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The longevity of tor1Δ, sch9Δ, and ras2Δ mutants depends on actin dynamics in Saccharomyces cerevisiae

Cell & Bioscience volume 5, Article number: 18 (2015) Cite this article

Abstract

Recent studies have revealed the role of actin dynamics in the regulation of yeast aging. Although the target of rapamycin (TOR) complex, serine/threonine kinase Sch9, and Ras2 have been shown to play important roles in aging for a long time, the relationship between these regulators and actin has not yet been reported. In this study we investigated the roles of actin polarization in tor1Δ, sch9Δ, and ras2Δ mutant cells. We found that the actin structures in tor1Δ, sch9Δ, and ras2Δ mutant cells were more dynamic than those in the wild type. Destruction of the actin structures with jasplakinolide decreased the life span of tor1Δ, sch9Δ, and ras2Δ mutants. Furthermore, deletion of SLA1 in tor1Δ, sch9Δ, and ras2Δ mutants inhibited the actin dynamics and life span. In addition, we found that the actin cytoskeleton of the long-lived mutant sch9Δ, depended on the transcription factors RIM15 and MSN2/4, but not GIS1, while the actin skeleton of the tor1Δ and ras2Δ mutants depended on RIM15 as expected. Our data suggest that the longevity of tor1Δ, sch9Δ, and ras2Δ mutants is dependent on actin dynamics.

Introduction

The budding yeast, Saccharomyces cerevisiae, has been successfully established as a model to study the mechanisms of aging. Currently, there are two models to study aging in yeast: the replicative life span (RLS), which represents the replicative capacity of a mother cell and is measured by counting the number of daughter cells produced by a mother cell [1,2], and the chronological life span (CLS), which is defined as the length of time the mother cell can survive in liquid medium [3,2].

Tor1 is a PIK-related protein kinase and one of the subunits of TOR complex 1 (TORC1). The TOR complex controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport, and autophagy, which are important cellular responses implicated in increased life span [4]. Previous studies suggest that the TOR pathway controls mitochondrial respiration and production of reactive oxygen species (ROS) in yeast, which is thought to be an important determinant for life span in yeast cells [5-7].

The yeast kinase Sch9 can mediate glucose-dependent signaling, stimulate growth and cellular proliferation, and decrease stress resistance [8,9]. The COOH-terminal region of Sch9 is highly homologous to the AGC family members of serine/threonine kinases, which include Akt/PKB and S6K in mammalian cells. Sch9 can be phosphorylated directly by TOR1 at multiple sites and by PDK1 in the activation loop [10]. Major scientific discoveries into the regulation of longevity by Sch9 in yeast comes from Longo’s Lab [11]. They have shown that sch9Δ cells can survive three times longer than wild type cells. Stress-resistance transcription factors, such as Msn2/4 and protein kinase, Rim15, are required for this life span extension [12].

RAS2 is a homolog of the mammalian oncogene, RAS, and is highly related to the RAS1 gene in budding yeast [3]. It codes for a small GTP-binding protein and has been shown to regulate the nitrogen starvation response through its effects on adenylate cyclase (encoded by the CYR1 gene). The GTP-bound form, Ras2, directly induces the production of cAMP by adenylate cyclase [10].

Previous studies have shown that actin dynamics regulates longevity though its underlying mechanism is still unclear [13,14]. It was suggested that decreased actin dynamics led to depolarization of the mitochondria and induced production of reactive oxygen species (ROS), which reduced cell longevity. Tor1, Sch9 and Ras2 are important regulators of aging, however, whether actin dynamics is required for longevity of tor1Δ, sch9Δ, and ras2Δ mutants is unknown.

In this study, the role of actin dynamics in the life span of tor1Δ, sch9Δ, and ras2Δ mutant yeast cells was investigated. Using genetic and biochemistry assays, we found that the actin pattern is more dynamic in tor1Δ, sch9Δ, and ras2Δ mutant cells than that in wild type cells, and that reduced actin dynamics, through either the actin stabilizing drug, jasplakinolide, or SLA1 deletion, led to shorter life spans in these mutants. Although actin dynamics is required for all three mutants, the actin cytoskeleton in the long-lived mutant sch9Δ, depended on the transcription factors, RIM15 and MSN2/4, while tor1Δ and ras2Δ depended on RIM15. These data suggest that the regulation of actin dynamics in tor1Δ, sch9Δ, and ras2Δ is based on different mechanisms.

