Skip to main content

NF-κB RelA is a cell-intrinsic metabolic checkpoint restricting glycolysis

Abstract

An intrinsic link between metabolism and function in immune cells, and in particular macrophages, has been well established recently. However, the molecular mechanisms controlling the metabolic switch in these sentinel cells for their integral roles in host defense, inflammation, homeostasis, and pathogenesis remain largely unknown. Here, we identify the master transcription factor NF-κB RelA as a vital cell-intrinsic checkpoint restricting aerobic glycolysis to favor mitochondrial oxidative phosphorylation (OXPHOS) and “M2” activation (alternative anti-inflammatory and pro-tumorigenic activation, in contrast to classical pro-inflammatory and anti-tumor M1 activation) of macrophages under oncogenic stress. RelA specific knockdown or genetic deletion in macrophages causes metabolism to shift away from OXPHOS toward glycolysis, resulting in drastically decreased oxygen consumption but significantly increased lactate and ATP production. The metabolic change in RelA deficient cells is associated with the decrease in the expressions of the OXPHOS gene SCO2 as well as the M2 marker and function genes arginase-1 and VEGF. These data suggest that RelA induces SCO2 expression to enhance OXPHOS and restrict glycolysis in macrophages for their pro-tumorigenic activation.

Dear editor,

Macrophages are functionally highly plastic and play central roles in host defense, inflammation, homeostasis, and pathogenesis including lung cancer, the number one cancer killer of both men and women [1,2,3]. Recent studies have revealed the vital role of metabolism in shaping the phenotype and function of macrophages [1, 4]. Upon activation by pathogens, for example, aerobic glycolysis is induced in macrophages, providing rapid energy and initiating quick pro-inflammatory response to eliminate the invading pathogens. Following pathogen clearance, the metabolism is then switched toward oxidative phosphorylation (OXPHOS), offering long term energy and promoting anti-inflammatory response and wound healing. The glycolytic, pro-inflammatory and oxidative, anti-inflammatory macrophages are often simply called as M1 and M2 macrophages, respectively. Of note, blocking oxidative metabolism in macrophages prevents their M2 polarization and concomitantly drives them into an M1-like state, whereas forcing oxidative metabolism in glycolytic M1 macrophages skews them into an M2-like phenotype [4]. Not surprisingly, the highly adaptive metabolic engine and functional plasticity of macrophages important for host defense and homeostasis are often abused under various pathogenic conditions and cancer in particular. Tumor cells create a microenvironment to preserve OXPHOS and impede glycolysis in associated macrophages (TAMs), repressing M1-like anti-tumor activation and potentiating a more M2-like status to promote tumor progression, immune escape, and therapeutic resistance [2, 3, 5, 6].

Despite of the significant progress, it remains largely unknown how metabolic modes are switched at the molecular level to orchestrate the different functional statuses of macrophages at either physiological or pathogenic conditions [1, 4, 5]. In this regard, using lung cancer as a model we have demonstrated the importance of RelA (also known as p65), the prototypic member of the nuclear factor-κB (NF-κB) family of transcription factors that have been linked to almost all cancer types and inflammation-associated diseases, in both tumor biology and immunology [6,7,8,9,10]. Particularly, cell-intrinsic RelA not only renders TAMs resistant to the cytotoxicity of CD8+ cytotoxic T lymphocytes (CTLs) but also arms them with a superior capacity to suppress CTLs, thereby promoting lung cancer [6]. However, whether and how RelA regulates the metabolism and M2-like polarization of TAMs to repress anti-tumor immunity and drive tumorigenesis are yet to be determined.

