Fig. 2From: Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradationKowndown of PSMD7 expression restrains the PC cell proliferative capacity in vitro and in vivo. (A) The mRNA of PSMD7 in AsPC-1 and PANC-1 cells transfected with shPSMD7 or shNC assayed via qRT-PCR. (B) PSMD7 knockdown in AsPC-1 and PANC-1 cells was validated via western blotting. (C) The proliferative abilities of AsPC-1 and PANC-1 PC cells with shNC or shPSMD7 treatment was detected separately via CCK-8. (D) Proliferative capacities of AsPC-1 and PANC-1 cells that were transfected with shPSMD7 or shNC were assayed with EdU, and the scale bar represents 50 μm, **p < 0.01. (E) Evaluation of mice that were injected with luciferase-expressing PANC-1/shPSMD7 or PANC-1/shNC cells via the IVIS imaging system. (F) Tumour volumes of the PANC-1/shPSMD7 and PANC-1/shNC groups. Volumes of tumours are expressed as the mean ± SD, n = 6. (G) Tumour weight of the shPSMD7 and shNC groups. n = 6. (H) The images of tumors the PANC-1/shPSMD7 and PANC-1/shNC groups. (I) and (J) Immunohistochemistry (IHC) analysis of PSMD7 and Ki-67 expression in tumours of the PANC-1/shPSMD7 and PANC-1/shNC groups, scale bar denotes 100 μm. n = 3, *p < 0.05, **p < 0.01Back to article page