Fig. 3

Germ cell apoptosis was increased in the sKO and dKO testis. Testis was evaluated at P7, P14, P21, P28 and P35 and apoptosis was assessed by TUNEL assay in the WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice. A Representative TUNEL (green) staining of testis sections in the WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice at P7 to P15. More apoptotic cells occur in the lumen of seminiferous tubules in the dKO than sKO and WT mice. Bar = 20 µm. B Number of TUNEL-positive (TUNEL⁺) cells in the mouse seminiferous tubules for the four groups of mice. C Percentage of seminiferous tubules with apoptotic cells (TUNEL⁺). Apoptotic cells were significantly increased in the dKO mice than that sum of Pramex1 sKO and Pramel1 sKO. Data were expressed as mean ± SEM