Results

The actin structure of tor1Δ, sch9Δ, and ras2Δ mutants is more dynamic than that of wild type cells

In order to test if the tor1Δ, sch9Δ, and ras2Δ strains used in this study have similar extended life spans as in the previous study, the same medium was used to examine the chronological life span as noted in the previous study [11]. As expected, tor1Δ, sch9Δ, and ras2Δ was smaller in size than the DBY746 wild type cells (data not shown). At day 8 after inoculation, the viability of DBY746 was less than 5%. The viability of tor1Δ, sch9Δ, and ras2Δ cells at the same time points, however, was 73%, 85%, and 82%, respectively. At day 11, almost all the wild type cells were dead, but the viability of tor1Δ, sch9Δ, and ras2Δ cells was 38%,78%, and 73%, respectively. These data suggest that the yeast mutants tor1Δ, sch9Δ, and ras2Δ have longer life spans, as reported previously [12].

It was reported that increasing actin dynamics led to a longer life span [13]. Although a lot of studies have shown that Tor1, Sch9, and Ras2 are required for longevity, the actin dynamics in tor1Δ, sch9Δ, and ras2Δ cells were not examined. The dynamic actin structures in the wild type and mutant cells in Figure 1A were quantified. As shown in Figure 1B, the percentage of actin clumps in wild type cells decreased faster than that of tor1Δ, sch9Δ, and ras2Δ cells. On day 1, the percentage of actin clumps was about 62%, while in the tor1Δ, sch9Δ, and ras2Δ mutants, it is more than 78%. On day 5, the percentage of actin clumps is about 8%, while in tor1Δ, sch9Δ, and ras2Δ mutants, it is more than 42%. These results suggest that tor1Δ, sch9Δ, and ras2Δ have more dynamic actin structures compared to wild type cells. A previous study showed that cells with poor actin dynamics were resistant to the actin disrupting drug latrunculin-A (Lat-A) [15]. To test whether the actin structures in tor1Δ, sch9Δ, and ras2Δ are dynamic, we treated the wild type and tor1Δ, sch9Δ, and ras2Δ mutants with Lat-A, which destroys the actin structures; sla1Δ cells were used as the negative control. After 5 min of 50 μM of Lat-A treatment, the percentage of cells with actin patches in wild type, tor1∆, ras2∆, sch9∆ and sla1∆ is 54.7%, 79.6%, 82.8%, 84.8%, and 94.2%, respectively. After 10 min of 50 μM of Lat-A treatment, however, the percentage of cells with actin patches in wild type, ras2∆, tor1∆, sch9∆ and sla1∆ is 9.8%, 16.3%, 12.3%, 20.1%, and 85.5%, respectively. These data suggest that the actin structures in longevity cells are dynamic.

Figure 1
figure 1

The actin structure is more dynamic in longevity mutants. (A) Wild type (DBY746), tor1Δ (LY101), sch9Δ (LY102), and ras2Δ (LY103) cells were cultured in aging medium. Triplicate cultures for each strain were used and experiments were repeated three times. Error bars represent standard errors. (B) The actin dynamic pattern was examined on day 1, 3, and 5 for yeast strains in (A). *,p < 0.01. (C) Actin is dynamic in DBY746, tor1, sch9Δ, and ras2Δ. The cells were treated with 50 μM latrunculin A, and after 5 and 10 min the actin pattern was examined by fluorescent microscopy. Scale bar, 5 μm. (D) The actin dynamic pattern was quantified for yeast strains in (C).

