Table 1 Summary table of immunohistochemical markers used in this study
Primary antibody | Marker | Dilution | Species | Provider | Secondary antibody | Incubation time |
|---|---|---|---|---|---|---|
BrDU | Proliferating cells | 1/400 | Sheep | Abcam (#ab1893) | DAS-633 | O/N at 4 °C |
Calbindin | Purkinje cells | 1/1000 | Mouse | Sigma-Aldrich (#C9848) | DAM-594 | O/N (or 72 h with Gluδ2 and Vglut2) at 4 °C |
Cleaved-caspase-3 | Apoptotic cells | 1/400 | Rabbit | Cell Signaling Technology (#9661S) | DAR-488 | O/N at 4 °C |
Gluδ2 | Parallel fibers | 1/500 | Goat | Santa Cruz (#sc26118) | DAG-488 | 72 h at 4 °C |
MBP | Myelinated fibers | 1/300 | Rabbit | Sigma-Aldrich (#M3821) | DAR-488 | O/N at 4 °C |
Vglut2 | Climbing fibers + Mossy fibers | 1/500 | Guinea pig | Millipore (#AB2251) | DAGp-Cy3 | 72 h at 4 °C |