Fig. 6

CD44 and αvβ3 were indispensable for SPP1 mediated activation of PKCα and its downstream pathway. a Real-time qPCR determined the expression of PKCα in SCs transfected with si-CD44, si-β3, or si-Control combined with or without rOPN (50 nM) medium. b–f Western blot analysis of PKCα and its downstream cytokines expression levels after siRNA transfection and rOPN treatment in SCs. GAPDH was used as a loading control. b Representative blot. c–f Quantification of the relative expression of proteins. Data were obtained from three independent experiments and presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001