Fig. 6

Asc regulates glycolysis via a DKG-independent route. A The effects of AscPNa, DHAA, and DKG on key components in glycolysis were analyzed. 2PG: 2-phosphoglycerate; 3PG: 3-phosphoglycerate; G1P: glucose-1-phosphate; G6P: glucose-6-phosphate; F6P: fructose-6-phosphate. B–D The abilities of the two types of Asc treatment (0–8 h and 8–24 h) to affect ECAR B, OCR C, and cell migration D were determined. E–G Similar experiments were performed on day 3 during reprogramming as in B-D. Metabolome analysis was performed at least eight times (n ≥ 8), while other experiments were repeated at least five times (n ≥ 5). Additional statistical information is listed in Additional file 4: Table S3.