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Jasplakinolide treatment decreases the actin dynamics and life span of tor1Δ, sch9Δ, and ras2Δ mutants

Jasplakinolide is an actin-stabilizing chemical and can block actin dynamics [16]. To further test whether actin dynamics is involved in the longevity of tor1Δ, sch9Δ, and ras2Δ mutants, the wild type, tor1Δ, sch9Δ, and ras2Δ mutant cells were treated with 2 μM jasplakinolide. As shown in Figure 2A and B, under the normal growth condition, the percentage of cells with actin patches in wild type, ras2∆, tor1∆, sch9∆ and sla1∆ is 64.7%, 89.6%, 94.8%, and 91.8%, respectively. After jasplakinolide treatment, however, the percentage of cells with actin patches in wild type, ras2∆, tor1∆ and sch9∆ is 12.5%, 14.7%, 27.2%, and 30.6%, respectively. These data suggest that the actin filaments was stabilized in wild type, tor1∆, sch9∆, and ras2∆ after jasplakinolide treatment. Since jasplakinolide interrupts actin dynamics, we wanted to investigate whether cell life span would decrease in mutants treated with jasplakinolide. As shown in Figure 2B, jasplakinolide treatment drastically decreased the life span of tor1Δ, sch9Δ, and ras2Δ mutants, but not the wild type cells. On day 11, without treatment, tor1Δ, sch9Δ, and ras2Δ mutant viability were 62%, 60%, and 64%, respectively. However, with jasplakinolide treatment, the viability of these mutants decreased to 2%, 8%, and 7%, respectively. These data suggest that a dynamic actin structure is required for longevity of tor1Δ, sch9Δ, and ras2Δ mutants.

Figure 2
figure 2

Dysfunction of actin dynamics after jasplakinolide treatment leads to reduced actin dynamics and reduced chronological life span in DBY746, tor1Δ, sch9Δ, and ras2Δ yeast cells. (A) 2 μM of jasplakinolide was added into the medium. The actin dynamic pattern was examined by fluorescent microscopy. Scale bar, 5 μm. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to growth medium to a final concentration of 3.7%. After washing with 1× PBS solution for three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h and images were taken by fluorescent microscope. (B) The actin dynamic pattern was quantified for yeast strains in (A). (C) Chronological life span in DBY746, tor1Δ (LY101), sch9Δ (LY102), and ras2Δ (LY103) cells decreased after jasplakinolide treatment.

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The deletion of SLA1 in tor1Δ, sch9Δ, and ras2Δ mutants inhibits actin dynamics and life span

Sla1 is associated with actin patches and cables, the two main forms of actin structures. It was reported that Sla1 is required for dynamic actin organization [13]. To further confirm the role of actin dynamics in the longevity of tor1Δ, sch9Δ, and ras2Δ mutants, SLA1 was deleted in these mutants. As shown in Figure 3A, upon deletion of SLA1, actin dynamics was drastically inhibited in both wild type and mutant cells. We also measured the life span of single mutant tor1Δ, sch9Δ, and ras2Δ cells and double mutant tor1Δ sla1∆, sch9Δ sla1∆ and ras2Δ sla1∆ cells. As expected, Sla1 is required for longevity of tor1Δ, sch9Δ, and ras2Δ (Figure 3B). Taken together, actin dynamics controls the longevity of tor1Δ, sch9Δ, and ras2Δ cells.

Figure 3
figure 3

SLA1 is required for actin dynamics and chronological life span in DBY746, tor1, sch9Δ, and ras2Δ cells. (A) tor1Δ sla1Δ (LY113), sch9Δ sla1Δ (LY114), and ras2Δ sla1Δ (LY115) showed less actin dynamics than tor1Δ (LY101), sch9Δ (LY102), and ras2Δ (LY103) single mutants. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to growth medium to final concentration of 3.7%. After washing with 1× PBS solution for three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h and images were taken by fluorescent microscope. Scale bar, 5 μm. (B) tor1Δsla1Δ (LY113), sch9Δsla1Δ (LY114), and ras2Δsla1Δ (LY115) double mutants have decreased life spans compared to single mutants. Sla1 is associated with actin patches and cables, which organizes the yeast actin cytoskeleton.

