Induction of autophagy protects against cholestatic injury in vivo . Wild-type C57BL/6 mice were pretreated with Rapamycin or CQ intraperitoneally two or three times one week for two weeks, which was followed by BDL induction. Two weeks later, mice were sacrificed and were divided into seven groups: Control, CQ, BDL, CQ + BDL, Rap, BDL + DMSO, Rap + BDL. (A) Blood ALT and AST levels were examined and quantified in each group.(*: P < 0.05). (B) Apoptosis of different groups were determined by TUNEL assay and visualized by microscope. (C) The number of apoptosis (mean ± SD) was quantified from each group. More than 30 cells were counted in each experiment. (*: P < 0.05). (D) Representive photographs of H&E staining were presented. Original magnification: ×200.