Fig. 4

Inhibition of NK1-mediated NK2/β-arrestin1 interaction by NK1-specific inhibitor. A The effect of aprepitant on 1 μM SP-stimulated β-arrestin1 recruitment to NKs was examined in cells expressing NanoBiT constructs of NKs and β-arrestin1 with or without NK2. Cells were pretreated with 1 μM aprepitant for 30 min before the addition of SP. B Aprepitant inhibited 10 nM SP-stimulated interaction of NK2-LgBiT and β-arrestin1-SmBiT in the presence of NK1. C Aprepitant inhibited NKA-stimulated β-arrestin1 recruitment to NK1 but not NK2. Cells expressing NanoBiT constructs of NKs and β-arrestin1 with or without another NKs were pretreated with aprepitant for 30 min and then stimulated with 10 nM NKA. Luciferase activities were measured with a luminometer