Skip to main content
Download PDF

Slit diaphragm maintenance requires dynamic clathrin-mediated endocytosis facilitated by AP-2, Lap, Aux and Hsc70-4 in nephrocytes

Cell & Bioscience volume 11, Article number: 83 (2021) Cite this article

Abstract

Background

The Slit diaphragm (SD) is the key filtration structure in human glomerular kidney that is affected in many types of renal diseases. SD proteins are known to undergo endocytosis and recycling to maintain the integrity of the filtration structure. However, the key components of this pathway remain unclear.

Methods

Using the Drosophila nephrocyte as a genetic screen platform, we screened most genes involved in endocytosis and cell trafficking, and identified the key components of the cell trafficking pathway required for SD protein endocytosis and recycling.

Results

We discovered that the SD protein endocytosis and recycling pathway contains clathrin, dynamin, AP-2 complex, like-AP180 (Lap), auxilin and Hsc70-4 (the endocytosis part) followed by Rab11 and the exocyst complex (the recycling part). Disrupting any component in this pathway led to disrupted SD on the cell surface and intracellular accumulation of mislocalized SD proteins. We also showed the first in vivo evidence of trapped SD proteins in clathrin-coated pits at the plasma membrane when this pathway is disrupted.

Conclusions

All genes in this SD protein endocytosis and recycling pathway, as well as SD proteins themselves, are highly conserved from flies to humans. Thus, our results suggest that the SD proteins in human kidney undergo the same endocytosis and recycling pathway to maintain the filtration structure, and mutations in any genes in this pathway could lead to abnormal SD and renal diseases.

Introduction

The glomerular filtration barrier, which contains the fenestrated endothelium of the glomerular capillaries, the glomerular basement membranes and podocytes, prevents passage of the majority of proteins, such as albumin, from blood into urine. Damages to the glomerulus causes proteinuria, wherein proteins in the blood leak into the urine, and may progress into kidney failure. The mammalian podocyte slit diaphragm structure is a major component of the glomerular filtration barrier, indeed its disruption has been associated with proteinuria and many types of renal diseases [21]

Nephrin, a transmembrane protein encoded by the NPHS1 gene [15], plays an essential role in assembly of the slit diaphragm structure and also functions as a signaling platform regulating the podocyte actin cytoskeleton, membrane trafficking and calcium mechano-signaling [17]. Mutations in NPHS1 disrupt slit diaphragm structure, cause foot process effacement and lead to severe proteinuria and nephrotic syndrome [15, 26]. Perturbations in nephrin protein level and localization have been found in podocytes of patients with membranous glomerulonephritis (GN), minimal change GN and diabetic nephropathy [4, 5], suggesting regulation of nephrin is critical for slit diaphragm function. The cytoplasmic tail of nephrin, when phosphorylated by Src-family tyrosine kinases, can selectively bind Nck adaptor proteins which regulate the cytoskeleton, thus linking nephrin to the podocyte actin cytoskeleton [13, 30].

In cultured cells, nephrin, when dephosphorylated at the conserved Y1193 residue, was shown to interact with β-Arrestin2 and to undergo endocytosis to attenuate its signaling [23]. Similarly in COS-7 cells, nephrin was found to be endocytosed through both clathrin-mediated endocytosis (CME) and raft-mediated endocytosis (RME), which is clathrin-independent yet like CME requires dynamin activity [22]. Using different pharmacological inhibitors, Waters et al. found that Notch promotes dynamin-dependent, raft-independent endocytosis of nephrin [33]. The above studies were carried out in cultured cells, therefore while the results have provided possible mechanisms in the regulation of nephrin endocytosis, it remained unclear whether nephrin and other slit diaphragm proteins are regulated similarly in podocytes in vivo.

It has been shown in mice, that podocyte-specific ablation of dynamin, synaptojanin and endophilin—all three critical components of clathrin-mediated endocytosis—results in endocytic defects, foot process effacement, severe proteinuria and kidney failure [25]. Recently, disease-associated variants in other endocytosis/recycling pathway genes, like GAPVD1, ANKFY1, TBC1D8B and EXOC4, have been identified in patients with nephrotic syndrome, suggesting that endocytosis and recycling play an important role in glomerular function [3, 12, 14]. However, it is challenging to systematically examine the localization of nephrin proteins in mammalian kidney samples with endocytic/recycling mutations at high resolution [27, 28], thereby limiting the scope of the mammalian system. As such it remains unclear whether the endocytosis/recycling pathway directly regulates the dynamics of slit diaphragm proteins in vivo.

The Drosophila nephrocyte, fly equivalent of podocyte, provides an excellent model system to study the dynamics of slit diaphragm proteins in vivo. We and others have found that over 85% of the genes involved in human steroid resistant nephrotic syndrome have functional homologs in Drosophila nephrocytes [6, 11]. The Drosophila nephrocyte slit diaphragm (NSD) has been shown to share functional, molecular and ultrastructural similarities to the mammalian podocyte slit diaphragm [34, 37]. The distribution of Drosophila NSD proteins at nephrocyte plasma membrane is highly organized in a fingerprint-like pattern and is sensitive to genetic perturbations as in mammals [11, 34], providing an excellent assay to probe the conserved mechanisms to regulate the dynamics of slit diaphragm proteins. Moreover, we recently established an endocytosis and recycling model for slit diaphragm proteins in Drosophila [35]. We showed that silencing the critical players in the endocytosis and recycling pathways led to severe defects in nephrocyte function, disruption of slit diaphragm protein localization and loss of the NSD ultrastructure. For example, the exocyst proteins, which form a conserved complex tethering exocytosis vesicles or recycling endosomes to the plasma membrane, are required for slit diaphragm protein localization in nephrocytes [35], which is consistent with the results that mutations of human EXOC4 and mouse EXOC5 lead to severely impaired kidney filtration [19]. Silencing Rab5 and Rab11, which are required for the early and late endosome functions, respectively, led to slit diaphragm protein mislocalization. We proposed a working model in which slit diaphragm proteins are endocytosed from NSD structures at the nephrocyte plasma membrane, then enter the Rab5-dependent early endosome and are subsequently sorted into Rab11-dependent recycling endosomes. Aided by the exocyst complex, the recycling endosome was tethered to the surface membrane, and via membrane fusion returned the slit diaphragm proteins once again to the NSD structures.

While CME is the dominant and well-studied endocytic route, CIE routes, which include caveolar pathway, clathrin-independent carriers/GPI-AP enriched early endosomal compartments (CLIC/GEEC) pathway and other pathways, are emerging as important regulators of cell growth and development. Since vesicles from both CME and CIE pathways can enter the early endosomes, it was not clear which pathway, CME or CIE, is required for slit diaphragm protein endocytosis and NSD structure integrity. Here, we screened candidate genes involved in both CME and CIE in the nephrocytes and found that the CME pathway is essential for maintenance of the NSD structure.

