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Fig. 3 | Cell & Bioscience

Fig. 3

From: Captive ERVWE1 triggers impairment of 5-HT neuronal plasticity in the first-episode schizophrenia by post-transcriptional activation of HTR1B in ALKBH5-m6A dependent epigenetic mechanisms

Fig. 3

ERVWE1 upregulated HTR1B leading to ERK-ELK1-Arc signaling pathway activation. A Representative western blot result of HTR1B in SH-SY5Y cells. B ERVWE1 increased p-ERK1/2, p-ELK1, and Arc protein levels in SH-SY5Y cells. C The relative protein levels of ERK1/2, p-ERK1/2, ELK1, p-ELK1, and Arc when co-transfected with ERVWE1 (or empty plasmid) and siHTR1B (or siNC) in SH-SY5Y cells. D The diagrammatic drawing for 5-HT neurons isolated from the DRN of newborn Sprague–Dawley Rats (P0–P7). E TPH2 immunostaining showed the percentage of 5-HT neurons in DRN neurons (n = 5 rats per group). F, G Western blot results of HTR1B and downstream proteins expression levels after transfection with ERVWE1 in DRN neurons. H, I Immunofluorescence staining with Arc in DRN neurons. J DRN neurons were transfected with ERVWE1 and siHTR1B. Data are mean ± SD. Statistical analysis: Student’s t-test and one-way ANOVA (ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). All experiments were repeated 3 times

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Cell & Bioscience

ISSN: 2045-3701

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