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Explore a novel function of human condensins in cellular senescence

Cell & Bioscience volume 10, Article number: 147 (2020) Cite this article

Abstract

There are two kinds of condensins in human cells, known as condensin I and condensin II. The canonical roles of condensins are participated in chromosome dynamics, including chromosome condensation and segregation during cell division. Recently, a novel function of human condensins has been found with increasing evidences that they play important roles in cellular senescence. This paper reviewed the research progress of human condensins involved in different types of cellular senescence, mainly oncogene-induced senescence (OIS) and replicative senescence (RS). The future perspectives of human condensins involved in cellular senescence are also discussed.

Introduction

Condensins were firstly identified for their fundamental roles in establishment and maintenance of mitotic chromosome condensation in cell-free system from Xenopus laevis eggs [1, 2]. Until now, most multicellular eukaryotes reported have two kinds of condensins, termed as condensin I and condensin II [3,4,5,6]. The two kinds of condensins are also exist in human cells [7,8,9,10]. Both human condensins are pentameric complexes composed of shared core SMC2/SMC4 (structural maintenance of chromosomes, SMC) heterodimer(also known as hCAP-E/hCAP-C)and three accessory non-SMC subunits, including a kleisin subunit and two HEAT-repeat proteins. They are hCAP-H(NCAPH), hCAP-D2(NCAP-D2) and hCAP-G(NCAPG) for condensin I and hCAP-H2(NCAP-H2), hCAP-D3(NCAPD3) and hCAP-G2(NCAP-G2) for condensin II [10,11,12].

The canonical roles of human condensins are participated in chromosome dynamics, including chromosome condensation and segregation during mitosis [7,8,9,10,11,12]. Although both human condensins have similar components and alphabetic structure, they show different nuclear distribution, localization on chromosomes and play distinct roles in chromosome dynamics during mitosis [9,10,11,12,13].

In detail, during interphase condensin I is present in the cytoplasm, whereas condensin II is enriched within the nucleus [10, 14, 15]. During mitosis, initially condensin II participates in chromosome condensation within the nucleus in early prophase, whereas condensin I can interact with chromosomes only after the nuclear envelope breaks down [11]. Human condensin I shows a two-step dynamic binding. Once nuclear envelope breakdown, human condensin I rapidly associated with mitotic chromosomes then remained constant from prometaphase to late metaphase and chromatin bound human condensin I increased again just from anaphase onset until late anaphase when it dissociated from chromosomes[11, 12]. Similarly, human condensin I complexes dynamically bind to chromosomes in two steps during prometaphase and early anaphase whereas human condensin II complexes are stably bound to chromosomes throughout mitosis. Localization of human condensin II is centrally confined, but condensin I reaches ∼50% of the chromatid diameter from its center [16]. It is indicated that human condensin II but not condensin I is more indispensible for the salt-dependent, reversible reorganization of condensin II-based axes in chromosome shaping [17]. Moreover, human condensins show a discontinuous pattern along mitotic chromosomes and play a major role in controlling the elastic stiffness of metaphase chromosomes. Depletion of condensin II impacts chromosome mechanics more than depletion of condensin I and stiffness of the metaphase chromosome is more dependent on condensin II than on condensin I [18].This idea is somewhat inconsistent with a former study. It has been demonstrated that human condensin I but not condensin II can associate with KIF4A to confer rigidity to centromeres [19]. During anaphase, when human condensins are depleted, chromosomes are formed with improperly structured kinetochores and chromosome bridges appear in the cell [20]. Likewise, when human condensins are knocked down or dysfunction in human cells, chromatin bridges between daughter cells in anaphase and multiple nuclei in single cells are observed[21, 22]. During telophase, human condensins are involved in the mitotic chromosome conformation transformation into the interphase state as well. Recently, it is identified telophase as a critical transition between condensin- and cohesin-driven chromosome folding [23]. Consistently, human condensin II can initiate sister chromatid resolution during S phase [24]. Altogether, the differences in the timing of binding to chromosome and mutant phenotypes of dysfunction strongly indicated that human condensin I and II have fundamentally distinct functions during mitosis. Different nuclear distribution, localization on chromosomesof human condensin I and human condenisn II during cell cycle are shown as Fig. 1.

Fig. 1
figure 1

Different nuclear distribution, localization on chromosomes of human condensin I and condensin II during cell cycle (one chromosome is illustrated as an example)

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In addition to their mitotic functions, human condensins also play important roles in the prestressed condensed state of the nucleus, homologous recombination repair and gene expression during interphase [20, 24,25,26,27,28,29,30,31,32,33,34,35]. Beyond the multiple roles of human condensins mentioned above, increasing evidences show a novel function of human condensins in cellular senescence [36, 37].

