Fig. 2

Calcium deposit levels and ALP activity after osteogenic differentiation of AM-MSCs, UC-MSCs, CM-MSCs, and DC-MSCs. a Calcium deposit levels and b ALP activity were measured spectrophotometrically at days 0 and 21. n = 3/group, Error bars represent the SD, *p < 0.05, **P ≤ 0.01; ***p ≤ 0.001 in different groups compared with the control group by the One-way ANOVA test. c Western blot analysis of osteoblast markers (osterix, collagen I, osteopontin, and osteocalcin) proteins in AM-MSCs, UC-MSCs, CM-MSCs, and DC-MSCs with and without 21 days of osteogenic differentiation. d The relative gray value of osterix, collagen I, osteopontin, and osteocalcin/tubulin