Fig. 3

Increased expression of C1q in microglia of mouse hippocampus after neonatal sevoflurane exposures. A Volcano plot showing differentially expressed genes (ǀlog₂FCǀ > 1, and FDR < 0.05), including C1qa and C1qb. B Representative confocal images of C1q puncta (green) and nuclei (blue) in the CA1 region. Scale bars = 100 μm. C Quantification showed that sevoflurane increased the expression of C1q. n = 6. Unpaired t-test. D Representative Western blot bands of C1qa in the CA1 region. E Western blot analysis showed that sevoflurane increased the level of C1qa. n = 4. Unpaired t-test. F Representative confocal images of C1q (green) and PSD95 (red) in the CA1 region. Scale bars = 5 μm. G Colocalization analysis revealed that C1q colocalized with PSD95 was elevated in sevoflurane-treated mice. n = 6. Unpaired t-test. H Representative confocal images of C1q (gray)-labeled PSD95 (green) within microglia (red). Scale bars = 5 μm. I Colocalization analysis showed that sevoflurane led to increased colocalization of C1q, PSD95, and IBA1. n = 6. Unpaired t-test. Data are mean ± SEM. *P < 0.05, ** P < 0.01, *** P < 0.001