Table 1 List of primers used in qRT-PCR
Gene | Species | Forward (5ʹ-3ʹ) | Reverse (5ʹ-3ʹ) |
|---|---|---|---|
Renin | Mouse | GCACCGCTACCTTTGAACGA | CCACGGGGGAGGTAAGATTG |
GAPDH | Mouse | GGAGAGTGTTTCCTCGTCCC | ACTGTGCCGTTGAATTTGCC |
Piezo1 | Mouse | GTCATGGACTGGGTGTGGAC | TGGGCTGGGGGTATTTCTTC |
Piezo2 | Mouse | ACTCGTCTGCATCCTACACC | ATTGACTTTGGCATGGCTGT |
TRPM1 | Mouse | CCTGGCCTGAAGGTCATCAT | CTGCATTTGCATCCACATTCTCT |
TRPM2 | Mouse | AAGCCTCTCGAAGCCAAGAC | CAGATCTGCAGGCTTCCACT |
TRPM3 | Mouse | CGAGGACAGCGCAGAGAG | CAGGATTTCTGAGCCGGTGT |
TRPM4 | Mouse | CTTTGGGGCAGCCGTAGTT | TCAGACAGCCGGAGAAAGTTG |
TRPM5 | mouse | ATCACGAGCAACAGCCCTGAG | TTGCATGGTGGCTTTCCAGTC |
TRPM6 | Mouse | TCACCACCATTATTCAGCCATTG | GATGGTCACTTCCTCTCCTGTG |
TRPM7 | Mouse | GCAGCTTTGTTACCGGATTGG | ACGGGCTTAAATGGAGAAGCA |
TRPM8 | Mouse | GGTCATTTGGGAGCAGACCA | TTGGCCAGTTCCTCCGATTC |
TRPV1 | Mouse | AGCGAGTTCAAAGACCCAGAG | TCTGTCTTCCGGGCAATGTC |
TRPV2 | Mouse | TGGGCGTCAGTGTTTTAGGG | GAGGAAGTTCTGGGGTGGTG |
TRPV3 | Mouse | GATTTGGAGTAGCGCTGGC | CAGGCCTATGGTGAGCTTGA |
TRPV4 | Mouse | CATTAACGAGGACCCTGGCA | CCGAGGACCAACGATCCCTA |
TRPV5 | Mouse | GCAAGAAGACATGGGGGCTA | AGTGGAGACTCCCAAATACTTTT |
TRPV6 | Mouse | CTGGAGAGCACAGGTTGTGG | CCAAGACCATACTCTCGCCC |
TRPC1 | Mouse | CGTGCGACAAGGGTGACTAT | CTCCCAAGCACATCTACGCA |
TRPC2 | Mouse | GGACCCCCTTTCGCCACA | CGAGGCATCGGAACTGGATTC |
TRPC3 | Mouse | TGGGTAACTCAAAGTCCAGGTTAAA | TGAGAATGCTGTTAAAACTGTGTGA |
TRPC4 | Mouse | GAAGAGCCAGAGCGAAGAAGAA | ACCCCGTGAAGCTAATCCTTT |
TRPC5 | Mouse | GGCACCTTACCACCTCCTTT | AGCATGACGTTCTGTGAAGC |
TRPC6 | Mouse | CTCTGGTTTACGGCAGCAGA | CGCAATATCTTCCCCATCTTGC |
TRPC7 | Mouse | CTCTCAGGCTTACGGCAACA | AGGGTTTGTCCTAGCTTGCTG |
Ptgs2 | Mouse | GCTCAGCCAGGCAGCAAATC | ACCATAGAATCCAGTCCGGGT |