Cellular senescence plays important protective roles in development, tissue homeostasis, wound healing, multiple age-related diseases and cancer [38, 39]. Cellular senescence is a stable state of irreversible cell cycle arrest caused by various forms of cellular stresses. Senescent cells lose DNA replication ability and still maintain cellular metabolic activity [40, 41]. Especially, cellular senescence can also be induced by pathophysiological stimuli, such as ROS (reactive oxygen species), oncogene activation, cytotoxic drugs and aging [38]. Nowadays four kinds of cellular senescence are recognized, i.e., oncogene-induced senescence (OIS), replicative senescence (RS), stress-induced premature senescence (SIPS) and therapy-induced senescence (TIS) [37, 38, 41, 42]. OIS is induced by oncogene expression and RS is induced by telomere shortening [37, 38, 41, 43,44,45]. SIPS is induced by various external signals, such as UV, hyperoxia, hydrogen peroxide, etc. [41, 45]. TIS is caused by traditional cancer therapy and it can be an effective way to treat cancer while lessening side effects [42, 46,47,48].

Although human condensins play classical roles in chromosome dynamics during mitosis, their nonmitotic functions have been payed more and more attention than before. As a novel function of human condensins in cellular senescence during interphase, much more problems remain to be further explored. This paper reviewed the research progress of human condensins involved in different types of cellular senescence, mainly oncogene-induced senescence (OIS) and replicative senescence (RS). The future perspectives of human condensins in cellular senescence are also discussed.

Human condensins involved in cellular senescence

Firstly, human condensins are involved in OIS. Initially, human condensin II is found to play a novel role in OIS. Overexpression of human condensin II, but not human condensin I, induces cellular senescence and senescence-associated heterochromatic foci (SAHF) formation and depletion of human condensin II inhibits the establishment of OIS [36]. In detail, the N-terminus truncated variant, hCAP-H2ΔN (lacking the first 50 amino acids) is mostly localized at the nuclear matrix and accumulates in quiescent and senescent cells. The ΔN variant exists as an insoluble nuclear structure while the full-length hCAP-H2 associates with mitotic chromosome. Overexpression of the full-length hCAP-H2 and ΔN variant can significantly induce senescence. Expression of hCAP-H2ΔN was increased during OIS. Moreover, hCAP-H2 knockdown (KD) also inhibited Ras-induced senescence. It is suggested that human condensin II drives senescence via nuclear/genomic reorganization [36]. Recently, the roles of human condensin II are reported to participate in cellular senescence through compartmental reorganizations coupled to gene regulation [37]. Human condensins strengthen and expand euchromatic A compartments and promote/maintain BA transitions upon senescence. Concretely, localization of hCAP-H2 is firstly studied and the results show they localize at active senescence genes, highly transcribed housekeeping genes, and potential enhancers. Next, by compared the general organization of the human genome into A and B compartments in OIS and growing cells, it is found that A compartments in OIS cells were significantly enlarged (~ 50%) compared to counterparts in growing cells. The increased BA transitions result in the significant enlargement of A compartments in OIS cells compared to growing cells. In efforts to find a functional link between senescence-dependent compartmental reorganizations and condensin, the following results show that human condensin II binding and dissociation are involved in BA and AB transitions and this mechanism is conserved between OIS and RS. In addition to promoting and maintaining BA transitions, human condensin II also play important roles to maintain euchromatic A compartments and facilitate genomic contacts in A compartments, that is, to reinforce A compartments. Further research suggests that human condensin II plays a direct role in the upregulation of senescence genes because that many genes upregulated upon OIS and downregulated by hCAP-H2 KD (knock down) are hCAP-H2 binding genes. Collectively, human condensins are not only involved in euchromatic A compartments and BA transitions, but also involved in the upregulation of senescence genes upon senescence. Therefore, the roles of human condensin II in cellular senescence may be through compartmental reorganizations coupled to gene regulation [37].

Secondly, human condensins are involved in RS. SMC2 and SMC4, core subunits of human condensins, are demonstrated to be down-regulated in the serially passaged fibroblast cells by proteomic study and they are supposed to play an important role in RS [41]. Consistently, several subunits of human condensin I and II (mainly non-SMC subunits, i.e., hCAP-D2, hCAP-D3, hCAP-G, hCAP-G2, hCAP-H and SMC4) are downregulated in KDM3A- or KDM4C-knockdown human umbilical cord-derived stromal cells (hUCMSCs) or upregulated in KDM3A or KDM4C-overexpressing hUCMSCs (49). Especially, hCAPD-2 and hCAPG-2 are positively regulated by KDM3A and KDM4C with their H3K9 demethylase activity and human condensins regulated by KDM3A or KDM4 might be critical for stability of the heterochromatin structure during senescence. Recently,as mentioned above, human condensin II is involved in BA and AB transitions not only during OIS but also during RS [37].

Hitherto, there are few literatures published from the point that human condensins are involved in stress-induced premature senescence (SIPS) and therapy-induced senescence (TIS) [36, 37, 49]. Abnormal expression of subunits of human condensins and changes of structure of chromosomes in four types of cellular senescence are shown in Table 1.

