Hypoxia induces calpain activity and degrades SMAD2 to attenuate TGFβ signaling in macrophages
© Cui et al. 2015
Received: 20 March 2015
Accepted: 12 June 2015
Published: 4 July 2015
Under inflammatory conditions or during tumor progression macrophages acquire distinct phenotypes, with factors of the microenvironment such as hypoxia and transforming growth factor β (TGFβ) shaping their functional plasticity. TGFβ is among the factors causing alternative macrophage activation, which contributes to tissue regeneration and thus, resolution of inflammation but may also provoke tumor progression. However, the signal crosstalk between TGFβ and hypoxia is ill defined.
Exposing human primary macrophages to TGFβ elicited a rapid SMAD2/SMAD3 phosphorylation. This early TGFβ-signaling remained unaffected by hypoxia. However, with prolonged exposure periods to TGFβ/hypoxia the expression of SMAD2 declined because of decreased protein stability. In parallel, hypoxia increased mRNA and protein amount of the calpain regulatory subunit, with the further notion that TGFβ/hypoxia elicited calpain activation. The dual specific proteasome/calpain inhibitor MG132 and the specific calpain inhibitor 1 rescued SMAD2 degradation, substantiating the ability of calpain to degrade SMAD2. Decreased SMAD2 expression reduced TGFβ transcriptional activity of its target genes thrombospondin 1, dystonin, and matrix metalloproteinase 2.
Hypoxia interferes with TGFβ signaling in macrophages by calpain-mediated proteolysis of the central signaling component SMAD2.
Macrophages are found throughout the body, where they contribute to tissue homeostasis and orchestrate innate as well as adaptive immune responses. They display a remarkable plasticity, which allows them to change their functional repertoire with regard to the environment they are facing. Numerous sub-phenotypes are described. Based on distinct functional roles and marker expression profiles an operative useful but over-simplifying nomenclature categorizes classical, i.e., pro-inflammatory and alternatively activated macrophages . An alternatively activated macrophage phenotype is elicited by interleukin-4 (IL-4), IL-10, or transforming growth factor ß (TGFß), which are produced by helper 2 or regulatory T cells, tumor cells, or macrophages themselves during late stage immune responses and tumor cells. Monocyte-derived macrophages and tissue resident macrophages are found in tumors. These tumor-associated macrophages are known to support cancer progression rather than mounting anti-cancer responses. This tumor-supporting phenotype is elicited by cytokines like TGFß .
TGFß controls proliferation, differentiation, and fosters fibrosis. TGFß binds to transforming growth factor receptor 2 (TGFΒR2), which recruits and phosphorylates transforming growth factor receptor 1 (TGFΒR1), thereby activating downstream signaling . SMAD proteins mediate canonical TGFß signaling, while the PI3K-AKT, MAPK pathway conveys non-canonical signals . The canonical pathway phosphorylates regulatory SMADs via receptor activation. In macrophages stimulation with TGFβ predominantly activates the regulatory SMAD2 and −3 but activation of SMAD1 and −5 was also observed . Phosphorylated SMAD2 and SMAD3 share high affinity for SMAD4 and show oligomerization, which is necessary for nuclear translocation and activation of transcription . SMAD7 is an inhibitory SMAD, which negatively controls this pathway.
TGFß plays a substantial role in tumor progression, acting both as a tumor suppressor and tumor promoter . Early in tumorigenesis, TGFß arrests the cell-cycle, which is SMAD-dependent. With tumor progression, tumor cells often harbor inactivating mutations in the TGFß pathway, allowing them to overcome these growth-inhibitory effects. In the tumor microenvironment, TGFß responsive cells secret cytokines and enzymes to promote EMT (epithelial mesenchymal transition), angiogenesis, tumor invasion, and metastasis. Besides, TGFß also recruits and activates immune cells . Of particular interest is the induction of an alternatively activated macrophage phenotype, which promotes tumor development [8, 9]. TGFß-recruited macrophages are highly phagocytic for aberrant cells and thereby decrease the ability of tumor antigens to be delivered to the adaptive immune system . Inhibition of NF-ĸB in TGFß-polarized macrophages accounts for their reduced ability to produce pro-inflammatory cytokines [7, 11].
