Fig. 1

WRN deficiency accelerates adipocyte metabolism. a–h qRT-PCR analysis of selected adipogenic markers PPARγ (a), CEBPα (b), UCP1 (c), PGC (d), FABP4 (e), ADIPQ (f), ELOVL6 (g), and ACACA (h) in hMSCs (N = 3 biological replicates). i–k Representative immunofluorescent images of PPARγ (i), CEBPα (j), and ADD1 (k) (N = 3 biological replicates). Scale bar = 20 μm. l–o Representative Oil Red O staining images during adipogenesis (N = 3 biological replicates). p Illustration of white and brown adipocytes differentiation following previous report [25]. q, r qRT-PCR analysis of selected white adipogenic markers PPARγ (q) and CEBPα (r) in hESCs (N = 3 biological replicates). s, t qRT-PCR analysis of selected brown adipogenic markers CIDEA (s), and UCP1 (t) in hESCs (N = 3 biological replicates). Data are presented as the mean ± S.D. Statistical analysis was performed using two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001