Figure 4

Western blot showing TAP-tagged proteins in extracts prepared from normoxic and hypoxic cells. Shown here are proteins in extracts from the parent BY4741 cells without any TAP-tagged proteins expressed (N), and from cells grown in air (A) or under hypoxia (H) which expresses Snf6-TAP (molecular mass: 58 kDa), Swi3-TAP (113 kDa), Swp82-TAP (90 kDa), Snf11-TAP (40 kDa), Swi1-TAP (168 kDa), Swi2-TAP (214 kDa), Arp7-TAP (74 kDa), Arp9-TAP (73 kDa), Taf14-TAP (47 kDa), respectively. For the hypoxic condition, cells were placed in a hypoxia chamber for up to 12 hours (the time period necessary for the proteins to relocate to the cytosol). The intensity of bands representing the Swi/Snf proteins was quantified, and the intensity ratios of the bands representing the Swi/Snf proteins in hypoxic vs. normoxic cells were plotted and shown below the Western blot images. The data plotted are averages of three replicates.