Fig. 2

Deletion of PRAMEL1 and PRAMEX1 in the Pramex1/Pramel1 dKO mice. A Western blot (WB) was used to detect the PRAMEL1 and PRAMEX1 expression in the WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice at P7. B Relative expressions of PRAMEL1 and PRAMEX1 in the Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice to WT (WT was set as 1) were detected in WB. C Cycle threshold (CT) value of qRT-PCR for Pramel1, Pramex1 and Actb genes was compared between the WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice. CT values above 37 indicate minimal amounts or none of target nucleic acid. D Relative expression of Pramel1 and Pramex1 in the Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice to WT (WT was set as 1) were detected by qRT-PCR. PRAMEL1 was not deleted in Pramel1 sKO and Pramex1/Pramel1 dKO mice and ablation of PRAMEX1 in Pramex1 sKO and Pramex1/Pramel1 dKO mice. In addition, higher expression of PRAMEL1 and PRAMEX1 was observed in Pramex1 sKO and Pramel1 sKO, respectively. Significance was assessed among the four groups (WT, Pramex1 sKO, Pramel1 sKO, and Pramex1/Pramel1 dKO mice) for each antibody (anti-PRAMEL1 and anti-PRAMEX1). Data were expressed as mean ± SEM. Values that do not share a common superscript (a–c) were found to differ significantly (P < 0.05)