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Fig. 4 | Cell & Bioscience

Fig. 4

From: Slit diaphragm maintenance requires dynamic clathrin-mediated endocytosis facilitated by AP-2, Lap, Aux and Hsc70-4 in nephrocytes

Fig. 4

Clathrin accessory proteins for assembly (Lap) and disassembly (Aux; Hsc70-4) are required for endocytosis of slit diaphragm protein Pyd. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. aa″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′a″). bb″ Silencing of lap resulted in intracellular aggregation of Pyd proteins with partial disruption of Pyd cell surface localization. Note the fingerprint-like pattern remained largely intact, however gaps between adjacent Pyd-positive ridges appeared increased. cd Silencing of aux (cc″) and Hsc70-4 (dd″) resulted in severe disruption of Pyd cell surface localization. Revealing intracellular localization of aggregate-like structures, and cell-surface distribution marked by high-density aggregation areas

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