Fig. 8

The Pramex1/Pramel1 dKO females exhibit reduced number of follicles, which leads to embryo loss. A Representative images of IFS with DDX4 (green) in ovary cross-sections for WT and Pramex1/Pramel1 dKO mice at P3 (n = 3). Bar = 50 µm. B Total number of follicles per ovary. The whole ovary was sectioned, and the counting was performed every five sections, and the number follicles was multiplied by five to determine the total number of follicles per ovary. C–E Results from in vitro fertilization (IVF) experiment. The quality of oocyte with genotyping of "Pramex1⁺, Pramel1⁻" and "Pramex1⁻, Pramel1⁻" were detected through in-vitro fertilize with "Pramex1⁻, Pramel1⁻" or "Pramel1⁻,Y" spermatozoa. C Representative figures of embryos (2-cell and 8-cell) after in vitro fertilization for Pramel1 sKO and Pramex1/Pramel1 dKO oocyte and dKO spermatozoa. Bar = 100 µm. D Number of denuded oocytes and cumulus-oocyte complexes (COCs) in each animal for Pramel1 sKO and Pramex1/Pramel1 dKO (n = 3). E Rate of 2-cell and 8-cell embryos from Pramel1 sKO and Pramex1/Pramel1 dKO oocytes. The rate was calculated by dividing the number of two-cells or eight-cells embryos by the number of COCs. F Representative images illustrating the uteri at E6.5 in WT and dKO mice, aimed at enumerating implantation sites. Bar = 2 mm. G The number of implantation sites in WT and dKO female at E6.5. Data were expressed as mean ± SEM. ** indicates statistically significant difference (P < 0.01)