Results

Clathrin is required for the endocytosis of slit diaphragm proteins in nephrocytes

Our previous work showed that silencing genes required for slit diaphragm proteins endocytosis and recycling disrupts NSD structures and leads to mislocalization of slit diaphragm proteins (like Sns and Pyd, the fly homologs of Nephrin and ZO-1, respectively). Utilizing this specific phenotype, we systematically screened candidate endocytic genes for their roles in the NSD structure maintenance (Additional file 1: Table S1). We used the Dot-Gal4 nephrocyte-specific driver to express the RNA knock down transgenes, UAS-RNAi. The knockdown was usually efficient and specific, as shown by antibody staining of several proteins (Additional file 1: Figure S4 and Figure S10). The clathrin-independent endocytosis (CIE) is relatively understudied in Drosophila. For example, there is no fly homolog for Caveolin, so it is unclear whether fly has caveolar endocytosis pathway. And many CIE routes identified in mammalian cells have not been studied in Drosophila cells [9]. However, the CLIC/GEEC pathway, which is the route for uptake of glycosylphosphatidylinositol-anchored proteins (GPI-APs) and fluid phase, is conserved and the only CIE route that has been molecularly characterized and confirmed in Drosophila [7, 9, 10]. We tested the phenotype of RNAi knockdown of the critical factor in the CLIC/GEEC pathway, Arf79F, fly homolog of mammalian ARF1, in nephrocytes [7, 16]. In wild type nephrocytes imaged in medial plane section across the nucleus, immune-labeling of Pyd revealed its strong association with cell membrane, with few signal inside the cell (Fig. 1a; on the cell surface section, Pyd showed a uniform and smoothly distributed fingerprint-like localization pattern (Fig. 1a′–a′′). Silencing Arf79F did not affect the lines of the fingerprint pattern of Pyd localization, but the gap between the lines increased significantly, causing reduced total surface density of Pyd (Fig. 1b′–b′′). However, Pyd protein did not accumulate inside the nephrocytes (Fig. 1b). This phenotype is different from known genes involved in NSD endocytosis and recycling, such as exocyst [35]. As Arf79F is also involved in other cellular processes such as Golgi trafficking, it is unclear whether this Pyd phenotype was caused by defects in the CLIC/GEEC pathway.

Fig. 1
figure 1

The clathrin complex is required for slit diaphragm protein endocytosis. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. a–a″ Wild type (Control) adult fly nephrocytes exhibited Pyd (red) localization by immunofluorescence labeling that was tightly localized to the cell margin, defining a highly regular, continuous circumferential ring when examined by confocal microscopy (a, medial optical section). Visualization of Pyd at the cell surface plane showed a highly regular and uniform pattern of distribution (a′–a″). Cell nuclei were labeled by DAPI (blue). Scale bar: 1 μm. b Silencing of Arf79f (b–b″) did not cause intracellular accumulation of Pyd proteins. But on the cell surface section, the distance between the lines of Pyd fingerprint become wider. c–d Silencing of Chc (c–c″) or Clc (d–d″) genes of the clathrin complex resulted in a severe disruption of normal Pyd localization at the nephrocyte cell margin and the cell surface. Intracellular Pyd showed a distribution with aggregate-like concentrates. The fingerprint pattern of Pyd at the cell surface was also disrupted: the highly organized and evenly spaced lines were gone, replaced by irregular short lines or aggregates

Full size image

To directly test the role of CME in slit diaphragm protein endocytosis, we silenced Clathrin heavy chain (Chc) and Clathrin light chain (Clc) in nephrocytes. The lack of Chc or Clc resulted in mislocalized slit diaphragm protein Pyd. Inside the nephrocytes, aggregates were accumulated; on the cell surface, the characteristic Pyd fingerprint-like pattern was disrupted and some aggregates were formed (Fig. 1c–d′′). The localization of another slit diaphragm protein Sns was similarly disrupted (Additional file 1: Figure S2).The phenotype of clathrin deficient nephrocytes is highly similar to that of nephrocytes deficient with exocyst genes [35], suggesting they function in the same pathway for slit diaphragm protein endocytosis and recycling. The above results demonstrate that clathrin is directly required for slit diaphragm protein endocytosis.

Fig. 2
figure 2

The endocytosis regulator, Shi, is required for nephrocyte function and localization of slit diaphragm protein. a Immunofluorescent labeling in wild type (Control) adult fly nephrocytes exhibited tight co-localization of Pyd (green) and Sns (red) at the cell margin, defining a fine and sharply continuous ring when examined by confocal microscopy (a, medial optical section). Detection of Pyd and Sns (a′–a″) at the cell surface plane showed a highly regular and fingerprint-like distribution pattern. Cell nuclei are labeled by DAPI (blue). Scale bar: 1 μm. b–b″ Immunofluorescent labeling in shi-silenced (shi-IR) adult fly nephrocytes revealed disturbed Pyd and Sns localization at the nephrocyte cell margin and were found to have accumulated intracellularly with appearance of aggregates (b). The characteristic cell surface localization had been severely disrupted, visible as dots of seemingly Pyd and Sns aggregates and overall cell surface expression of Pyd and Sns was greatly reduced (b′–b″). c–d Immunofluorescence labeling of Pyd (green) and Clc (red). In wild type (Control) nephrocytes (c–c″), Pyd localized at the cell membrane. Clc localized to vesicles adjacent to the cell membrane. In shi-silenced (shi-IR) nephrocytes (d–d″), Pyd was mostly absent at the cell membrane, but can be occationally found in extraordinary large Clathrin-coated vesicles (arrowhead). Scale bar: 1 μm

Full size image

We tested whether clathrin is required for nephrocyte endocytosis and reabsorption function by in vivo protein uptake assay. In flies carrying MHC-ANF-RFP, the myosin heavy chain (MHC) promoter directs muscle cell expression of a rat atrium natriuretic factor (ANF)–red fluorescent protein (RFP) fusion protein (ANF-RFP) that is secreted into the hemolymph. ANF-RFP is typically taken up by healthy, wild type nephrocytes and the intracellular red fluorescence can be readily visualized and quantitated in vivo (Additional file 1: Figure S1A). Silencing of chc or clc led to severely reduced levels of RFP fluorescence within nephrocytes (Additional file 1: Figure S1A, C), indicating that clathrin is required for the hemolymph protein filtering/absorption functions of nephrocyte. In an independent ex vivo functional assay, we tested the ability of dissected nephrocytes to absorb Texas Red-labeled 10 kD Dextran particles. Again, silencing of chc or clc induced a marked deficiency in intracellular Texas Red fluorescence compared to control wildtype nephrocytes (Additional file 1: Figure S1B, D). These observations indicate that clathrin is required for normal nephrocyte function.

Drosophila shibire, the fly homolog of dynamin, is required for slit diaphragm protein endocytosis

Dynamin, a critical component of CME and some routes of CIE, has been shown to be essential for podocyte function in mice [25]. Its fly homolog Shibire (Shi) is highly conserved and plays important roles in vesicular transport, such as endocytosis, in Drosophila [1, 29, 31, 32]. In our screen, we found Shi is also required for slit diaphragm protein endocytosis. Silencing shi led to disruption of Sns/Pyd localization: Sns/Pyd aggregated in puncta both inside nephrocytes (Fig. 2b) and on the cell surface (Fig. 2b′, b″), with less cell surface area was covered with Sns/Pyd, similar phenotypes with silencing other genes required for slit diaphragm protein endocytosis and recycling. The results indicate that Shi is required for the maintenance of slit diaphragm structure, through the endocytosis of slit diaphragm proteins.