At sites of inflammation, like in tumors, macrophages face hypoxia. Hypoxia promotes angiogenesis, fibrosis, and immune suppression . Activation of the hypoxia-inducible factor (HIF) conveys many responses to hypoxia, by altering transcriptional outputs. HIF is a heterodimeric transcription factor composed of an ubiquitously expressed β-subunit and an oxygen-sensitive α-subunit [13, 14]. Three HIF-α subunits are identified in the human genome (HIF-1α, HIF-2α or EPAS1, and HIF-3α), which all share the oxygen-dependent degradation domain (ODD). In well-oxygenated tissue specific prolyl hydroxylases hydroxylate the ODD of the α-subunit, allowing their recognition by the von Hippel-Lindau tumor suppressor and subsequent proteosomal degradation [13, 14]. Under hypoxic conditions HIF-α is stabilized, translocates to the nucleus and thus, promotes transcription of target genes to induce angiogenesis, ensures cell survival, restores oxygen homeostasis but also promotes fibrosis . These functions overlap with TGFß-signaling, indicating some signal crosstalk [6, 12, 16]. In renal epithelial cells and prostate cancer cells TGFß provokes HIF-1α accumulation, which in turn enhances expression of common target genes [17, 18]. In contrast, TGFß alone failed to accumulate HIF-1α in macrophages  even though the prolyl-hydroxylase-2 and −3 (PHD2/3) mRNA were decreased . Although macrophages play an important role in fibrosis and immune suppression the impact of hypoxia on TGFß-signaling in macrophages is poorly understood. We provide evidence that TGFß-signaling under hypoxia is impaired, due to calpain-mediated SMAD2 degradation in macrophages.
Hypoxia attenuates TGFß-induced SMAD2 activation in macrophages
A decrease in SMAD2 under hypoxia is unrelated to TGFΒR2 or SMAD7
To determine the impact of hypoxia on TGFΒR2 protein expression, we examined its surface appearance after 8 h by flow cytometry (Fig. 2c, d). TGFΒR2 expression was neither altered by TGFß nor by the combination of hypoxia/TGFß, indicating that the SMAD2 decrease is unrelated to TGFΒR2 expression. In addition, Western blot analysis verified that the expression of SMAD7 increased by TGFß at 15 to 30 min, decreased after 1–2 h and remained low up to an 8 h incubation period. However, SMAD7 protein expression was similarly affected by TGFß supplied under normoxia and hypoxia (Fig. 2e, f). Thus, it appears that neither the expression of TGFBR2 nor of SMAD7 account for the specific effects of hypoxia seen at the level of SMAD2.
SMAD2 degradation under hypoxia is facilitated by calpain
In human macrophages, in contrast to epithelial and parenchymal cells [17, 18], periods of 8 h hypoxia or longer reduced TGFß-induced SMAD2 activation. Decreased phosphorylation of SMAD3, but not SMAD2, under hypoxia was noticed in HeLa cells and attributed to protein phosphatase 2A (PP2A) directly interacting with SMAD3 . In macrophages the decrease of p-SMAD2 was not due to PP2A activation, altered TGFBR2 receptor activation, or inhibitory SMAD7 expression, but rather resulted from hypoxia-evoked destabilization of SMAD2. Regulation of SMAD2 degradation is complex. Smurf2 is an E3 ubiquitin ligase, directly interacting with activated SMAD2 and promoting its proteasomal degradation. Neural precursor cell expressed developmentally down-regulated protein 4–2 (NEDD4-2), another E3 ubiquitin ligase like Smurf1 or 2, binds SMAD2 and SMAD3 but exclusively degrades SMAD2 . In addition, TGFβ-induced factor homeobox 1 (TGIF) interacting ubiquitin ligase 1 (Tiul1), induces SMAD2 to bind TGIF, which triggers SMAD2 degradation . Our data add proteolysis by the calpain system also to account for alterations in the SMAD2 protein amount. Interestingly, Lo and coworkers studied the proteasomal degradation of SMAD2 . Along our observations, their results suggested that lactacystin cannot rescue a SMAD2 decrease to the same extent as MG132, although they did not consider the calpain system.
