Fig. 1

Developmental phenotypes of embryos from pregnancy mouse that were inhibition of mTOR signaling during preimplantation development. A Schematic illustration of the experimental setup of mouse treatment, embryo recovery and detection. B Immunostaining showing the levels of pS6 and p-AKT after 24 h of treatment with mTOR inhibitors at the 1-cell stage. Scale bars, 20 μm. C Representative bright-field images of embryos recovered from corn oil and RPL treated mice at 3.5 dpc. Scale bars, 100 μm. D The percentage of blastocysts was calculated at 3.5 dpc (n = 3). E Assessment of the implantation potential of blastocysts derived from corn oil and RPL treated mice (n = 12). F HE stained sections of paraffin-embedded embryos at 5.5 dpc. em, embryo. de, decidua. G Calculation of the number of fetuses recovered from the uteri at 13.5 dpc (n = 11). H Representative images showing conceptuses at 13.5 dpc and quantification of embryo crown-rump length (n = 43 for Corn oil and 64 for RPL). Production of offspring (I) (n = 10 for Corn oil and 12 for RPL) and the body weights (J) of offspring (n = 127 for Corn oil and 131 for RPL) from corn oil and RPL treated embryos. Error bars are mean ± SEM. ** p < 0.01, * p < 0.05, n.s., not significant (p > 0.05). RPL Rapalink-1, dpc days postcoitus, HE Hematoxylin and eosin