Interestingly, RelA knockdown alone seemed sufficient to induce robust glycolysis in human monocytes/macrophages even under normal culture conditions, as indicated by the much more rapid color change to yellow of the medium culturing the THP-1 cells stably expressing RelA specific short hairpin RNAs (shRNAs) (Fig. 1A). Although THP-1 cells resemble primary monocytes and macrophages in morphology and differentiation properties, they are malignant leukaemia cells with large genomic aberrations that do not occur in primary macrophages or TAMs. To validate the promising data and more importantly to study the role RelA in the metabolism and polarization of TAMs, we examined OXPHOS and glycolytic rate in mouse primary wild type (WT) or RelA knockout (KO) macrophages cultured with the tumor-conditioned medium (TCM) of murine Lewis lung carcinoma (LLC) cells. In line with the fact that OXPHOS is the main metabolic mode of TAMs [1, 5], high oxygen consumption was detected in WT macrophages cultured in TCM (Fig. 1B). The oxygen consumption could be repressed to a basal level by the OXPHOS inhibitor antimycin A (AMA). Notably, the oxygen consumption of RelA KO macrophages cultured in the same TCM was decreased to the basal level. But in stark contrast, lactate and ATP productions were significantly increased in the same RelA KO macrophages (Fig. 1C). The increase in lactate and ATP in RelA KO macrophages could be efficiently prevented by the glycolytic inhibitor 2-deoxy-D-glucose (2-DG). Moreover, we found that synthesis of cytochrome c oxidase 2 (SCO2), a mitochondrial enzyme critical for OXPHOS, was markedly reduced in RelA KO macrophages (Fig. 1D). The SCO2 repression was specific, because no change was detected in the expression of several other metabolism-related genes, such as glucose transporter 3 (Glu3), phosphoglucomutase 2 (PGM2), ribonucleotide reductase M2 B (RRM2B) and tumor protein p53 (TP53). These data suggest that RelA induces SCO2 expression to ensure OXPHOS as the primary metabolic engine in TAMs (Additional file 1).

Fig. 1
figure 1

Critical role of RelA in the SCO2 expression, oxidative phosphorylation utilization and glycolysis restriction of macrophages. A Acidic metabolism of the monocyte/macrophage THP-1 cells by RelA knockdown. B Reduced oxygen consumption in RelA KO macrophages cultured in TCM. C Increased lactate production and ATP generation in RelA KO macrophages cultured in TCM. D Decreased Sco2 expression in RelA KO macrophages cultured in TCM. In BD, data represent means ± SEM (n = 3). * P < 0.05; ** P < 0.01; Student’s t test

Following the exciting results, we examined if RelA deletion affects the M2-like phenotype of TAMs. To do so, we compared the expression levels of arginase-1 and vascular endothelial growth factor (VEGF) in RelA KO and WT macrophages cultured in TCM. Arginase-1 and VEGF are the hallmarks and functional genes of M2 macrophages [1,2,3,4,5,6]. Consistent with the predominant M2-like phenotype of TAMs [1,2,3,4,5,6], WT macrophages cultured in TCM expressed high levels of arginase-1 and VEGF (Fig. 2). However, the expression levels of both genes were significantly lower in RelA KO macrophages cultured in the same TCM. Thus, in association with its role in driving metabolism toward OXPHOS, RelA pushes TAMs to an M2-like state.

Fig. 2
figure 2

Crucial role of RelA in M2-like activation of macrophages. A qRCR showing decreased Vegf and Arg1 in RelA KO macrophages cultured in TCM. Data represent means ± SEM (n = 3). **P < 0.01; Student’s t test. B Immunofluorescence staining showing decreased arginase-1 (ARG1, green) in RelA KO macrophages cultured in TCM. Blue: DAPI nuclear counterstain

Macrophages are the most abundant immune cells within lung tumor microenvironment [1,2,3, 5, 6] . During tumor initiation stages, they show an overall glycolytic M1 phenotype to prevent tumorigenesis. But later they become a general oxidative M2 status to promote tumor progression. Compared to the well-defined mechanisms underlying the opposite roles of M1 and M2 macrophages, the molecules governing the important switch remains largely unknown. In fact, this is a central question that needs to be addressed in the cancer and immunology fields. The data presented here suggest that RelA is a checkpoint of macrophages maintaining high OXPHOS activity to restrict glycolysis for M2-like activation and tumor pathogenesis. They thus provide new mechanistic insights into how metabolism is delicately regulated to shape the phenotype and function of macrophages and how the metabolism and function of macrophages is deregulated for pathogenesis, and in particular, cancer promotion (Additional file 2).

Availability of data and materials

All data generated or analysed during this study are included in this published article and its supplementary information files.