The calpain system consists of a catalytic subunit (μ-calpain/m-calpain) and a common regulatory subnunit CAPNS1 as well as the inhibitory subunit calpastatin. After calcium binding, calpastatin detaches from the protein complex and calpain is activated by autolysis . The calpain system plays important roles in macrophages. IFNγ induces calpain mRNA and protein expression in U937 and THP1 cells . Calpain cleaves iNOS and thus, affects inflammatory responses . There are several reports that calpain activity is upregulated under hypoxia, with platelets being one typical example [32–35]. Hypoxia-induced thrombogenesis is associated with CAPNS1-dependent calpain activation in the platelet activation cascade . In line, we noticed increased calpain activity under TGFβ/hypoxia by upregulating CAPNS1 in macrophages. The calpain regulatory subunit functions like a chaperone, assisting the catalytic subunit to fold properly . We showed that combining TGFß and hypoxia is necessary to activate calpain in human macrophages as indicated in Fig. 4j, while hypoxia alone did not activate calpain. This is in line with findings showing that ischemia/hypoxia provokes an influx of Ca2+ into the cell [32, 35], but the Ca2+ increase is not sufficient to activate calpain [29, 37]. It is well established that TGFß could also increase the influx of Ca2+ into cells . Presumably, a high expression of CAPNS1 and the influx of Ca2+ by the combined stimulation, hypoxia plus TGFß, promotes activation of calpain. Calpain digests proteins at a limited number of cleavage sites producing large polypeptide fragments rather than small peptides or amino acid . Early studies suggested that preferred residues of the cleavage P2 position are Leu, Thr, and Val, and in the P1 position are Lys, Thr, and Arg . Following the P2-P1 rule, SMAD2 has 17 potential cleavage sites. In addition, calpain prefers small hydrophilic Ser residue at the P1’ position. Considering this preference, there are still 4 possible cleavage sites at K46, Y101, R108, Y266 for calpain in SMAD2. On the other hand, there are indications that the specificity for calpain is not exclusively governed by amino acid but rather by the 3D conformation of the substrates [29, 39]. Using currently available online tools at http://calpain.org for calpain substrates, amino acids at sites 166, 395, and 6 are the top 3 predicted cleavage sites with a score of 0.39, 0.39, 0.37 respectively . For the identification of the accurate cleavage sites more experiments are needed that would be beyond the scope of this work.
Our experimental conditions aimed to simulate conditions macrophages are facing when entering the tumor microenvironment, facing hypoxia and TGFß . SMAD2 and SMAD3 have distinct transcriptional roles in the tumor [27, 42, 43]. SMAD2 provokes secretion of anti-angiogenetic factors, such as thrombospondin 1 (TSP1, Fig. 5) as well as the VEGF-A antagonist sFlt-1, whereas SMAD3 induces the secretion of pro-angiogenetic factor, i.e., VEGF-A or plasminogen activator inhibitor 1 (PAI1, Fig. 5), promoting angiogenesis when SMAD2 is inactive [26, 44]. In addition, TSP1 overexpression in tumor cells enhances the recruitment of pro-inflammatory macrophages and reduces tumor growth . Conditional medium of SMAD2 knockdown fibroblast induces proliferation and in vivo deletion of SMAD2 in tumor cells generates a more aggressive phenotype compared to controls . The decrease of SMAD2 under TGFβ/hypoxia in tumor-associated macrophages may add to their roles supporting angiogenesis and tumor progression.
