Synergistic enhancement of the mouse Pramex1 and Pramel1 in repressing retinoic acid (RA) signaling during gametogenesis

Table 2 Primer sequence used for genotyping and RT-PCR

Gene name	Accession number	Primer sequence (5' to 3')	Product length (bp)	Annealing Tm (^oC)
Pramel1	NM_031377	TGGGCTATGTCCATGTATTACCA	185 (WT)/135 (KO)	64
Pramel1	NM_031377	GTCATCGAGAAGGTCTGCCA	185 (WT)/135 (KO)	64
Pramex1	NM_029459	CCAATTCCCCACCTTTTCTT	2270 (WT)/362 (KO)	60
		ACAGCCTGAACCTTGGAGAT	2270 (WT)/362 (KO)	60
		CCAATTCCCCACCTTTTCTT	344 (WT)/236 (KO)	60
		TCCTGAAGGCTAAGCCATGT	344 (WT)/236 (KO)	60
Actb	NM_007393	GAGAAGCTGTGCTATGTTGCT	72 (WT&KO)	64
Actb	NM_007393	CTCCAGGGAGGAAGAGGATG	72 (WT&KO)	64

ISSN: 2045-3701