Fig. 6

Comparison of the testicular transcriptomes of Grsf1^−/− mice and wildtype controls. Total RNA was extracted from testes of Grsf1^−/− mice (n = 3) and wildtype controls [C57Bl/6, n = 4)]. The transcriptomes were quantified as described in the Materials and Methods section. A 287 transcripts including Grsf1 were downregulated (blue), 276 transcripts were upregulated in Grsf1^−/− mice (red) whereas 33,100 transcripts were not significantly different between the two genotypes (blue). B Same as A, with y-axis truncated at − log10(10e−7). Transcripts with an absolute log2 fold-change of > 1.5 and an unadjusted p < 0.05 are shown in red. Transcripts with an absolute log2 fold-change < 1.5and an unadjusted p < 0.05 are depicted in blue. C Overrepresentation analysis was performed as described in the Materials and Methods sections and gene ontology categories are shown. All 563 differentially expressed genes (based on unadjusted p-value of < 0.05) were used as input gene list (Additional file 1: Table S1) and the gene background/universe consisted of 33,663 genes from RNAseq analysis (Additional file 2: Table S2). The top five GO terms per category based on their gene ratio (k/n with k = genes of this cluster belonging to GO term and n = genes of all clusters belonging to GO term) are shown. Biological processes (BP) are depicted in yellow, cellular component (CC) in grey and molecular function in dark blue. D Hierarchically clustered heatmaps of regulated transcripts (unadjusted p < 0.05) of steroid metabolic process. E Hierarchically clustered heatmaps of regulated transcripts (unadjusted p < 0.05) of fatty acid metabolic process. Heatmap colours indicate z-score scaled gene expression values, with blue for low and red for high expression levels