We tested whether Shi is required for nephrocyte function by nephrocyte-specific silencing of shi, followed by in vivo protein uptake assay to examine effects on nephrocyte functioning. Silencing of shi led to severely reduced levels of RFP fluorescence within nephrocytes (Additional file 1: Figure S3A, B), indicating that similar to dynamin, Shi is required for the hemolymph protein filtering/absorption functions of nephrocyte. In an independent ex vivo functional assay, we tested the ability of dissected nephrocytes to absorb 10 kD Dextran particles. Again, silencing of shi induced a marked deficiency in intracellular Texas Red fluorescence compared to control wildtype nephrocytes (Additional file 1: Figure S3C, D). These observations indicate that Shi is required for normal nephrocyte function.

We tried to find direct evidence of slit diaphragm protein undergoing clathrin-mediated endocytosis by examining co-localization between the slit diaphragm protein Pyd and Clathrin (Clc). In the wild type nephrocyte, Pyd localized at the cell membrane while Clc appeared localized in vesicles near the cell membrane, with little if any overlapping (Fig. 2c–c″). Endocytosis is a rapid process with only a small portion of slit diaphragm proteins undergoing endocytosis at a given time, making it technically difficult to capture extensive co-localization of Pyd and Clc by imaging. However, we reasoned that by blocking the release of clathrin-coated pits we could catch a snapshot of slit diaphragm proteins undergoing endocytosis. To test this hypothesis, we silenced shi, which is required for fission of clathrin-coated pits, and then examined the localization of Pyd and Clc. Indeed, we were able to capture clear images of the Pyd protein on a small ring circled by a bigger ring with the Clathrin protein on the cytoplasmic side of the nephrocyte membrane (Fig. 2d–d″), providing the first in vivo evidence that slit diaphragm proteins undergo clathrin-mediated endocytosis from the nephrocyte membrane.

AP-2 complex and Lap, clathrin-coated pit assembly proteins, play a role in endocytosis of slit diaphragm proteins

The cargo proteins undergoing CME are known to be recognized either directly by Adaptor Protein complex 2 (AP-2) or by other adaptor proteins. The AP-2 complex contains four subunits, known as AP-2α, AP-2μ, AP-2σ and AP-1-2β in Drosophila. AP-1-2β is shared between the AP-2 and AP-1 complexes. In fly, the AP-2 complex is involved in plasma membrane endocytosis, while the AP-1 complex is specific for trafficking between the trans-Golgi network and endosomes. Furthermore, the AP-2 complex has been shown to bind plasma membrane phospholipid headgroups and the clathrin complex, and to play a critical role in the assembly of the clathrin-coated pits [20].

Depletion of AP-2 genes resulted in severely reduced filtering/absorption capacity in the nephrocytes (Additional file 1: Figure S5), as well as disrupted Sns/Pyd localization visible as aggregate formation both at the surface and intracellular (Fig. 3; Additional file 1: Figure S6), a phenotype highly similar to that observed in the Clathrin (Chc or Clc)-silenced nephrocytes. Since AP-1-2β is present in both AP-1 and AP-2 complexes, we could not rule out that both complexes are required for slit diaphragm structure maintenance. To exclude the involvement of AP-1, we also silenced genes specific to the AP-1 complex (AP-1γ, AP-1σ and AP-1μ) and found that the localization of slit diaphragm proteins was unchanged from control wild type nephrocytes (Additional file 1: Figure S7). These findings indicate the AP-2 complex, not AP-1, is required for slit diaphragm endocytosis.

Fig. 3
figure 3

The AP-2 complex, a regulator of clathrin, is required for endocytosis of slit diaphragm protein Pyd. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. a–a″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′–a″). b–e Silencing of AP-1-2β (b–b″), AP-2μ (c–c″), AP-2α (d–d″) and AP-2σ (e–e″) resulted in severe disruption of Pyd cell surface localization. Revealing intracellular localization of aggregate-like structures, and cell-surface distribution marked by high-density aggregation areas

Full size image

In addition to the AP-2 complex, other adaptor proteins are required for assembly of the clathrin coat. We examine the role of one such protein, AP180, in slit diaphragm endocytosis. Silencing like-AP180 (lap), the Drosophila AP180 homolog, in nephrocytes results in a significant reduction in function (Additional file 1: Figure S8). Interestingly, depleted Lap led to accumulation of slit diaphragm protein (Pyd) in the cytoplasm similar to that seen in AP-2 complex gene silencing (Fig. 4b). However, the pattern of Sns/Pyd on the nephrocyte cell surface changed only slightly when lap was silenced, with the characteristic fingerprint-like pattern largely intact, although we did observe increased gaps between two slits occasionally (Fig. 4b′–b″, Additional file 1: Figure S9). The results suggest Lap is likely required for the optimal assembly of clathrin coat, but it is not critical for CME pathway activity.

Fig. 4
figure 4

Clathrin accessory proteins for assembly (Lap) and disassembly (Aux; Hsc70-4) are required for endocytosis of slit diaphragm protein Pyd. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. a–a″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′–a″). b–b″ Silencing of lap resulted in intracellular aggregation of Pyd proteins with partial disruption of Pyd cell surface localization. Note the fingerprint-like pattern remained largely intact, however gaps between adjacent Pyd-positive ridges appeared increased. c–d Silencing of aux (c–c″) and Hsc70-4 (d–d″) resulted in severe disruption of Pyd cell surface localization. Revealing intracellular localization of aggregate-like structures, and cell-surface distribution marked by high-density aggregation areas

Full size image

The Aux and Hsc70-4 clathrin-coated pit disassembly proteins play a role in endocytosis of slit diaphragm proteins

After fission from the plasma membrane, the clathrin-coated vesicles are recognized by auxilin, which then recruits and activates ATPase Hsc70 to remove the clathrin coat. Disassembly of the clathrin coat frees the AP-2 complex, clathrin and Shi (Dynamin) to participate in the next cycle of endocytosis. To examine the role of auxilin and Hsc70 in slit diaphragm protein endocytosis, we silenced their Drosophila homologs, aux and Hsc70-4, in nephrocytes. We found that silencing Drosophila homologs, aux and Hsc70-4 in the nephrocytes showed highly similar phenotypes to those of Clathrin (Chc or Clc)-silenced nephrocytes, including a significant reduction in their filtering/absorption function (Additional file 1: Figure S8), and disrupted location of slit diaphragm proteins (Pyd and Sns) (Fig. 4c–d″, Additional file 1: Figure S9). The above results suggest that Aux and Hsc70-4 are critical for clathrin-mediated endocytosis of silt diaphragm proteins.

Slit diaphragm structure maintenance requires clathrin-mediated endocytosis

The data above were obtained using a Dot-Gal4 driver to silence the gene of interest. However, as this driver is already expressed early in fly development, the results might reflect the combined effects of early slit diaphragm formation during development and later maintenance after maturation. To test the requirement of CME in slit diaphragm maintenance specifically, we used the temperature sensitive Gal80 to control the activity of Dot-Gal4 (Fig. 5a). In this system, Gal80 activity can be controlled by the environmental temperature at which the flies are kept. Gal80 is driven by the ubiquitous tubulin promoter and when expressed binds and represses the transcriptional activator Gal4. Fly embryos were kept at 18 °C (active Gal80 inhibits Gal4) until they eclosed as adult fly, allowing unimpeded maturation of the slit diaphragm structure. The one-day old flies were then transferred to 29 °C for 3 days (inactive Gal80, thus Gal4 drives expression of RNAi constructs), triggering target gene RNA knockdown (Fig. 5a). Using this system to control the knockdown of Clathrin (Clc), at 18 °C the localization of slit diaphragm protein (Pyd) in the nephrocytes of Clc transgenic flies was indistinguishable from that in the control group (Fig. 5b–c′). However, after shifting the flies to 29 °C for 3 days, the localization of slit diaphragm protein Pyd was severely disrupted (Fig. 5d–e′). The fingerprint-like pattern was replaced by disorganization and aggregation, highly similar to that observed with continuous Clc knockdown without Gal80 (Fig. 1d–d″). These results suggest that after slit diaphragm maturation, CME pathway activity is required for slit diaphragm structure maintenance.