Table 1 Abnormal expression of subunits of human condensins and changes of structure of chromosomes in four types of cellular senescence
Full size table

Conclusions

Apart from the multiple canonical functions of chromosome dynamics played by human condensins during mitosis and interphase, increasing evidences show a novel function of human condensins in cellular senescence. Human condensins play important roles in the main two types of cellular senescence, i.e. oncogene-induced senescence (OIS) and replicative senescence (RS).

Future perspectives of human condensins in cellular senescence

To explore a novel function of human condensins in cellular senescence, three future perspectives are presented as follows.

Firstly, how human condensins involved in interphase nuclear reorganization during cellular senescence needs further study. Both human condensin I and II are required for maintenance of the interphase nuclear architecture. Human condensins regulated by KDM3A or KDM4 might be critical for stability of the heterochromatin structure during senescence [49]. Depletion of human condensin II leads to dramatic disruption of nuclear architecture and nuclear size [50]. Interaction between human condensin II and SAHF provide an additional platform for studies on condensins participating dynamic interphase chromatin reorganization [51]. Recently, human condensin II subunit hCAP-H2 is demonstrated to associate with shelterin protein TRF1 and be required for telomere stability [52]. Furthermore, there is small fraction of human condensin I retained and be active in both gene regulation and chromosome condensation in interphase nuclei [53,54,55]. Although human condensin II is reported to be involved in SAHF formation and BA and AB transitions, whether human condensin I involved these process is still unclear [36, 37]. It is also intriguing to explore what roles of human condensin I play in cellular senescence during interphase.

Secondly, the different functions and possible interplays of human condensins in cellular senescence, quiescence and carcinogenesis needs further study. Although cell cycle differences exist between cellular senescence and quiescence, either cellular senescence or quiescence misregulation is implicated in cancer progression [39, 42, 56, 57]. It is surprising that condensins are involved in all the three processes [36, 37, 41, 49, 58,59,60,61,62]. In a mutant mouse carrying a constitutive missense mutation in the condensin II kleisin-β subunit Caph2, the mutation specifically causes chromatin decondensation and condensin II is demonstrated to be required for peripheral T-cell development and maintenance of the quiescent state [58,59,60]. With further analysis, the mutant mice show condensin II-dependent thymic lymphomas formation through tissue-specific genome instability [61]. Recently, in the quiescent state of Saccharomyces cerevisiae, condensin is required for widespread transcriptional silencing and dramatic chromatin condensation through binding throughout the budding yeast genome and induces the formation of large chromatin loop domains [62]. Hitherto, apart from cellular senescence and tumorgenensis, whether human condensins play a role in quiescence is unclear. In addition, how human condensins involved the three processes need be extensively explored [41, 49, 52, 62]. It is promising to explore possible interplays of cellular senescence and tumor formation and anticancer therapy from the viewpoints of human condensins [63,64,65].

Finally, whether some crosstalks exist in condensins, cohesins and SMC5/SMC6 complexes is yet unclear during human cellular senescence. SMC complexes have ancient origins and share structural similarities. Condensin, cohesin and SMC5/SMC6 complex are three types of evolutionarily conserved SMC complexes within eukaryotic cells and the three complexes are all reported to be involved in cellular senescence in different organisms. Concretely, loss of the recruitment cohesin and condensin I complexes to pericentromeric regions causes to block efficient repair of the regions and leads to formation of persistent DNA damage foci in senescent human adult stem cells [66]. Depletion of human SMC5/6 subunits by RNAi inhibits telomere homologous recombination and causing telomere shortening and cellular senescence in human ALT (ALT, alternative lengthening of telomeres) cells [67, 68]. Similarly, SMC5/SMC6 complex as a target of Mms21-dependent sumoylation is also involved in cellular senescence in Saccharomyces cerevisiae [69]. Of note, interaction of condensin and cohesin is reported as a chromosome folding intermediate during telophase as a critical transition between condensin- and cohesin-driven chromosome folding [23]. Distinct roles of cohesin and condensin are required in the establishment of 3D nuclear organization in Drosophila [70]. Common kleisin-hinge interaction and different modes of regulation are also proposed in condensin and cohesin in fission yeast [71]. Likewise, functional interplay between cohesin and Smc5/6 complexes is reviewed for significant overlap of their location, function and crosstalk between these two complexes [72]. Similarly, there are also papers about interactions with condensin and Smc5/6 complexes. Depletion of Smc5 and Smc6 results in abnormal distribution of condensins and chromosome segregation errors in human cells [73]. Furthermore, mutation of SMC5 also leads to abnormal distribution of condensin along chromosomes with decreased condensin accumulation at pericentromeric regions and enrichment of condensin on chromosome arms in mouse embryonic stem cells (mESCs) [74]. There are complex chromatin reorganizations in senescent cells [75,76,77]. Recently, different models of SMC complex function have been presented [78,79,80]. Based on all mentioned above, we propose that senescence-associated chromatin reorganization may need cooperative function of the three SMC complexes, but the molecular mechanism is yet unknown.

