Fig. 5
From: CRISPR/Cas9 mediated Y-chromosome elimination affects human cells transcriptome
Validation of down- (A) and up- (B) regulated genes resulted from the RNA-seq analysis. RT-PCR analyses were performed in triplicate by using RNA extracted from ARPE-19 Y-depleted clones 3, 5 and 9 VS ARPE-19 WT. Each bar represents mean ± SD calculated from three independent experiments. P values were derived from Student’s t test between the indicated samples (ns: P > 0.05; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001)