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The actin cytoskeleton dynamics in sch9Δ mutant cells depends on Rim15 and Msn2/4, while it relies on Rim15 in tor1∆ and ras2∆ mutants

RIM15, GIS1, and MSN2/4 are effectors of Tor1, Sch9, and Ras2, which are also shown to regulate life span. Previous studies showed that GIS1, RIM15, and MSN2/4 are required for the longevity of sch9∆ mutants [12], while RIM15 is required for the longevity of tor1∆,and Rim15 and Gis1 is required for the longevity of ras2∆ mutant. Because actin dynamics controls the life span of tor1Δ, sch9Δ, and ras2Δ mutants, we speculate that their effectors may also regulate the actin dynamics in these mutants. To test this hypothesis, we mutated RIM15, GIS1, or MSN2/4 in sch9Δ, RIM15 or GIS1 in ras2∆, and RIM15 in tor1∆ mutant. The actin dynamics in these cells were then examined by fluorescent microscopy. As shown in Figure 4A, at day 6, the actin dynamics decreased drastically in the wild type, sch9∆rim15∆, and sch9∆msn2/4∆ cells. However, there was no difference between sch9∆ and sch9∆gis1∆ cells. These data indicate RIM15 and MSN2/4, but not GIS1 is required for actin dynamics in sch9∆ cells. Actin organization was also examined in wild type, tor1Δ, tor1Δrim15∆, ras2Δ, gis1∆, ras2∆gis1Δ and ras2Δrim15∆ cells. As shown in Figure 4B and C, the actin structure is dynamic in tor1Δ, ras2Δ and ras2∆gis1Δ, but not in wild type and gis1Δ cells. However, the actin was not dynamic in tor1Δrim15∆ and ras2Δrim15∆ double mutants. These data suggests that actin cytoskeleton dynamics in sch9Δ cells depends on its effectors RIM15 and MSN2/4, and in tor1∆ and ras2∆, depends on RIM15.

Figure 4
figure 4

The dynamic actin pattern in sch9Δ, tor1Δ, and ras2Δ cells relies on different effectors. The actin cytoskeleton in sch9Δ depends on the RIM15 and MSN2/4 transcription factors, not GIS1 (A), while tor1Δ and ras2Δ depend on the RIM15 (B, C). Wild type and mutant strains were grown in aging medium. At indicated time points, cells were removed either for testing of cell viability or for fluorescence microscopy. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to the growth medium to a final concentration of 3.7%. After washing with 1× PBS solution for three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h and images were taken by fluorescent microscope. Scale bar, 5 μm.

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Discussion

A recent study showed that Tor1, Sch9, and Ras2 can regulate carbon source substitution and control calorie restriction in life span extension [17]. Although actin has long been proposed to play an important role in aging [13], whether actin dynamics is involved in the increased life span of tor1∆, sch9Δ, and ras2∆ mutants has never been examined. To answer this question, we examined actin organization in the wild type, tor1∆, sch9Δ, and ras2∆ mutants by fluorescence microscopy. Consistent with previous reports, the actin structure is dynamic in these longevity mutants but not wild type cells. Numerous bright small separate dots around the cell were found in tor1∆, sch9Δ, and ras2∆ mutant cells. To the contrary, actin in the DBY746 wild type looks like a large aggregate clump after day 3 or 5 cultures. The aggregates were shown to be extremely stable structures and less dynamic [16], but the separate smaller dots were not. To further confirm the role of actin dynamics in the aging process, the actin structure was destroyed either by jasplakinolide treatment or through deletion of the SLA1 gene. Our data indicate that actin dynamics is required for the increased life span of the sch9Δ, tor1∆, and ras2∆ mutants. Because RIM15, GIS1, and MSN2/4 are effectors of Tor1, Sch9, or Ras2, which are also shown to regulate life span, we speculate that some of them may be required for maintenance of the dynamic actin pattern in tor1∆, sch9Δ, and ras2∆ mutants. The fact that the actin cytoskeleton dynamics in sch9Δ depends on RIM15 and MSN2/4 but not GIS1, and in tor1∆ and ras2∆, depends on RIM15, suggests that these effectors also control actin dynamics in sch9Δ, tor1∆ and ras2∆ mutants. Our study suggested that dynamic actin pattern is required for longevity of sch9Δ, tor1∆ and ras2∆ mutants. These results will help us to further understand the role of actin in aging.

The mechanism that these mutants regulate actin dynamics, however, is still unclear. In budding yeast, Tor1 functions upstream of the Rho1-Pkc1 pathway, which was shown to regulates actin dynamics [18]. Ho and Bretscher demonstrated that deletion of RAS2 results in loss of Cdc42p and F-actin polarity [19], and it was suggested that Ras2 regulates actin cytoskeleton dynamics via the actin regulatory factor Srv2 [20]. Although there is no direct evidence shows that Sch9 regulates actin dynamics, recent study indicated that Sch9 is a key regulator of sphingolipid metabolism [21], which was shown to be able to regulate actin cytoskeleton through Ypk1 and Pkc1 [22]. These data suggest that Sch9 may indirectly controls actin dynamics. In addition, the effectors of Rim15, Gis1, and Msn2/4 may also mediate actin dynamic pattern during aging. Further investigation is required to answer these questions.