Taken together, human condensins play important roles in cellular senescence. It is worthy to be explored for the new multifunctions of human condensins in cellular senescence.

Availability of data and materials

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References

  1. Hirano T, Mitchison TJ. A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro. Cell. 1994;79:449–58. https://doi.org/10.1016/0092-8674(94)90254-2.

    Article  CAS  PubMed  Google Scholar 

  2. Hirano T, Kobayashi R, Hirano M. Condensins chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila barren protein. Cell. 1997;89:511–21. https://doi.org/10.1016/s0092-8674(00)80233-0.

    Article  CAS  PubMed  Google Scholar 

  3. Lišková L, Šušor A, Pivoňková K, Sasková A, Karabínová P, Kubelka M. Detection of condensin I and II in maturing pig oocytes. Rep Fertil Dev. 2010;22:644–52. https://doi.org/10.1071/RD09068.

    Article  CAS  Google Scholar 

  4. Lee J, Ogushi S, Saitou M, Hirano T. Condensins I and II are essential for construction of bivalent chromosomes in mouse oocytes. Mol Biol Cell. 2011;22:3465–77. https://doi.org/10.1091/mbc.E11-05-0423.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Fujiwara T, Tanaka K, Kuroiwa T, Hirano T. Spatiotemporal dynamics of condensins I and II: evolutionary insights from the primitive red alga Cyanidioschyzon merolae. Mol Biol Cell. 2013;24:2515–27. https://doi.org/10.1091/mbc.E13-04-0208.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Sakamoto T, Sugiyama T, Yamashita T, Matsunaga S. Plant condensin II is required for the correct spatial relationship between centromeres and rDNA arrays. Nucleus. 2019;10:116–25. https://doi.org/10.1080/19491034.2019.1616507.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Schmiesing JA, Gregson HC, Zhou S, Yokomori K. A human condensin complex containing hCAP-C-hCAP-E and CNAP1, a homolog of Xenopus XCAP-D2, colocalizes with phosphorylated histone H3 during the early stage of mitotic chromosome condensation. Mol Cell Biol. 2000;20:6996–7006. https://doi.org/10.1128/mcb.20.18.6996-7006.2000.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Yeong FM, Hombauer H, Wendt KS, Hirota T, Mudrak I, Mechtler K, Loregger T, Marchler-Bauer A, Tanaka K, Peters JM, Ogris E. Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A. Curr Biol. 2003;13:2058–64. https://doi.org/10.1016/j.cub.2003.10.032.

    Article  CAS  PubMed  Google Scholar 

  9. Kimura K, Cuvier O, Hirano T. Chromosome condensation by a human condensin complex in Xenopus egg extracts. J Biol Chem. 2001;276:5417–20. https://doi.org/10.1074/jbc.C000873200.

    Article  CAS  PubMed  Google Scholar 

  10. Ono T, Losada A, Hirano M, Myers MP, Neuwald AF, Hirano T. Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells. Cell. 2003;115:109–21. https://doi.org/10.1016/s0092-8674(03)00724-4.

    Article  CAS  PubMed  Google Scholar 

  11. Ono T, Fang Y, Spector DL, HiranoT. Spatial and temporal regulation of condensins I and II in mitotic chromosome assembly in human cells. Mol Biol Cell. 2004;15:3296–308. https://doi.org/10.1091/mbc.e04-03-0242.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Hirota T, Gerlich D, Koch B, Ellenberg J, Peters JM. Distinct functions of condensin I and II in mitotic chromosome assembly. J Cell Sci. 2004;117:6435–45. https://doi.org/10.1242/jcs.01604.

    Article  CAS  PubMed  Google Scholar 

  13. Onn I, Aono N, Hirano M, Hirano T. Reconstitution and subunit geometry of human condensin complexes. EMBO J. 2007;26:1024–34. https://doi.org/10.1038/sj.emboj.7601562.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Gerlich D, Hirota T, Koch B, Peters JM, Ellenberg J. Condensin I stabilizes chromosomes mechanically through a dynamic interaction in live cells. Curr Biol. 2006;16:333–44. https://doi.org/10.1016/j.cub.2005.12.040.

    Article  CAS  PubMed  Google Scholar 

  15. Hirano T. Condensin-based chromosome organization from bacteria to vertebrates. Cell. 2016;164:847–57. https://doi.org/10.1016/j.cell.2016.01.033.