Fig. 5
figure 5

Nephrocyte slit diaphragm structure maintenance requires clathrin. a Schematic representation of the temperature sensitive Gal80 experimental system. In brief, at 18 °C Gal80 is active, thus Gal80 inhibits Gal4 thereby preventing transcription. At 29 °C Gal80 no longer binds Gal4, thus Gal4 drives expression of Clc-IR thereby silencing expression of Clc specifically in the nephrocytes. Dorothy (Dot) promoter, nephrocyte-specific expression; tubulin (tub) promoter, ubiquitous expression pattern. b–e Immunofluorescence labeling for Pyd (red) of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. b–c′ At day one adult flies reared at 18 °C, wild type (Control; b–b′) and Clc RNAi knockdown (Clc-IR; c–c′) nephrocytes exhibited indistinguishable Pyd (red) distribution. In both, Pyd exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface. d–e′ At day four, after a three-day shift to 29 °C, nephrocyte cell surface Pyd distribution in wild type (Control) flies remained unchanged, showing the characteristic fingerprint-like pattern (d–d′). Nephrocytes in which the Clc gene was silenced (Clc-IR), displayed severe disruption of Pyd localization at the cell surface (e–e′)

Full size image

Ultrastructural changes in nephrocytes with CME defects

To further establish the role of CME genes in regulating slit diaphragm structures, we examined the ultrastructural changes in Clc- and shi-silenced nephrocytes by TEM. In control nephrocytes, the slit diaphragm and lacuna channel structures are highly organized (Fig. 6a). In comparison, the number of slit diaphragm and lacuna channel structures in Clc-silenced nephrocytes were found to be greatly reduced (Fig. 6b, d). Although Clc depletion inhibits the CME pathway, both vesicles in the process of endocytosis as well as many small vesicles were visible in the Clc-silenced nephrocytes, thereby indicating the presence of a clathrin-independent endocytosis pathway in the nephrocytes. The CIE process also explains the accumulation of slit diaphragm proteins inside the cell in CME-gene-deficient nephrocytes, as these proteins were endocytosed through CIE pathway. The shi-silenced nephrocytes also showed severely decreased numbers of slit diaphragm and lacuna channel structures (Fig. 6c, d). We also frequently observed clathrin-coated pits which appeared unable to fission from the cell membrane in these nephrocytes, consistent with the role of Shi in the scission of clathrin-coated pits. Compared to Clc-silenced nephrocytes, there were much fewer vesicles in the shi-silenced nephrocytes, suggesting some vesicles in Clc depleted nephrocytes arose from clathrin-independent dynamin-dependent endocytosis. The thickness of basement membrane of shi-silenced nephrocyte increased dramatically, compared to both control wild type and Clc-silenced nephrocyte (Fig. 6e), which might be due to the accumulation of hemolymph proteins trapped in the basement membrane. This result suggests that some hemolymph proteins are cleared from the basement membrane through clathrin-independent but Shi (mammalian Dynamin)-dependent endocytosis. The ultrastructural changes in Clc- and shi-silenced nephrocytes are consistent with the role of CME genes in the maintenance of silt diaphragm structure, and also indicate the presence of a CIE pathway in nephrocytes.

Fig. 6
figure 6

Ultrastructural changes in Clc- and shi-silenced nephrocytes. a–c Transmission electron microscopy (TEM) images of fly nephrocytes. Scale bar: 100 nm. a A wild type (Control) fly nephrocyte shows the typical characteristic ultrastructural features of regularly spaced slit diaphragms (black arrow) spanning the openings of lacuna channel membrane invaginations (asterisk). b Clc gene silencing (Clc-IR) eliminated the lacuna channels and slit diaphragm structures in the nephrocyte. Note the presence of many small vesicles as well as a vesicle undergoing endocytosis (arrowhead). c Slit diaphragm and lacuna channel structures were disrupted in shi-silenced (shi-IR) nephrocytes. The thickness of basement membrane increased significantly (see e). Note the presence of many Ω-shaped clathrin-coated pits at the cell membrane (white arrow). d Quantification of the number of slit diaphragms. In the TEM image, the cell membrane was divided into two 1 μm sections, then the number of slit diaphragms within each section was counted. At least seven sections were counted for each genotype. The results are presented as mean ± SD. Results were analyzed by Student’s t-test. ***P < 0.001. Clc-IR and shi-IR nephrocytes showed near total depletion of slit diaphragms. e Quantification of the thickness of the basement membrane. In the TEM image, the thickness of the basement membrane was measured in 1 μm intervals. At least ten measurements were obtained for each genotype. The results are presented as mean ± SD. Results were analyzed by Student’s t-test. ***P < 0.001. Membrane thickness in Clc-IR nephrocytes was unchanged when compared to wild type (Control). On the other hand, the basement membrane after silencing shi (shi-IR) was significantly thicker than that of wild type (Control) nephrocytes

Full size image

The endocytosed slit diaphragm proteins were recycled back to plasma membrane

Vesicles with cargo proteins endocytosed through CME pathway would fuse with early endosomes, the formation of which is controlled by Rab5 GTPase [18]. For most the cargo proteins in the early endosomes, they were sorted into three destinations: the late endosome through Rab7 GTPase, the Golgi through the Retromer complex and the recycling endosome through Rab11 GTPase [18]. We screened genes involved in these processes (Additional file 1: Table S1) and found that silencing Rab5 or Rab11 lead to mislocalization of Pyd protein similar to CME pathway genes, while silencing Rab7 or the Retromer did not obviously alter the localization of Pyd protein (Fig. 7 and data not shown), suggesting Rab5-dependent early endosome and Rab11-dependent recycling endosome are required for slit diaphragm protein endocytosis and recycling. The recycling of some cargo proteins back to plasma membrane does not require Rab11, but may depend on other Rab GTPases like Rab4, Rab10 or Rab35. We also tested these Rabs and found that they were not required for slit diaphragm protein Pyd recycling (data not shown). Our results indicate the slit diaphragm proteins endocytosed by CME pathway were accepted by the early endosome, and then sorted into recycling endosome, with plasma membrane as the final destination.

