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Fig. 3 | Cell & Bioscience

Fig. 3

From: Slit diaphragm maintenance requires dynamic clathrin-mediated endocytosis facilitated by AP-2, Lap, Aux and Hsc70-4 in nephrocytes

Fig. 3

The AP-2 complex, a regulator of clathrin, is required for endocytosis of slit diaphragm protein Pyd. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. aa″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′a″). be Silencing of AP-1-2β (bb″), AP-2μ (cc″), AP-2α (dd″) and AP-2σ (ee″) resulted in severe disruption of Pyd cell surface localization. Revealing intracellular localization of aggregate-like structures, and cell-surface distribution marked by high-density aggregation areas

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