    Article  CAS  PubMed  Google Scholar 

  16. Walther N, Hossain MJ, Politi AZ, Koch B, Kueblbeck M, Ødegård-Fougner Ø, Lampe M, Ellenberg J. A quantitative map of human Condensins provides new insights into mitotic chromosome architecture. J Cell Biol. 2018;217:2309–28. https://doi.org/10.1083/jcb.201801048.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Ono T, Sakamoto C, Nakao M, Saitoh N, Hirano T. Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ. Mol Biol Cell. 2017;28:2875–86. https://doi.org/10.1091/mbc.E17-04-0252.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Sun M, Biggs R, Hornick J, Marko JF. Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold. Chromosome Res. 2018;26:277–95. https://doi.org/10.1007/s10577-018-9584-1.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Takahashi M, Wakai T, Hirota T. Condensin I-mediated mitotic chromosome assembly requires association with chromokinesin KIF4A. Genes Dev. 2016;30:1931–6. https://doi.org/10.1101/gad.282855.116.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Samoshkin A, Arnaoutov A, Jansen LE, Ouspenski I, Dye L, Karpova T, McNally J, Dasso M, Cleveland DW, Strunnikov A. Human condensin function is essential for centromeric chromatin assembly and proper sister kinetochore orientation. PLoS ONE. 2009;4(8):e6831. https://doi.org/10.1371/journal.pone.0006831.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Zhai L, Wang HZ, Tang W, Liu WG, Hao S, Zeng XL. Disturbance in function and expression of condensin affects chromosome compaction in HeLa cells. Cell Biol Int. 2011;35:735–40. https://doi.org/10.1042/CBI20100646.

    Article  PubMed  Google Scholar 

  22. Samoshkin A, Dulev S, Loukinov D, Rosenfeld JA, Strunnikov AV. Condensin dysfunction in human cells induces nonrandom chromosomal breaks in anaphase, with distinct patterns for both unique and repeated genomic regions. Chromosoma. 2012;121:191–9. https://doi.org/10.1007/s00412-011-0353-6.

    Article  CAS  PubMed  Google Scholar 

  23. Abramo K, Valton AL, Venev SV, Ozadam H, Fox AN, Dekker J. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol. 2019;21:1393–402. https://doi.org/10.1038/s41556-019-0406-2.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Ono T, Yamashita D, Hirano T. Condensin II initiates sister chromatid resolution during S phase. J Cell Biol. 2013;200:429–41. https://doi.org/10.1083/jcb.201208008.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Takemoto A, Kimura K, Yanagisawa J, Yokoyama S, Hanaoka F. Negative regulation of condensin I by CK2-mediated phosphorylation. EMBO J. 2006;25:5339–48. https://doi.org/10.1038/sj.emboj.7601394.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. Barnhart-Dailey MC, Trivedi P, Stukenberg PT, Foltz DR. HJURP interaction with the condensin II complex during G1 promotes CENP-A deposition. Mol Biol Cell. 2017;28:54–64. https://doi.org/10.1091/mbc.E15-12-0843.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Trimborn M, Bell SM, Felix C, Rashid Y, Jafri H, Griffiths PD, Neumann LM, Krebs A, Reis A, Sperling K, Neitzel H, Jackson AP. Mutations in microcephalin cause aberrant regulation of chromosome condensation. Am J Hum Genet. 2004;75:261–6. https://doi.org/10.1086/422855.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Trimborn M, Schindler D, Neitzel H, Hirano T. Misregulated chromosome condensation in MCPH1 primary microcephaly is mediated by condensin II. Cell Cycle. 2006;5:322–6. https://doi.org/10.4161/cc.5.3.2412.

    Article  CAS  PubMed  Google Scholar 

  29. Wood JL, Liang Y, Li K, Chen J. Microcephalin/MCPH1 associates with the condensin II complex to function in homologous recombination repair. J Biol Chem. 2008;283:29586–92. https://doi.org/10.1074/jbc.M804080200.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Yamashita D, Shintomi K, Ono T, Gavvovidis I, Schindler D, Neitzel H, Trimborn M, Hirano T. MCPH1 regulates chromosome condensation and shaping as a composite modulator of condensin II. J Cell Biol. 2011;194:841–54. https://doi.org/10.1083/jcb.201106141.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Heale JT, Ball AR Jr, Schmiesing JA, Kim JS, Kong X, Zhou S, Hudson DF, Earnshaw WC, Yokomori K. Condensin I interacts with the PARP-1-XRCC1 complex and functions in DNA single-strand break repair. Mol Cell. 2006;21:837–48. https://doi.org/10.1016/j.molcel.2006.01.036.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Kong X, Stephens J, Ball AR Jr, Heale JT, Newkirk DA, Berns MW, Yokomori K. Condensin I recruitment to base damage-enriched DNA lesions is modulated by PARP. 1PLoS One. 2011;6(8):e23548. https://doi.org/10.1371/journal.pone.0023548.

