Fig. 7
Genetic analysis of the destination of endocytosed slit diaphragm proteins. Immunofluorescence labeling for Pyd (red), with DAPI (blue) nuclear stain of adult fly nephrocytes, visualized by confocal microscopy. Scale bar: 1 μm. a–a″ In wild type (Control) nephrocytes, Pyd was localized to the cell membrane (a) and exhibited a uniform and smoothly distributed fingerprint-like pattern on the cell surface (a′–a″). b–b″ and e–e″ Silencing of rab5 and rab11 resulted in intracellular aggregation of Pyd proteins and disruption of Pyd cell surface localization. Rab5 is a critical regulator of early endosome formation, while Rab11 is important for recycling endosome function. c–d Silencing of rab7 (c–c″) or vps29 (d–d″) did not disrupt Pyd cell surface localization. Rab7 is critical for late endosome maturation, and Vps29 is a component of the Retromer complex, which is required for early endosome to Golgi retrograde trafficking