Fig. 7
figure 7

Genetic analysis of the destination of endocytosed slit diaphragm proteins. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. a–a″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′–a″). b–b″ and e–e″ Silencing of rab5 and rab11 resulted in intracellular aggregation of Pyd proteins and disruption of Pyd cell surface localization. Rab5 is a critical regulator of early endosome formation, while Rab11 is important for recycling endosome function. c–d Silencing of rab7 (c–c″) or vps29 (d–d″) did not disrupt Pyd cell surface localization. Rab7 is critical for late endosome maturation, and Vps29 is a component of the Retromer complex, which is required for early endosome to Golgi retrograde trafficking

Full size image

Discussion

Maintaining the integrity of the slit diaphragm structure is essential for the glomerular filtration function, as illustrated by many nephrotic mutations affecting slit diaphragm proteins. Nephrin has been proposed to undergo clathrin-mediated endocytosis in cultured cells or in mice podocyte in vivo [22, 23, 33]. However, questions remained regarding the role of the CME pathway in regulating the slit diaphragm structure. The data presented here, clearly demonstrate that the clathrin-mediated endocytosis pathway plays a critical role in maintaining the nephrocyte slit diaphragm structure. As components of the CME pathway are highly conserved between fly and human (Additional file 1: Table S1), our results suggest a similar role for CME components in regulating the slit diaphragm structure in human podocytes.

Silencing genes in the CME pathway led to defective endocytosis of slit diaphragm protein, disruption of the slit diaphragm structure and reduced nephrocyte function. These phenotypes are very similar to those we reported previously for nephrocytes in which Rab5, Rab11 or genes of the exocyst complex were silenced [35]. Based on those and our current results, we propose the following process of endocytosis and recycling for slit diaphragm proteins (Fig. 8). First, the slit diaphragm proteins on the cell membrane are recognized by the AP-2 complex or other adaptor proteins. Then the AP-2 complex recruits the clathrin complex and additional adaptors, like Lap, to assemble the clathrin coat. Protein Shi mediates fission of these endocytic pits from the cell membrane. The clathrin-coated vesicles are later disassembled by Aux and Hsc70-4, thereby releasing clathrin, AP-2, Lap and Shi for the next cycle of endocytosis. The uncoated vesicles then enter the early endosome, formation of which requires Rab5. Next the slit diaphragm protein cargo is sorted into the Rab11-dependent recycling endosome. Rab11 recruits the exocyst complex to tether the recycling endosome to cell membrane. Finally, the two membranes fuse and the slit diaphragm proteins recycle back to the cell membrane to facilitate assembly of the slit diaphragm structure. There are still important questions left to be addressed: What triggers endocytosis of slit diaphragm proteins? How are the slit diaphragm cargo proteins selectively sorted into the Rab11-dependent recycling endosome instead of other destinations?

Fig. 8
figure 8

Schematic representation of slit diaphragm protein endocytosis and recycling pathway. In the Drosophila nephrocyte, plasma membrane invaginates to form lacuna channels (LC). Slit diaphragm (SD) forms at the mouth of these invaginations. The left dashed box indicates the process of slit diaphragm protein endocytosis through the clathrin-mediated endocytosis (CME) pathway. The slit diaphragm proteins at the cell membrane are recognized by the AP-2 complex or other adaptor proteins. Then the AP-2 complex recruits the clathrin complex and like-AP180 (Lap) adaptor to assemble the clathrin coat. Subsequently Shibire (Shi) mediates fission of the endocytic pit from the cell membrane. The clathrin-coated vesicles are later disassembled by auxilin (Aux) and Hsc70-4, releasing AP-2, clathrin and Shi for the next cycle of endocytosis. The uncoated vesicles enter the Rab5-positive early endosome (EE). The slit diaphragm protein cargo is sorted into the Rab11-dependent recycling endosome (RE). Rab11 recruits the exocyst complex to tether the recycling endosome to the cell membrane. The two membranes fuse and the slit diaphragm proteins recycle back to the cell membrane to facilitate assembly of the slit diaphragm structure. The right dashed box indicates slit diaphragm protein endocytosis through the clathrin-independent endocytosis (CIE) pathway, the exact route of which is not clear, which is indicated by the question mark

Full size image

Interestingly, Clathrin (Clc)-silenced nephrocytes still had vesicles undergoing endocytosis, with many vesicles visible inside the cell (Fig. 6b). In addition, slit diaphragm proteins accumulated in the cytoplasm in nephrocytes with depleted CME pathway genes. If slit diaphragm proteins can be endocytosed only through the CME pathway, these proteins would have accumulated on the cell membrane, instead of being trapped in the cytoplasm. Therefore, we reason that a clathrin-independent endocytosis (CIE) pathway has to be present to transport slit diaphagm proteins into the cytoplasm when the CME pathway is blocked. Slit Diaphgam (SD) proteins could enter the cytoplasm through both CME and CIE, but can only be recycled back to the nephrocyte cell membrane through the CME pathway. Therefore, we speculate that a protein quality check process is present in the CME pathway (but not in the CIE pathway) to ensure non-damaged SD proteins to be recycled. We are currently investigating this protein quality check process in the CME pathway. We are also searching for the CIE pathway that is involved in slit diaphragm endocytosis, and try to identify its components and biological function.

Our results demonstrate that the Drosophila nephrocyte makes an excellent model system to identify genes involved in slit diaphragm protein endocytosis and recycling. In fact, the nephrocyte can also serve as an effective model to study general endocytosis and recycling. The human homologs for the CME genes which we identified in this study should be examined for possible mutations in patients with nephrotic syndrome. We could target the regulators in the CME pathway to improve the function of podocytes and find better treatment for patients with nephrotic syndrome. For example, Bis-T-23, a drug that increases the activity of dynamin, was able to ameliorate glomerular damage in multiple vertebrate models of kidney diseases [24]. The Drosophila nephrocyte model provides a versatile platform for identifying additional candidate genes in glomerular disease, to unravel the molecular mechanism of glomerular dysfunction and uncover potential pharmacological interventions for glomerular disease.

Materials and methods

Fly strains

All fly stocks were kept on standard food at 25 °C. Flies carrying Hand-GFP, Dot-Gal4, MHC-ANF-RFP transgenes have been described previously [36]. The Dot-Gal4 (#67608) and all UAS-RNAi transgenic RNAi lines were obtained from the Bloomington Drosophila stock center: UAS-shiRNAi (#28513), UAS-ChcRNAi (#27530), UAS-ClcRNAi (#27496), UAS-Arf79FRNAi (#66174), UAS-AP-2αRNAi (#27322), UAS-AP-2μRNAi (#28040), UAS-AP-2σRNAi (#27322), UAS-AP-1-2βRNAi (#28328), UAS-AP-1γRNAi (#27533), UAS-AP-1μRNAi (#27534), UAS-AP-1σRNAi (#40895), UAS-lapRNAi (#28358), UAS-auxRNAi (#28509), UAS-Hsc70-4RNAi (#28709), UAS-Rab5RNAi (#34832), UAS-Rab7RNAi (#27051), UAS-Vps29RNAi (#38963), UAS-Rab11RNAi (#42709).

ANF-RFP uptake assay

Briefly, 10 virgin female flies from the MHC-ANF-RFP, Hand-GFP and Dot-Gal4 transgenic lines were crossed to 5 male flies from UAS-RNAi transgenic lines at 25 °C. Pericardial nephrocytes of newly emerged adult flies (within 24 h of eclosion) were dissected and kept in Drosophila artificial hemolymph to assay RFP accumulation detected by fluorescence microscopy.

Dextran uptake assay

Flies carrying Hand-GFP and Dot-Gal4 transgenes were crossed with flies carrying the UAS-RNAi transgenes at 25 °C. Dextran uptake was assessed in adult flies one-day post-emergence by dissection of pericardial nephrocyte in Drosophila artificial hemolymph and examination of the cells by fluorescence microscopy after a 20 min incubation with Texas Red labeled Dextran (10 kD, 0.02 mg/ml).