    Article  CAS  Google Scholar 

  33. Zhang T, Si-Hoe SL, Hudson DF, Surana U. Condensin recruitment to chromatin is inhibited by Chk2 kinase in response to DNA damage. Cell Cycle. 2016;15:3454–70. https://doi.org/10.1080/15384101.2016.1249075.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Ward JR, Vasu K, Deutschman E, Halawani D, Larson PA, Zhang D, Willard B, Fox PL, Moran JV, Longworth MS. Condensin II and GAIT complexes cooperate to restrict LINE-1 retrotransposition in epithelial cells. PLoS Genet. 2017;13:e1007051. https://doi.org/10.1371/journal.pgen.1007051.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Huang K, Jia J, Wu C, Yao M, Li M, Jin J, Jiang C, Cai Y, Pei D, Pan G, Yao H. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem. 2013;288:26067–77. https://doi.org/10.1074/jbc.M113.486175.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Yokoyama Y, Zhu H, Zhang R, Noma K. A novel role for the condensin II complex in cellular senescence. Cell Cycle. 2015;14:2160–70. https://doi.org/10.1080/15384101.2015.1049778.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  37. Iwasaki O, Tanizawa H, Kim KD, Kossenkov A, Nacarelli T, Tashiro S, Majumdar S, Showe LC, Zhang R, Noma KI. Involvement of condensin in cellular senescence through gene regulation and compartmental reorganization. Nat Commun. 2019;10:5688. https://doi.org/10.1038/s41467-019-13604-5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Parry AJ, Narita M. Old cells, new tricks: chromatin structure in senescence. Mamm Genome. 2016;27:320–31. https://doi.org/10.1007/s00335-016-9628-9.

    Article  PubMed  PubMed Central  Google Scholar 

  39. Kim YH, Park TJ. (2019) Cellular senescence in cancer. BMB Rep 52:42–6. https://doi.org/10.5483/BMBRep.2019.52.1.295.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  40. Salama R, Sadaie M, Hoare M, Narita M. (2014) Cellular senescence and its effector programs. Genes Dev 28:99–114. https://doi.org/10.1101/gad.235184.113.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  41. Meng Q, Gao J, Zhu H, He H, Lu Z, Hong M, Zhou H. (2018) The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence. Biochem Biophys Res Commun 505:1112–20. https://doi.org/10.1016/j.bbrc.2018.10.065.

    Article  CAS  PubMed  Google Scholar 

  42. Lee S, Lee JS. Cellular senescence: a promising strategy for cancer therapy. BMB Rep. 2019;52:35–41. https://doi.org/10.5483/BMBRep.2019.52.1.294.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Serrano M, Lin AW, McCurrach ME, Beach D, Lowe SW. Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a. Cell. 1997;88:593–602. https://doi.org/10.1016/s0092-8674(00)81902-9.

    Article  CAS  PubMed  Google Scholar 

  44. Gorgoulis VG, Halazonetis TD. Oncogene-induced senescence: the bright and dark side of the response. Curr Opin Cell Biol. 2010;22:816–27. https://doi.org/10.1016/j.ceb.2010.07.013.

    Article  CAS  PubMed  Google Scholar 

  45. de Magalhães JP, Passos JF. Stress, cell senescence and organismal ageing. Mech Ageing Dev. 2018;170:2–9. https://doi.org/10.1016/j.mad.2017.07.001.

    Article  CAS  PubMed  Google Scholar 

  46. Chang BD, Broude EV, Dokmanovic M, Zhu H, Ruth A, Xuan Y, Kandel ES, Lausch E, Christov K, Roninson IB. A senescence-like phenotype distinguishes tumor cells that undergo terminal proliferation arrest after exposure to anticancer agents. Cancer Res. 1999;59:3761–7.

    CAS  PubMed  Google Scholar 

  47. Chang BD, Xuan Y, Broude EV, Zhu H, Schott B, Fang J, Roninson IB. Role of p53 and p21waf1/cip1 in senescence-like terminal proliferation arrest induced in human tumor cells by chemotherapeutic drugs. Oncogene. 1999;18:4808–18. https://doi.org/10.1038/sj.onc.1203078.

    Article  CAS  PubMed  Google Scholar 

  48. Bojko A, Czarnecka-Herok J, Charzynska A, Dabrowski M, Sikora E. Diversity of the senescence phenotype of cancer cells treated with chemotherapeutic agents. Cells. 2019;8:1501. https://doi.org/10.3390/cells8121501.

