Fig. 6

Combined analysis of DNA methylation pattern and transcriptional expression of blastocysts derived from mTOR inhibitor treatment during 8-cell to blastocyst development. A The morphology of blastocysts from the control (Con) and rapalink-1 (RPL) groups that were used for genome-wide bisulfite sequencing. B Heatmap showing the level of DNA methylation of the CpG site in each group of blastocysts. C The levels of genome-wide DNA methylation in RPL and Con are shown (n = 3). D The number and proportion of hypermethylated and hypomethylated differentially methylated regions (DMRs) in RPL vs Con. E Venn diagram of DMRs-associated genes (DMGs) and differentially expressed genes (DEGs) detected by pair-wise comparison. F Bar chart illustrating the number and proportion of hypermethylated-downregulated genes (Hyper-down), hypermethylated-upregulated genes (Hyper-up), hypomethylated-downregulated genes (Hypo-down) and hypomethylated-upregulated genes (Hypo-up) from overlapped genes in DMGs and DEGs. G Bar chart illustrating the enriched top GO terms associated with the overlapped genes of hyper-down. H Bar chart illustrating the enriched top GO terms associated with the overlapped genes of hyper-up. I Bar chart illustrating the top 10 enriched KEGG terms or canonical pathways in overlapped genes of hyper-down. J Bar chart illustrating the top 10 enriched KEGG terms or canonical pathways in overlapped genes of hyper-up