also consistent with a recent study showing that Swi3 is a key regulator in controlling respiration genes. The authors used a computational approach to analyze modules of genes with a common regulation that are affected by specific DNA polymorphisms. They integrated genotypic and expression data for individuals in a segregating population with complementary expression data of strains mutated in a variety of regulatory proteins, in order to identify regulatory-linkage modules. In so doing, they found that Swi3 is a dominant regulator in the control of respiratory gene expression. The effect of swi3 deletion is stronger than that of known respiratory regulators, including Hap2/3/4/5, Mot3 and Rox1. This is in complete agreement with our results showing that hundreds of SWI/SNF targets are altered by hypoxia (Table3), and supports our model (Figure8). The regulation of SWI/SNF protein localization may also occur in other eukaryotes. For example, in mammalian cells, recent studies showed that SWI/SNF proteins are important for oxygen regulation in mammalian cells[28, 29]. It is likely that SWI/SNF proteins can respond to changes in oxygen levels and regulate gene expression in diverse eukaryotes.
Several SWI/SNF proteins, including Swi3, Snf6 and Swp82, respond to hypoxia or reoxygenation and alter their subcellular distribution in a relatively quick manner. This change in localization likely contributes to oxygen regulation of SWI/SNF target genes.
Yeast strains and antibodies
The yeast GFP clone collection of 4159 strains expressing GFP-tagged proteins was purchased from Invitrogen Corp. The anti-TAP monoclonal antibody was purchased from Open Biosystems.
The creation of hypoxic growth conditions
Hypoxic (~10 ppb O2) growth condition was created by using a hypoxia chamber (Coy Laboratory, Inc.) and by filling the chamber with a mixture of 5% H2 and 95% N2 in the presence of a palladium catalyst. The oxygen level in the chamber was monitored by using the Model 10 gas analyzer (Coy Laboratory, Inc.). The precise level of oxygen was also estimated by using a CHEMetrics rhodazine oxygen detection kit (K-7511) with the minimum detection limit at 1 ppb, and a range of 0–20 ppb. The hypoxic state was further confirmed by measuring oxygen-controlled promoter activities, including UAS1/CYC1, ANB1 and OLE1[9, 10, 31].
Time course characterization of cellular localization of SWI/SNF proteins
For a time course characterization of SWI/SNF protein relocalization in response to hypoxia or reoxygenation, we used a previously defined nuclear protein import assay in yeast[32–34]. Briefly, cells expressing GFP-tagged proteins at various time points of hypoxia or reoxygenation treatment were collected, and images were acquired. At least 25 cells were counted at each time point, and three sets of cells were counted. A particular cell was counted as having the GFP-tagged protein in the nucleus if the nucleus was much brighter than the surrounding cytoplasm and a clear nuclear-cytoplasmic boundary was visible. Cells with excessive bright or weak fluorescence or with aberrant morphology were not scored.
Confocal fluorescent live cell imaging and quantitation
GFP-tagged strains were grown in synthetic complete media in air or in a hypoxia chamber. Cells were collected and subjected to confocal fluorescent imaging and quantitation. Image acquisition of live cells was performed by using a Perkin Elmer UltraView ERS Spinning Disc Confocal Microscope (Perkin Elmer, Waltham, MA) with a Zeiss 100x/1.4 Oil Immersion objective (Carl Zeiss, Thornwood, NY). High speed images were captured by using a Hamamatsu EMCCD C9100 digital camera (Hamamatsu Corporation, Bridgewater, NJ). Z-stacks were recorded for the DAPI channel (EX 405nm) and the GFP channel (EX 488nm) by moving the objective turret with a UltraView z-focus drive (Perkin Elmer, Waltham, MA). Volocity 5.4.2 (Improvision, Perkin Elmer, MA, USA) was used for image acquisition. The 3D confocal images were analyzed, and statistical data were collected by using Imaris 7.4.0 (Bitplane, South Windsor, CT).
Preparation of yeast cell extracts and Western blotting
Yeast cells expressing various TAP-tagged proteins were grown to an optical density (OD600) of approximately 0.8. Cells were harvested and resuspended in 3 packed cell volumes of buffer (20 mM Tris, 10 mM MgCl2, 1 mM EDTA, 10% glycerol, 1 mM dithiothreitol, 0.3 M NaCl, 1 mM phenylmethylsulfonyl fluoride, 1 mg of pepstatin per ml, 1 mg of leupeptin per ml). Cells were then permeabilized by agitation with 4 packed cell volumes of glass beads, and extracts were collected as described previously. Protein concentrations were determined by the BCA (bicinchoninic acid) protein assay kit (Pierce).
For Western blotting, approximately 100 μg of whole-cell extracts were first separated on 8% sodium dodecyl sulfate (SDS)–polyacrylamide gels and then transferred to polyvinylidene difluoride or nitrocellulose membranes (Bio-Rad Laboratories). TAP-tagged proteins were detected by using a monoclonal antibody against TAP and a chemiluminescence Western blotting kit (Roche Diagnostics). The signals were detected and quantified by using a Kodak image station 4000MM Pro with the molecular imaging software, version 4.5.
Protein GO analysis and construction of the network map
The analysis of functional categories of relocalized proteins was performed on Funspec (http://funspec.med.utoronto.ca/). For constructing the protein network map, the Cytoscape application program (http://www.cytoscape.org/) was used. The faster responding proteins identified were mapped according to their GO terms. They were obtained by using the SGD Gene Ontology Slim Mapper Web Tool set to "Macromolecular Complex terms: Components" on the SGD website. The mapped output file was reformatted into a Cytoscape compatible network file, and the network map was created. The network map was further graphically refined by using the Canvas application program. The subcellular compartments of these proteins in normoxic cells were designated based on data from the O'Shea lab.
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This work was supported by NIH grant GM62246 (LZ). We would like to acknowledge the assistance of the UT Southwestern Live Cell Imaging Facility, a Shared Resource of the Harold C. Simmons Cancer Center, supported in part by an NCI Cancer Center Support Grant, 1P30 CA142543-01.
The authors declare that they have no competing interests.
RGD, TMC and RMK performed time course studies and constructed network maps. JH and AS performed confocal fluorescent imaging and quantitation and analysis of regulator targets. LZ conceived of the study and drafted the manuscript. All authors read and approved the final manuscript.
Ranita Ghosh Dastidar, Jagmohan Hooda, Ajit Shah contributed equally to this work.
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Dastidar, R.G., Hooda, J., Shah, A. et al. The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation. Cell Biosci 2, 30 (2012). https://doi.org/10.1186/2045-3701-2-30
- Hypoxia response
- Oxygen regulation
- Live cell imaging
- Protein localization