Immunohistochemistry

Pericardial nephrocytes from one day post-emergence adult flies was dissected in Drosophila artificial hemolymph and then heat-fixed for 15 s in Drosophila artificial hemolymph preheated to 103 °C. Tissues were washed twice for 10 min with 1X PBST (1X phosphate buffered saline (PBS), with 0.1% TritonX-100). Tissues were then blocked with 2% BSA (Bovine Serum Albumin) in 1X PBST for 30 min. Samples were incubated with primary antibody overnight at 4 °C. Tissues were washed three times for 10 min with 1X PBST and then incubated with secondary antibody for 2 h at room temperature. Tissues were washed again thrice for 10 min with 1X PBST and mounted using Vectashield mounting media. The mouse monoclonal anti-Pyd antibody (PYD2) was obtained from Developmental Studies Hybridoma Bank. The rabbit anti-Sns antibody was a gift from Dr. Britta George [2]. The rabbit anti-Clc antibody was kindly provided by Dr. Graeme Davis [8]. The rabbit anti-Rab7 antibody was from Dr. Akira Nakamura. The Alexa Fluor-conjugated secondary antibodies were ordered from Thermo Fisher Scientific.

Confocal imaging

Confocal imaging was performed with a Zeiss LSM900 microscope using a 63x Plan-Apochromat 1.4 N.A. oil objective under Airyscan SR mode. For quantitative comparisons of fluorescence intensity, common settings were chosen to avoid oversaturation. ImageJ Software was used for image processing.

Transmission electron microscopy (TEM)

TEM was carried out using standard procedures. Briefly, one-day-old adult flies of the indicated genotypes were dissected in artificial hemolymph and fixed in 8% paraformaldehyde for 10 min. Then the tissues were further trimmed in 1X PBS. The trimmed samples were transferred into fixation buffer containing 4% paraformaldehyde and 2.5% glutaraldehyde. The samples were further processed and analyzed using a FEI Tecnai T12 TEM.

Statistics

Statistical analyses were performed with Graphpad Prism 7 software. For quantification of relative ANF-RFP or Dextran-linked Texas Red fluorescence, 20 nephrocytes were analyzed from each of 3 flies per indicated genotype. For quantification of the thickness of basement membrane, 10 measurements were analyzed in each genotype. Each data set is presented as mean ± SD. Results were analyzed by two-tailed Student’s t-test. P values < 0.05 were considered statistically significant (**P < 0.01; ***P < 0.001).

Availability of data and materials

All data and materials generated in this study are available publicly upon request.

References

  1. Chen MS, Obar RA, Schroeder CC, Austin TW, Poodry CA, Wadsworth SC, Vallee RB. Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis. Nature. 1991;351:583–6.

    Article  CAS  PubMed  Google Scholar 

  2. Dlugos CP, Picciotto C, Lepa C, Krakow M, Stöber A, Eddy ML, Weide T, Jeibmann A, Krahn MP, Van Marck V, Klingauf J, Ricker A, Wedlich-Soldner R, Pavenstadt H, Klambt C, George B. Nephrin signaling results in integrin β1 activation. J Am Soc Nephro. 2019;30(6):1006–19.

    Article  CAS  Google Scholar 

  3. Dorval G, Kuzmuk V, Gribouval O, Welsh GI, Bierzynska A, Schmitt A, Miserey-Lenkei S, Koziell A, Haq S, Benmerah A, Mollet G, Boyer O, Saleem MA, Antignac C. TBC1D8B loss-of-function mutations lead to X-linked nephrotic syndrome via defective trafficking pathways. Am J Hum Genet. 2019;104:348–55.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Doublier S, Ruotsalainen V, Salvidio G, Lupia E, Biancone L, Conaldi PG, Reponen P, Tryggvason K, Camussi G. Nephrin redistribution on podocytes is a potential mechanism for proteinuria in patients with primary acquired nephrotic syndrome. Am J Pathol. 2001;158:1723–31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Doublier S, Salvidio G, Lupia E, Ruotsalainen V, Verzola D, Deferrari G, Camussi G. Nephrin expression is reduced in human diabetic nephropathy: evidence for a distinct role for glycated albumin and angiotensin II. Diabetes. 2003;52:1023–30.

    Article  CAS  PubMed  Google Scholar 

  6. Fu Y, Zhu JY, Richman A, Zhao Z, Zhang F, Ray PE, Han Z. A Drosophila model system to assess the function of human monogenic podocyte mutations that cause nephrotic syndrome. Hum Mol Genet. 2017;26:768–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Gupta GD, Swetha MG, Kumari S, Lakshminarayan R, Dey G, Mayor S. Analysis of endocytic pathways in Drosophila cells reveals a conserved role for GBF1 in internalization via GEECs. PLoS ONE. 2009;4:e6768.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Heerssen H, Fetter RD, Davis GW. Clathrin dependence of synaptic-vesicle formation at the Drosophila neuromuscular junction. Curr Biol. 2008;18:401–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Hemalatha A, Mayor S. Recent advances in clathrin-independent endocytosis. F1000Res. 2019. https://doi.org/10.12688/f1000research.16549.1.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Hemalatha A, Prabhakara C, Mayor S. Endocytosis of Wingless via a dynamin-independent pathway is necessary for signaling in Drosophila wing discs. Proc Natl Acad Sci USA. 2016;113:E6993-7002.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Hermle T, Braun DA, Helmstadter M, Huber TB, Hildebrandt F. Modeling monogenic human nephrotic syndrome in the Drosophila garland cell nephrocyte. J Am Soc Nephrol. 2017;28:1521–33.

    Article  CAS  PubMed  Google Scholar 

  12. Hermle T, Schneider R, Schapiro D, Braun DA, van der Ven AT, Warejko JK, Daga A, Widmeier E, Nakayama M, Jobst-Schwan T, Majmundar AJ, Ashraf S, Rao J, Finn LS, Tasic V, Hernandez JD, Bagga A, Jalalah SM, el Desoky S, Kari JA, Laricchia KM, Lek M, Rehm HL, Macarthur DG, Mane S, Lifton RP, Shril S, Hildebrandt F. GAPVD1 and ANKFY1 mutations implicate RAB5 regulation in nephrotic syndrome. J Am Soc Nephrol. 2018;29:2123–38.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Jones N, Blasutig IM, Eremina V, Ruston JM, Bladt F, Li H, Huang H, Larose L, Li SS, Takano T, Quaggin SE, Pawson T. Nck adaptor proteins link nephrin to the actin cytoskeleton of kidney podocytes. Nature. 2006;440:818–23.

    Article  CAS  PubMed  Google Scholar 

  14. Kampf LL, Schneider R, Gerstner L, Thunauer R, Chen M, Helmstadter M, Amar A, Onuchic-Whitford AC, Loza Munarriz R, Berdeli A, Muller D, Schrezenmeier E, Budde K, Mane S, Laricchia KM, Rehm HL, Macarthur DG, Lifton RP, Walz G, Romer W, Bergmann C, Hildebrandt F, Hermle T. TBC1D8B mutations implicate RAB11-dependent vesicular trafficking in the pathogenesis of nephrotic syndrome. J Am Soc Nephrol. 2019;30:2338–53.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Kestila M, Lenkkeri U, Mannikko M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K. Positionally cloned gene for a novel glomerular protein–nephrin—is mutated in congenital nephrotic syndrome. Mol Cell. 1998;1:575–82.

