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Fig. 7 | Cell & Bioscience

Fig. 7

From: mTOR hypoactivity leads to trophectoderm cell failure by enhancing lysosomal activation and disrupting the cytoskeleton in preimplantation embryo

Fig. 7

mTOR inhibition in preimplantation embryo disrupt cytoskeletal organization, and lead to blastomeres apoptosis. A FITC-phalloidin and E-cadherin immunofluorescence analysis to detect F-actin (green) and E-cadherin (red) in control (Con), rapamycin (RPY), JR-AB2-011 (JR) and Raplink-1 (RPL) treated embryos at 8-cell stages for 24 h development. Bar, 15 μm. B Immunofluorescence analysis to detect F-actin (green) and TUNEL staining to detect apoptosis in the blastocysts of control and/or rapalink1-treated groups. Nuclei (blue) are stained with DAPI. Bar, 15 μm. C GSEA profiles showing a significant enrichment of gene ontology associate with negative regulation of epithelial cell apoptotic process in RPL and control blastocysts (RPL vs Con). D The heatmap shows the expression pattern of apoptosis-related genes in RPL and Con embryos

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Cell & Bioscience

ISSN: 2045-3701

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