Fig. 2
The affinity of mAbs determined by SE. A Real-time interaction of mAbs with covalently immobilised VLPs, n – refractive index of the formed layer. n values as time functions are represented in the graphs. 11D2, 12F8, 4E12 and 5H10 refer to mAb clones. B Schematic representation of IC formation steps, VLPs referred as Ag antigen. C Schematic representation of VLP (antigen) binding to mAbs of various affinities. The affinities of mAbs were expressed in KD and are represented for each mAb. KD values of IgG2a mAbs are indicated in bold. Kinetic parameters—rate constants: ka association, kd dissociation, kr stable IC formation; KA equilibrium association constant, and KDdissociation constant. B and C were created with BioRender.com, AG No.: OZ268OSOXN and AG No.: XD268OT4QA