    Article  CAS  PubMed Central  Google Scholar 

  49. Huang B, Wang B, Yuk-Wai Lee W, Pong UK, Leung KT, Li X, Liu Z, Chen R, Lin JC, Tsang LL, Liu B, Ruan YC, Chan HC, Li G, Jiang X. KDM3A and KDM4C regulate mesenchymal stromal cell senescence and bone aging via condensin-mediated heterochromatin reorganization. iScience. 2019;21:375–90. https://doi.org/10.1016/j.isci.2019.10.041.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. George CM, Bozler J, Nguyen HQ, Bosco G. (2014) Condensins are required for maintenance of nuclear architecture. Cells 3:865–82. https://doi.org/10.3390/cells3030865.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  51. Ito Y, Narita M. The expanding territories of condensin II. Cell Cycle. 2015;14:2723–4. https://doi.org/10.1080/15384101.2015.1063356.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  52. Wallace HA, Rana V, Nguyen HQ, Bosco G. Condensin II subunit NCAPH2 associates with shelterin protein TRF1 and is required for telomere stability. J Cell Physiol. 2019;234:20755–68. https://doi.org/10.1002/jcp.28681.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  53. Cabello OA, Eliseeva E, He WG, Youssoufian H, Plon SE, Brinkley BR, Belmont JW. Cell cycle-dependent expression and nucleolar localization of hCAP-H. Mol Biol Cell. 2001;12:3527–37. https://doi.org/10.1091/mbc.12.11.3527.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. Zhang T, Paulson JR, Bakhrebah M, Kim JH, Nowell C, Kalitsis P, Hudson DF. Condensin I and II behaviour in interphase nuclei and cells undergoing premature chromosome condensation. Chromosome Res. 2016;24:243–69. https://doi.org/10.1007/s10577-016-9519-7.

    Article  CAS  PubMed  Google Scholar 

  55. Xing X, Mroß C, Hao L, Munck M, Herzog A, Mohr C, Unnikannan CP, Kelkar P, Noegel AA, Eichinger L, Neumann S. Nesprin-2 interacts with condensin component SMC2. Int J Cell Biol. 2017;2017:8607532. https://doi.org/10.1155/2017/8607532.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. Swygert SG, Kim S, Wu X, Fu T, Hsieh TH, Rando OJ, Eisenman RN, Shendure J, McKnight JN, Tsukiyama T. Condensin-dependent chromatin compaction represses transcription globally during quiescence. Mol Cell. 2019;73:533–46.e4. https://doi.org/10.1016/j.molcel.2018.11.020.

    Article  CAS  PubMed  Google Scholar 

  57. Matson JP, House AM, Grant GD, Wu H, Perez J, Cook JG. Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence. J Cell Biol. 2019;218:2169–84. https://doi.org/10.1083/jcb.201902143.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  58. Gosling KM, Makaroff LE, Theodoratos A, Kim YH, Whittle B, Rui L, Wu H, Hong NA, Kennedy GC, Fritz JA, Yates AL, Goodnow CC, Fahrer AM. A mutation in a chromosome condensin II subunit, kleisin beta, specifically disrupts T cell development. Proc Natl Acad Sci U S A. 2007;104:12445–50. https://doi.org/10.1073/pnas.0704870104.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  59. Gosling KM, Goodnow CC, Verma NK, Fahrer AM. Defective T-cell function leading to reduced antibody production in a kleisin-beta mutant mouse. Immunology. 2008;125:208–17. https://doi.org/10.1111/j.1365-2567.2008.02831.x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Rawlings JS, Gatzka M, Thomas PG, Ihle JN. Chromatin condensation via the condensin II complex is required for peripheral T-cell quiescence. EMBO J. 2011;30:263–76. https://doi.org/10.1038/emboj.2010.314.

    Article  CAS  PubMed  Google Scholar 

  61. Woodward J, Taylor GC, Soares DC, Boyle S, Sie D, Read D, Chathoth K, Vukovic M, Tarrats N, Jamieson D, Campbell KJ, Blyth K, Acosta JC, et al. Condensin II mutation causes T-cell lymphoma through tissue-specific genome instability. Genes Dev. 2016;30:2173–86. https://doi.org/10.1101/gad.284562.116.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  62. Swygert SG, Tsukiyama T. Unraveling quiescence-specific repressive chromatin domains. Curr Genet. 2019;65:1145–51. https://doi.org/10.1007/s00294-019-00985-9.

    Article  CAS  PubMed  Google Scholar 

  63. Wang HZ, Yang SH, Li GY, Cao X. Subunits of human condensins are potential therapeutic targets for cancers. Cell Div. 2018;13:2. https://doi.org/10.1186/s13008-018-0035-3.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  64. Hoare MW, Narita M. The power behind the throne: senescence and the hallmarks of cancer. Annu Rev Cancer Biol. 2018;2:175–94. https://doi.org/10.1146/annurev-cancerbio-030617-050352.

    Article  Google Scholar 

  65. Chan ASL, Narita M. Short-term gain, long-term pain: the senescence life cycle and cancer. Genes Dev. 2019;33:127–43. https://doi.org/10.1101/gad.320937.118.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  66. Wang J, Geesman GJ, Hostikka SL, Atallah M, Blackwell B, Lee E, Cook PJ, Pasaniuc B, Shariat G, Halperin E, Dobke M, Rosenfeld MG, Jordan IK, et al. Inhibition of activated pericentromeric SINE/Alu repeat transcription in senescent human adult stem cells reinstates self-renewal. Cell Cycle. 2011;10:3016–30. https://doi.org/10.4161/cc.10.17.17543.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  67. Potts PR, Yu H. The SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins. Nat Struct Mol Biol. 2007;14:581–90. https://doi.org/10.1038/nsmb1259.

