Fig. 3

Alternative splicing analysis of transcripts identified in Mock and TSA treated ovules. In the six libraries including mock and TSA treated, alternative splicing of the transcripts was analyzed. In total, 12 kinds of AS events were defined as follows, and the results showed that TSS and TSS are the dominant AS events identified. (1) TSS: Alternative 5’first exon (transcription start site); (2) TTS: Alternative 3’ last exon (transcription terminal site); (3) SKIP: Skipped exon (SKIP_ON, SKIP_OFF pair); (4) XSKIP: Approximate SKIP (XSKIP_ON, XSKIP_OFF pair); (5) MSKIP: Multi-exon SKIP (MSKIP_ON, MSKIP_OFF pair); (6) XMSKIP: Approximate MSKIP (XMSKIP_ON, XMSKIP_OFF pair); (7) IR: Intron retention (IR_ON, IR_OFF pair); (8) XIR: Approximate IR (XIR_ON, XIR_OFF pair); (9) MIR: Multi-IR (MIR_ON, MIR_OFF pair); (10) XMIR: Approximate MIR (XMIR_ON, XMIR_OFF pair); (11) AE: Alternative exon ends (5′, 3′, or both); (12) XAE: Approximate AE. X: exact boundary match for SKIP, approximate (simple exon overlap) for XSKIP; An exon skipping event as a pair between an exon containing (‘on’) splice form and an exon-skipping (‘off’) splice