Table 6 Potential biomarkers for liquid biopsy in breast cancer bone metastases
Biomarkers | Category | Techniques used | Refs. |
|---|---|---|---|
RANKL | Protein | ELISA | [88] |
SCUBE2 | Protein | ELISA | [28] |
P1NP, CTX, 1-CTP | Protein | Immunological analysis | [96] |
uNTx | Protein | ELISA | [147] |
IBSP Exosomal miRNA-19a | RNA, protein | ELISA (IBSP) RT-qPCR (Exosomal miRNA-19a) | [98] |
miRNA-30b-5p | RNA | RT-qPCR | [99] |
miR-378 and miR-16 | RNA | RT-qPCR | [102] |
HSP90AA1, SPP1, IL3, VEGFA, PTK2 | Gene panel | RT-qPCR | [120] |
CTC | Cell | Immunomagnetic sorting | |
cOC | Cell | Flow cytometry and immunofluorescence staining | [140] |