    Article  CAS  PubMed  Google Scholar 

  16. Kumari S, Mayor S. ARF1 is directly involved in dynamin-independent endocytosis. Nat Cell Biol. 2008;10:30–41.

    Article  CAS  PubMed  Google Scholar 

  17. Martin CE, Jones N. Nephrin signaling in the podocyte: an updated view of signal regulation at the slit diaphragm and beyond. Front Endocrinol. 2018;9:302.

    Article  Google Scholar 

  18. Naslavsky N, Caplan S. The enigmatic endosome–sorting the ins and outs of endocytic trafficking. J Cell Sci. 2018. https://doi.org/10.1242/jcs.216499.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Nihalani D, Solanki AK, Arif E, Srivastava P, Rahman B, Zuo X, Dang Y, Fogelgren B, Fermin D, Gillies CE, Sampson MG, Lipschutz JH. Disruption of the exocyst induces podocyte loss and dysfunction. J Biol Chem. 2019;294:10104–19.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Owen DJ, Collins BM, Evans PR. Adaptors for clathrin coats: structure and function. Annu Rev Cell Dev Biol. 2004;20:153–91.

    Article  CAS  PubMed  Google Scholar 

  21. Pavenstadt H, Kriz W, Kretzler M. Cell biology of the glomerular podocyte. Physiol Rev. 2003;83:253–307.

    Article  CAS  PubMed  Google Scholar 

  22. Qin XS, Tsukaguchi H, Shono A, Yamamoto A, Kurihara H, Doi T. Phosphorylation of nephrin triggers its internalization by raft-mediated endocytosis. J Am Soc Nephrol. 2009;20:2534–45.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Quack I, Rump LC, Gerke P, Walther I, Vinke T, Vonend O, Grunwald T, Sellin L. beta-Arrestin2 mediates nephrin endocytosis and impairs slit diaphragm integrity. Proc Natl Acad Sci USA. 2006;103:14110–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Schiffer M, Teng B, Gu C, Shchedrina VA, Kasaikina M, Pham VA, Hanke N, Rong S, Gueler F, Schroder P, Tossidou I, Park JK, Staggs L, Haller H, Erschow S, Hilfiker-Kleiner D, Wei C, Chen C, Tardi N, Hakroush S, Selig MK, Vasilyev A, Merscher S, Reiser J, Sever S. Pharmacological targeting of actin-dependent dynamin oligomerization ameliorates chronic kidney disease in diverse animal models. Nat Med. 2015;21:601–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Soda K, Balkin DM, Ferguson SM, Paradise S, Milosevic I, Giovedi S, Volpicelli-Daley L, Tian X, Wu Y, Ma H, Son SH, Zheng R, Moeckel G, Cremona O, Holzman LB, de Camilli P, Ishibe S. Role of dynamin, synaptojanin, and endophilin in podocyte foot processes. J Clin Invest. 2012;122:4401–11.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Tryggvason K, Patrakka J, Wartiovaara J. Hereditary proteinuria syndromes and mechanisms of proteinuria. N Engl J Med. 2006;354:1387–401.

    Article  CAS  PubMed  Google Scholar 

  27. Unnersjo-Jess D, Scott L, Blom H, Brismar H. Super-resolution stimulated emission depletion imaging of slit diaphragm proteins in optically cleared kidney tissue. Kidney Int. 2016;89:243–7.

    Article  PubMed  Google Scholar 

  28. Unnersjo-Jess D, Scott L, Sevilla SZ, Patrakka J, Blom H, Brismar H. Confocal super-resolution imaging of the glomerular filtration barrier enabled by tissue expansion. Kidney Int. 2018;93:1008–13.

    Article  PubMed  Google Scholar 

  29. van der Bliek AM, Meyerowitz EM. Dynamin-like protein encoded by the Drosophila shibire gene associated with vesicular traffic. Nature. 1991;351:411–4.

    Article  PubMed  Google Scholar 

  30. Verma R, Kovari I, Soofi A, Nihalani D, Patrie K, Holzman LB. Nephrin ectodomain engagement results in Src kinase activation, nephrin phosphorylation, Nck recruitment, and actin polymerization. J Clin Invest. 2006;116:1346–59.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Verstreken P, Kjaerulff O, Lloyd TE, Atkinson R, Zhou Y, Meinertzhagen IA, Bellen HJ. Endophilin mutations block clathrin-mediated endocytosis but not neurotransmitter release. Cell. 2002;109:101–12.

    Article  CAS  PubMed  Google Scholar 

  32. Verstreken P, Koh TW, Schulze KL, Zhai RG, Hiesinger PR, Zhou Y, Mehta SQ, Cao Y, Roos J, Bellen HJ. Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron. 2003;40:733–48.

    Article  CAS  PubMed  Google Scholar 

  33. Waters AM, Wu MY, Huang YW, Liu GY, Holmyard D, Onay T, Jones N, Egan SE, Robinson LA, Piscione TD. Notch promotes dynamin-dependent endocytosis of nephrin. J Am Soc Nephrol. 2012;23:27–35.

    Article  CAS  PubMed  Google Scholar 

  34. Weavers H, Prieto-Sanchez S, Grawe F, Garcia-Lopez A, Artero R, Wilsch-Brauninger M, Ruiz-Gomez M, Skaer H, Denholm B. The insect nephrocyte is a podocyte-like cell with a filtration slit diaphragm. Nature. 2009;457:322–6.

    Article  CAS  PubMed  Google Scholar 

  35. Wen P, Zhang F, Fu Y, Zhu JY, Han Z. Exocyst genes are essential for recycling membrane proteins and maintaining slit diaphragm in drosophila nephrocytes. J Am Soc Nephrol. 2020. https://doi.org/10.1681/ASN.2019060591.

    Article  PubMed  PubMed Central  Google Scholar 

  36. Zhang F, Zhao Y, Han Z. An in vivo functional analysis system for renal gene discovery in Drosophila pericardial nephrocytes. J Am Soc Nephrol. 2013;24:191–7.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Zhuang S, Shao H, Guo F, Trimble R, Pearce E, Abmayr SM. Sns and Kirre, the Drosophila orthologs of Nephrin and Neph1, direct adhesion, fusion and formation of a slit diaphragm-like structure in insect nephrocytes. Development. 2009;136:2335–44.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We thank the Bloomington Drosophila Stock Center based at Indiana University for the Drosophila stocks. We thank Dr. Ru-ching Hsia at the Electron Microscopy Core Imaging Facility at University of Maryland, Baltimore for assistance with transmission electron microscopy (TEM).

Funding

This work was supported by National Institutes of Health grants R01DK098410 (Z.H.) and R01DK120908 (Z.H.). We also acknowledge the support of University of Maryland Baltimore, Institute for Clinical and Translational Research (ICTR) on its support on using the Electronic Microscopy core service.