Angiogenesis influences tumor growth but also enables metastasis . Metastasis is a complex process requiring invasive growth of tumor cells, epithelial to mesenchymal transition (EMT), induction of angiogenesis, intra- and extravasation, and tumor growth at the secondary sites . Matrix metalloproteases (MMPs) like MMP2 coordinate multiple steps of this process. In general they promote metastasis by allowing extracellular matrix remodeling and releasing growth factors like VEGF from the matrix. MMP2 was also implicated in endothelial cell apoptosis [46, 48]. As full activation of MMP2 by the membrane type (MT)1-MMP provokes endothelial cell death and expression of MMP2 in endothelial cells and macrophages is reduced in acute hypoxia (Fig. 5d), one might speculate that this reduction prevents endothelial cell death under hypoxia when angiogenesis is usually enhanced [48, 49]. Other TGFß-induced genes involved in metastasis are enhanced under hypoxia since SMAD3 target genes like PAI-1 or CST6 (Fig. 5) are regulated by the hypoxia-inducible factor (HIF) as well [16, 50]. In a previous study we used ChIP-seq technology to identify HIF-1 and HIF-2 binding sites in primary human macrophages and identified, amongst others, CST6 and PAI-1 . Apparently, in these cases TGFß/hypoxia enhances rather than decreases gene expression in macrophages. Thus, specifically attenuating SMAD2 under TGFß/hypoxia might have functional roles on macrophages biology and tumor progression.
Material and methods
Cell culture and incubation procedures
If not indicated otherwise, all chemicals were purchased from Sigma (Steinheim, Germany), while cell lines came from ATCC (LGC Promochem, Wesel, Germany). J774A.1 cells were cultured in Dulbecco’s Modified Eagle Medium with 10 % fetal calf serum, 100 U/ml penicillin, and 100 μg/ml streptomycin (PAA Laboratories, Cölbe, Germany). Primary human monocytes were isolated from 50 ml buffy coats (DRK Blutspendedienst Baden-Württemberg-Hessen, Frankfurt, Germany). Blood was layered on a Ficoll-Isopaque gradient (P = 1077 g ml−1). The interphase containing peripheral blood mononuclear cells was obtained after centrifugation (800 × g, 20 min). Cells were recovered, washed twice in PBS, and left to adhere on culture dishes (Sarstedt, Nümbrecht, Germany) for 90 min at 37 °C. Non-adherent cells were removed. The medium was changed to fresh RPMI 1640 medium, supplemented with 3 % (v/v) human plasma (HP), 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (PAA Laboratories, Cölbe, Germany). Cells were kept at 37 °C in a humidified atmosphere with 5 % CO2. Monocytes were differentiated with human plasma for 6–8 days before they were treated with 10 ng/ml TGFß. Purity of macrophage cultures was about 90 %. To establish hypoxic conditions cells were incubated in a hypoxic workstation with 1 % O2, 94 % N2, 5 % CO2 (Invivo2 400, Ruskinn Technology, Leeds U.K.). Protein stability was assessed by stimulating macrophage with TGFß for 4 h, followed by 10 μg/mL cycloheximide (CHX). Samples were harvested after additional 1, 2, 3, or 4 h. Degradation of SMAD2 was followed by the addition of 10 μM lactacystin, 10 μM MG132 (Z-Leu-Leu-Leu-al), or 50 μM bafilomycin A1. After 2 h incubations with TGFß co-incubations with TGFß and inhibitors went on for 6 h. Phosphatase PP2A was inhibited by 10 μM okadaic acid (OA), preincubated for 1 h. 10 μM calpain inhibitor 1 was added simultaneously together with TGFß to macrophages.
Transfection of primary human macrophages
For siRNA transfections human macrophages were seeded in 6-well plates as described above. 50 nM siRNA against HIF-1α (ON-TARGETplus SMART pool, Human HIF1A, Thermo Scientific, Karlsruhe, Germany), HIF-2α (ON-TARGETplus SMART pool, Human EPAS1, Thermo Scientific), or non-targeting siRNA pool (GE Healthcare Dharmacon) was used with 16.8 μl HiPerfect (Qiagen, Hilden, Germany) in 500 μl medium with penicillin and streptomycin for each well. After adding the mix, cells were incubated for 24 h. Then medium was removed and replaced by medium containing penicillin, streptomycin and human serum to start experiments.
