Fig. 1

Embryoids generation and characterization from hiPSCs of 45,XO, 46,XX, and 46,XY. a Schematic generation of embryoids from hiPSCs via iMeLCs by high-density induction. b The images of hiPSCs (DIC, bar, 100 μm), iMeLCs (DIC, bar, 100 μm), and embryoids (bright field, bar, 200 μm). c Immunofluorescence of SOX17 (green) and TFAP2C (red) expression in day 6 embryoids. Inner sets with white line were enlarged. Images were captured with confocal microscopy. The nuclei were counterstained with DAPI (blue). Bar, 25 μm. d Expression of marker genes of pluripotency, hPGC (early), and hPGC (late). Y-axis indicates expression levels (Log₂ (FPKM + 1))