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Fig. 2 | Cell & Bioscience

Fig. 2

From: Neurokinin-2 receptor negatively modulates substance P responses by forming complex with Neurokinin-1 receptor

Fig. 2

Effect of NK1 on SP-stimulated β-arrestin1 recruitment to NK2. A Cells expressing NK2-LgBiT and β-arrestin1-SmBiT with intact NKs were treated with different concentrations of SP and changes of luciferase activities were measured in real time. Maximal activities from each concentration were plotted on the graph. B Time-dependent responses at designated concentration which was boxed area in graphs of (A). C Basal luciferase activities in cells expressing NanoBiT constructs with intact receptors. D Effect of NKs on SP-stimulated β-arrestin1 recruitment to NK1. Upper graph: dose-dependent curves of SP-stimulated β-arrestin1 recruitment to NKs. Lower graph: luciferase activities of NK1-SmBiT and β-arrestin1-LgBiT in the presence of intact NKs in cells treated with 0.1 nM SP, a concentration that stimulated NK1 but not NK2 (indicated by red arrow in the upper graph)

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Cell & Bioscience

ISSN: 2045-3701

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