Western blot analysis
Cells were lysed in lysis-buffer (6.65 M urea, 10 % glycerol, 1 % SDS, 10 mM Tris/HCl pH 6.8, pH was adjusted to 7.4) and sonicated. After centrifugation (16.000 × g, 10 min) the protein content in the supernatants was determined by a protein assay kit (Bio-Rad, Munich, Germany), 70 μg total protein was separated on 10 % SDS gels and blotted on nitrocellulose membranes. The following antibodies were used: human pSMAD2 (#3108, Cell Signaling Technology), human pSMAD3 (ab52903, Abcam), human SMAD7 (ab124890 Abcam), human SMAD2/3 (#3102 Cell Signaling Technology), human calpain regulatory subunit (sc-32785, Santa Cruz), CAPN1 (C5736, Sigma), nucleolin (sc-13057, Santa Cruz), and ß-tubulin (T4026, Sigma). Horseradish peroxidase-conjugated goat anti-rabbit or anti-mouse IgG (Sigma) were used as secondary antibodies. Densitometry was performed using image J software (http://rsbweb.nih.gov/ij/). Briefly, rectangles were used to assess signal strength of each individual band and the background was measured by using the baseline tool and subtracted from individual values. Target protein expression was normalized to the loading control i.e., tubulin or nucleolin.
Luciferase reporter activity
The SBE4-Luc reporter plasmid was a gift from Bert Vogelstein (Addgene plasmid # 16495) , while the renilla control vector pRL-SV40 was bought from Promega (Frankfurt, Germany). J774A.1 cells were transiently transfected with 0.2 μg plasmid using JetPrime™ transfection reagent (Polyplus transfection, Illkirch, France) according to the manufacturer's protocol. After 16 h, medium was changed and cells were starved for 2 h prior to stimulation. Afterwards, cells were lysed and firefly as well as renilla luciferase activities were determined using a Dual Luciferase kit assay (Promega) on a Mithras LB 940 luminometer (Berthold, Bad Wildbad, Germany).
Flow cytometry (FACS)
For TGFΒR2 surface expression, single cell suspensions were generated from detached macrophages by digestion with accutase (PAA) for 10 min at 37 °C. 105 cells were transferred to new tubes. Before staining, non-specific antibody binding to Fcγ receptors was blocked with Fcγ Block Receptor Binding Inhibitor (eBioscience, Frankfurt, Germany) for 15 min on ice, followed by staining with human TGFΒR2 fluorescein-conjugated antibody (FAB241F,R&D Systems, Germany) using standard protocols. We used an LSRFortessa Cell Analyzer (BD, US) and results were analyzed using FlowJo software 7.6.1 (Treestar, Ashland, OR, USA). To discriminate macrophages from other non-leukocyte cells, samples were stained with CD45 (BD Bioscience). For daily instrument calibration we used Cytometer Setup and Tracking beads (BD Biosciences).
mRNA isolation and quantitative PCR
SMAD2, forward: CCGACACACCGAGATCCTAAC,
SMAD7, forward: CCAGGCTCCAGAAGAAGTTG,
TGFΒR2, forward: GGAAACTTGACTGCACCGTT,
CAPNS1, forward: GACACCCTGATCTGAAGACTGA,
CAPN1, forward: ACATGGAGGCCATCACTTTC
TSP1, forward: CCAGATCAGGCAGACACAGA
DST, forward: GATGCAGATCCGAAAACCCCT
MMP2, forward: AGGGCACATCCTATGACAGC
CST6, forward: CTACTTCCGAGACACGCACA
PAI1, forward: AGCTCCTTGTACAGATGCCG
TBP, forward: GGGCCGCCGGCTGTTTAACT,
Experiments for mRNA expression and flow cytometry were performed in duplicate and repeated at least 3 times. Western blot analysis was performed at least 3 times. Data are expressed as means ± SEM. Multigroup statistically significant differences were calculated after analysis of one-way variance (ANOVA) and Bonferroni’s test. A statistically significant difference between two groups was calculated by paired T test. Individual P values are given in the figures (*P < 0.05, **P < 0.01, ***P < 0.001).
This work was supported by the Deutsche Forschungsgemeinshaft (SFB 815, project A8), Deutsche Krebshilfe (111578), and Sander Foundation (2013.036.1).
Thanks to China Scholarship Council granting a stipend to Wei Cui.
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