References

  1. Kolliniati O, Ieronymaki E, Vergadi E, Tsatsanis C. Metabolic regulation of macrophage activation. J Innate Immun. 2022;14(1):51–68.

    Article  CAS  PubMed  Google Scholar 

  2. Li M, He L, Zhu J, Zhang P, Liang S. Targeting tumor-associated macrophages for cancer treatment. Cell Biosci. 2022;12(1):85.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Zhou J, Qu Z, Sun F, Han L, Li L, Yan S, Stabile LP, Chen LF, Siegfried JM, Xiao G. Myeloid STAT3 promotes lung tumorigenesis by transforming tumor immunosurveillance into tumor-promoting inflammation. Cancer Immunol Res. 2017;5(3):257–68.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Galván-Peña S, O’Neill LA. Metabolic reprograming in macrophage polarization. Front Immunol. 2014;5:420.

    PubMed  PubMed Central  Google Scholar 

  5. de Goede KE, Driessen AJM, Van den Bossche J. Metabolic cancer-macrophage crosstalk in the tumor microenvironment. Biology (Basel). 2020;9(11):380.

    PubMed  Google Scholar 

  6. Li L, Han L, Sun F, Zhou J, Ohaegbulam KC, Tang X, Zang X, Steinbrecher KA, Qu Z, Xiao G. NF-κB RelA renders tumor-associated macrophages resistant to and capable of directly suppressing CD8+ T cells for tumor promotion. Oncoimmunology. 2018;7(6): e1435250.

    Article  PubMed  PubMed Central  Google Scholar 

  7. Sun F, Li L, Yan P, Zhou J, Shapiro SD, Xiao G, Qu Z. Causative role of PDLIM2 epigenetic repression in lung cancer and therapeutic resistance. Nat Commun. 2019;10(1):5324.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Sun F, Yan P, Xiao Y, Zhang H, Shapiro SD, Xiao G, Qu Z. NanoPDLIM2 enhanced efficacy of PD-1 blockade and chemotherapy in mouse lung cancers. eLife. 2023(12):RP89638. bioRxiv [Preprint]. 2023 Jul 25:2023.07.23.550248

  9. Sun F, Xiao Y, Shapiro SD, Qu Z, Xiao G. Critical and distinct roles of cell type-specific NF-κB2 in lung cancer. JCI Insight. 2023;accepted.

  10. Xiao G, Rabson AB, Young W, Qing G, Qu Z. Alternative pathways of NF-κB activation: a double-edged sword in health and disease. Cytokine Growth Factor Rev. 2006;17(4):281–93.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank Gutian Xiao and Steven D. Shapiro for their advice and constructive feedback.

Funding

This study is supported in part by the NIH National Institute of General Medical Sciences (NIGMS) grant R01 GM144890, National Cancer Institute (NCI) grant R01 CA258614, American Cancer Society (ACS) Research Scholar grant RSG-19-166-01-TBG, American Lung Association (ALA) Lung Cancer Discovery Award 821321, and Tobacco Related-Disease Research Program (TRDRP) Research Award T33IR6461.

Author information

Authors and Affiliations

Authors

Contributions

ZQ conceived and designed the study, originally generated THP-1 stable cell lines and identified abnormal metabolism in RelA knockdown THP-1 cells, led and contributed to all aspects of the analysis, and wrote the manuscript. LL and LH performed and analyzed all the experimental assays.

Corresponding author

Correspondence to Zhaoxia Qu.

Ethics declarations

Ethics approval and consent to participate

We have complied with all relevant ethical regulations for animal testing and research. The animal experiments were performed in accordance with the US National Institutes of Health (NIH) Guidelines on the Use of Laboratory Animals. All animals were maintained under pathogen-free conditions and used according to protocols approved by the Institutional Animal Care and Use Committee (IACAUC) of the University of Pittsburgh and the University of Southern California.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1:

Methods.

Additional file 2: Table S2.

 Primers used for qPCR. 

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, L., Han, L. & Qu, Z. NF-κB RelA is a cell-intrinsic metabolic checkpoint restricting glycolysis. Cell Biosci 14, 11 (2024). https://doi.org/10.1186/s13578-024-01196-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13578-024-01196-7

Keywords