SLA1 is required for actin dynamics and chronological life span in DBY746, tor1, sch9Δ, and ras2Δ cells. (A)
tor1Δ sla1Δ (LY113), sch9Δ sla1Δ (LY114), and ras2Δ sla1Δ (LY115) showed less actin dynamics than tor1Δ (LY101), sch9Δ (LY102), and ras2Δ (LY103) single mutants. Cells were fixed for 1 h by adding 37% formaldehyde solution directly to growth medium to final concentration of 3.7%. After washing with 1× PBS solution for three times, cells were stained with phalloidin-TRITC in the dark at room temperature for 1 h and images were taken by fluorescent microscope. Scale bar, 5 μm. (B)
tor1Δsla1Δ (LY113), sch9Δsla1Δ (LY114), and ras2Δsla1Δ (LY115) double mutants have decreased life spans compared to single mutants. Sla1 is associated with actin patches and cables, which organizes the yeast actin cytoskeleton.