Figure 1

The actin structure is more dynamic in longevity mutants. (A) Wild type (DBY746), tor1Δ (LY101), sch9Δ (LY102), and ras2Δ (LY103) cells were cultured in aging medium. Triplicate cultures for each strain were used and experiments were repeated three times. Error bars represent standard errors. (B) The actin dynamic pattern was examined on day 1, 3, and 5 for yeast strains in (A). *,p < 0.01. (C) Actin is dynamic in DBY746, tor1, sch9Δ, and ras2Δ. The cells were treated with 50 μM latrunculin A, and after 5 and 10 min the actin pattern was examined by fluorescent microscopy. Scale bar, 5 μm. (D) The actin dynamic pattern was quantified for yeast strains in (C).