Fig. 5

Reduced number of undifferentiated spermatogonia in the Pramex1/Pramel1 dKO mice. A Representative images of IFS with ID4 (red) in testis cross-sections for WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice at P35 and P120 (n = 3). Bar = 20 µm. B Number of ID4 + spermatogonia/mm² based on IFS result for the four groups at P35 and P120. The referenced number of ID4 + undifferentiated spermatogonia in the dKO was drawn as red solid line. The IFS experiments in A-B were replicated three times. Significance was assessed among the four samples (WT, Pramex1 sKO, Pramel1 sKO, and dKO mice) at two ages (P35 or P120). Data were expressed as mean ± SEM. Values that do not share a common superscript (a–d) were found to differ significantly (P < 0.05)