Experimental procedures

Yeast strains and media

Standard methods were used for yeast genetic manipulations [23]. All strains used in this study are listed in Table 1. The deletion mutants were created according to the procedure described previously [24] and confirmed by sequencing.

Table 1 Yeast strains used in this study
Full size table

To avoid possible effects due to auxotrophic deficiencies of the strains, the liquid synthetic complete dextrose (SCD) medium was supplemented with a 4-fold excess of tryptophan, leucine, uracil, and histidine (17) and 2% dextrose was used as a carbon source. This, we called “aging medium.” For YPD plates, 19 g L−1 bacto-yeast extract, 20 g L−1 bacto-peptone, and 20 g L−1 bacto-agar (Becton, Dickenson and Company) were dissolved in water and autoclaved and dextrose was added to a final concentration of 2%. YPE and YPG plates were made in the same way as YPD plates, except that 3% ethanol or 3% glycerol was used as a carbon source instead of dextrose.

Chronological life span assay

The chronological life span was measured according to the previous report [25]. Briefly, a small amount of yeast strain from the frozen (−80°C) sample was streaked onto an YPD plate and incubated at 30°C for 2–3 days. Five to six colonies were inoculated into 2 ml YPD liquid medium to make the saturated overnight culture (SONC). The fresh SONC was diluted in 25 ml of liquid medium to an initial density of ~1 × 106 cells mL−1 (A600 = 0.1). The flasks were maintained at 30°C at 220 rpm in the air bath shaker. The initial time point began at day 0, the next day was day 1, and so on. At day 3, the cells reached the stationary phase and stopped dividing, at which point the cells were deemed to be 100% viable. To determine the number of viable cells, 100-μl aliquots were removed from each culture and diluted serially to reach a 1:105 dilution in sterile distilled water. Two dilutions per culture were used routinely, and 100 μl of the dilution was plated onto YPD plates in duplicate. After incubation for 2–3 days, the number of colonies was counted, and the viability at this time point was calculated by comparing the numbers to day 0.

Microscopy

Phalloidin-TRITC (Sigma, P1951) stain was performed as previously reported [26]. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to growth medium to a final concentration of 3.7%. After washing with 1× PBS solution three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h. A Nikon Eclipse E600 fluorescence microscope equipped with a Plan Apo 100× 1.4 oil immersion objective was used for fluorescence microscopy. The images were captured with a SPOT RT 9.0 onochrome-6 camera using the MetaMorph (version 6.3.0) acquisition software. The whole cell reconstruction was gained by confocal microscopy (Leica TSC SP2/AOBS). The thickness of the Z-sections is 400 nm. Images were processed by Adobe Photoshop (version 7.0).

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 81173123) and Liaoning Natural Science Foundation (NO.2013225049). We are very grateful to Dr. Robert C. Dickson (University of Kentucky), who gave the author’s post-doc training in the aging-related academic field and his generous stains, plasmids and technical assistance.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Key Laboratory of Cell Biology, Ministry of Public Health, China Medical University, Shenyang, 110001, P. R. China

    Ying Liu, Nanqi Liu, Didi Wu & Qiang Bi

  2. Department of Pharmaceutics, Pharmacy school of China Medical University, Shenyang, 110001, P. R. China

    Shengnan Meng

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Authors’ contributions

YL designed and conducted all experiments, analyzed the data and drafted the manuscript. NL and DW helped with the conduction of the study. QB and SM helped with data analysis and the drafting of the manuscript. All authors read and approved the final manuscript.

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Liu, Y., Liu, N., Wu, D. et al. The longevity of tor1Δ, sch9Δ, and ras2Δ mutants depends on actin dynamics in Saccharomyces cerevisiae . Cell Biosci 5, 18 (2015). https://doi.org/10.1186/s13578-015-0008-z

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Cell & Bioscience

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