    Article  CAS  PubMed  Google Scholar 

  68. Min J, Wright WE, Shay JW. Alternative lengthening of telomeres mediated by mitotic DNA synthesis engages break-induced replication processes. Mol Cell Biol. 2017;37:e00226-17. https://doi.org/10.1128/MCB.00226-17.

    Article  PubMed  PubMed Central  Google Scholar 

  69. Chavez A, George V, Agrawal V, Johnson FB. Sumoylation and the structural maintenance of chromosomes (Smc) 5/6 complex slow senescence through recombination intermediate resolution. J Biol Chem. 2010;285:11922–30. https://doi.org/10.1074/jbc.M109.041277.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  70. Rowley MJ, Lyu X, Rana V, Ando-Kuri M, Karns R, Bosco G, Corces VG. Condensin II counteracts cohesin and RNA polymerase II in the establishment of 3D chromatin organization. Cell Rep. 2019;26(11):2890–903.e3. https://doi.org/10.1016/j.celrep.2019.01.116.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  71. Xu X, Yanagida M. Suppressor screening reveals common kleisin-hinge interaction in condensin and cohesin, but different modes of regulation. Proc Natl Acad Sci USA. 2019;116:10889–98. https://doi.org/10.1073/pnas.1902699116.

    Article  CAS  PubMed  Google Scholar 

  72. Tapia-Alveal C, Lin SJ, O’Connell MJ. Functional interplay between cohesin and Smc5/6 complexes. Chromosoma. 2014;123:437–45. https://doi.org/10.1007/s00412-014-0474-9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  73. Gallego-Paez LM. Tanaka H, Bando M. Takahashi M, Nozaki N. Nakato R, Shirahige K. Hirota T (2014) Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302 – 17. https://doi.org/10.1091/mbc.E13-01-0020.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  74. Pryzhkova MV, Jordan PW. Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. J Cell Sci. 2016;129:1619–34. https://doi.org/10.1242/jcs.179036.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  75. Swanson EC, Rapkin LM, Bazett-Jones DP, Lawrence JB. Unfolding the story of chromatin organization in senescent cells. Nucleus. 2015;6(4):254–60. https://doi.org/10.1080/19491034.2015.1057670.

    Article  PubMed  PubMed Central  Google Scholar 

  76. Graziano S, Gonzalo S. Mechanisms of oncogene-induced genomic instability. Biophys Chem. 2017;225:49–57. https://doi.org/10.1016/j.bpc.2016.11.008.

    Article  CAS  PubMed  Google Scholar 

  77. Dou Z, Ghosh K, Vizioli MG, Zhu J, Sen P, Wangensteen KJ, Simithy J, Lan Y, Lin Y, Zhou Z, Capell BC, Xu C, Xu M, et al. Cytoplasmic chromatin triggers inflammation in senescence and cancer. Nature. 2017;550:402–6. https://doi.org/10.1038/nature24050.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  78. van Ruiten MS, Rowland BD. SMC Complexes: universal DNA looping machines with distinct regulators. Trends Genet. 2018;34:477–87. https://doi.org/10.1016/j.tig.2018.03.003.

    Article  CAS  PubMed  Google Scholar 

  79. Hassler M. Shaltiel IA, Haering CH. (2018) Towards a unified model of SMC complex function. Curr Biol 28:R1266-81. https://doi.org/10.1016/j.cub.2018.08.034.

    Article  CAS  Google Scholar 

  80. Marko JF. De Los Rios P, Barducci A, Gruber S. (2019) DNA-segment-capture model for loop extrusion by structural maintenance of chromosome (SMC) protein complexes. Nucleic Acids Res 47:6956–72. https://doi.org/10.1093/nar/gkz497.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Funding from the National Natural Science Foundation of China (31,570,934) and from Foundation of Jilin Educational Committee (project No. JJKH20170380KJ).

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  1. School of Life Sciences, Jilin Normal University, 136000, Siping, People’s Republic of China

    Hongzhen Wang & Xin Liu

  2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, School of Life Sciences, Jilin University, 130012, Changchun, People’s Republic of China

    Hongzhen Wang & Guiying Li

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HZW wrote the manuscript. XL collected some references and revised the manuscript, GYL revised the manuscript. All authors read and approved the final manuscript.

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Wang, H., Liu, X. & Li, G. Explore a novel function of human condensins in cellular senescence. Cell Biosci 10, 147 (2020). https://doi.org/10.1186/s13578-020-00512-1

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Keywords

  • Human condensins
  • Cellular senescence
  • Oncogene-induced senescence
  • Replicative senescence

Cell & Bioscience

ISSN: 2045-3701

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