Author information

Author notes

  1. Luyao Wang and Pei Wen contributed equally to this study

Authors and Affiliations

  1. Department of Nephrology, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe Eastern Road, Zhengzhou, 450052, Henan, China

    Luyao Wang & Zhanzheng Zhao

  2. Center for Precision Disease Modeling, Department of Medicine, University of Maryland School of Medicine, 670 West Baltimore Street, Baltimore, MD, 21201, USA

    Luyao Wang, Pei Wen, Joyce van de Leemput & Zhe Han

  3. Division of Endocrinology, Diabetes and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, 21201, USA

    Luyao Wang, Pei Wen, Joyce van de Leemput & Zhe Han

Authors
  1. Luyao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pei Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joyce van de Leemput
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhanzheng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhe Han
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

PW, ZZ. and ZH designed the study; LW and PWcarried out the experiments; LW, PW and ZH analyzed and interpreted the data; LW and PW prepared the figures; ZH and JL drafted and revised the manuscript; the manuscript has been critically reviewed and the final version approved by all authors. The authors declare that they have no competing interests. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Zhanzheng Zhao or Zhe Han.

Ethics declarations

Ethicss approval and consent to participate

Not applicable.

Consent for publication

The authors give our consent for publishing this work in Cell & Bioscience.

Competing interests

The authors declare no competing interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file1:

Table S1. The RNAi screen of endocytosis genes required for slit diaphragm protein endocytosis and recycling. Figure S1. The clathrin genes, Chc and Clc, are required for nephrocyte function. A MHC-ANF-RFP derived hemolymph ANF-RFP (red) uptake by nephrocytes. Hand-GFP labels both nephrocytes (big nucleus) and heart cells (small nucleus). Wild type (Control) flies accumulated abundant ANF-RFP. Subsequent panels show that Chc and Clc genes RNAi silencing severely reduced the ANF-RFP accumulation level. B Texas Red-labeled 10 kD Dextran particles uptake by nephrocytes. Particles were easily taken in and collected by wild type control nephrocytes in an ex vivo assay. Silencing of Chc and Clc genes clearly reduced levels of accumulated Dextran. C Quantification of relative ANF-RFP fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. D Quantification of relative Dextran-linked Texas Red fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. ***P<0.001. Figure S2. The clathrin genes, Chc and Clc, are required for slit diaphragm protein Sns endocytosis. A-A’ Sns (red) distributed uniformly and smoothly in parallel lines in fingerprint like pattern in control (wild type) nephrocytes. Scale bar: 1μm. B-C’ Silencing of Chc and Clc resulted in severe disruption of Sns cell surface localization. The Sns-positive lines became more curved and shorter, and many became dots. The lines were no longer parallel, and the spacing became irregular. Figure S3. The Drosophila dynamin homolog, Shi, is required for nephrocyte function. A MHC-ANF-RFP derived hemolymph ANF-RFP (red) uptake by nephrocytes. Hand-GFP labels both nephrocytes (big nucleus) and heart cells (small nucleus). Wild type (Control) flies accumulated abundant ANF-RFP. Subsequent panel shows that shi gene RNAi silencing severely reduced the ANF-RFP accumulation level. B Quantification of relative ANF-RFP fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. C Texas Red-labeled 10 kD Dextran particles uptake by nephrocytes. Particles were easily taken in and collected by wild type control nephrocytes in an ex vivo assay. Silencing shi clearly reduced levels of accumulated Dextran. D Quantification of relative Dextran-linked Texas Red fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. ***P<0.001. Figure S4. The Clc-IR transgene can specifically decrease Clc protein level. Anti-Clc antibody fluorescent staining of Clc expression (red) in nephrocytes. Left panel, in control nephrocytes, Clc is strongly expressed and localized mainly close to plasma membrane. Right panel, in clc-silenced nephrocytes, the protein level of Clc is below the detection limit, suggesting highly effective knockdown. Figure S5. AP-2 complex is required for nephrocyte function. A MHC-ANF-RFP derived hemolymph ANF-RFP (red) uptake by nephrocytes. Hand-GFP labels both nephrocytes (big nucleus) and heart cells (small nucleus). Wild type (Control) flies accumulated abundant ANF-RFP. Subsequent panels show that AP-2α, AP-2μ, AP-2Ω and AP-1-2β genes RNAi silencing severely reduced the ANF-RFP accumulation level. B Quantification of relative ANF-RFP fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. C Texas Red-labeled 10 kD Dextran particles uptake by nephrocytes. Particles were easily taken in and collected by wild type control nephrocytes in an ex vivo assay. Silencing of AP-2α, AP-2μ, AP-2Ω and AP-1-2β genes clearly reduced levels of accumulated Dextran. D Quantification of relative Dextran-linked Texas Red fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. ***P<0.001. Figure S6. AP-2 complex is required for Sns protein proper localization. A-A’ Sns (red) distributed uniformly and smoothly in parallel lines in finger-print like pattern in control (wild type) nephrocytes. Scale bar: 1μm. B-D Silencing of AP-1-2β, AP-2α, AP-2μ and AP-2σ resulted in severe disruption of Sns cell surface localization. Figure S7. AP-1 complex is not required for Pyd protein proper localization. A-A″ Sns (red) distributed uniformly and smoothly in parallel lines in finger-print like pattern in control (wild type) nephrocytes, with DAPI (blue nuclear stain). Scale bar: 1μm. B-D″ Silencing of AP-1γ, AP-1σ and AP-1μ showed similar pattern of Pyd cell surface localization as the control. Figure S8. The adaptor protein Lap and uncoating proteins, Aux and Hsc70-4, are required for nephrocyte function. A MHC-ANF-RFP derived hemolymph ANF-RFP (red) uptake by nephrocytes. Hand-GFP labels both nephrocytes (big nucleus) and heart cells (small nucleus). Wild type (Control) flies accumulated abundant ANF-RFP. Subsequent panels show that Lap, Aux and Hsc70-4 genes RNAi silencing severely reduced the ANF-RFP accumulation level. B Quantification of relative ANF-RFP fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. C Texas Red-labeled 10 kD Dextran particles uptake by nephrocytes. Particles were easily taken in and collected by wild type control nephrocytes in an ex vivo assay. Silencing of Lap, Aux and Hsc70-4 genes clearly reduced levels of accumulated Dextran. D Quantification of relative Dextran-linked Texas Red fluorescence in nephrocytes expressing the indicated gene silencing RNAi construct. ***P<0.001. Figure S9. The adaptor protein Lap and uncoating proteins, Aux and Hsc70-4, are required for Sns protein proper localization. A-A’ Sns (red) distributed uniformly and smoothly in parallel lines in fingerprint like pattern in control (wild type) nephrocytes. Scale bar: 1μm. B-D’ Silencing of Lap, Aux, Hsc70-4 resulted in severe disruption of Sns cell surface localization. Figure S10. The Rab7-IR transgene can specifically decrease Rab7 protein level. Anti-Rab7 antibody fluorescent staining of Rab7 expression (green) in nephrocytes, with DAPI (blue) nuclear stain. In control nephrocytes, Rab7 is strongly expressed. In rab7-silenced nephrocytes, the protein level of Rab7 is significantly reduced, suggesting highly effective knockdown.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, L., Wen, P., van de Leemput, J. et al. Slit diaphragm maintenance requires dynamic clathrin-mediated endocytosis facilitated by AP-2, Lap, Aux and Hsc70-4 in nephrocytes. Cell Biosci 11, 83 (2021). https://doi.org/10.1186/s13578-021-00595-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13578-021-00595-4

Keywords

Cell & Bioscience

ISSN: 2045-3701

Contact us