Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair

Cell & Bioscience

Table 1 KI efficiency in pig parthenogenetic embryos treated with small molecules

Treatment	Injected embryos	2-cell (%)	Blastocysts (%)	KI rate (%)
Control	466	77.52 ± 3.06^ab	48.87 ± 4.84^c	11.81 ± 1.84^a
Irinotecan (5 µM)	460	81.12 ± 2.46^b	46.97 ± 4.62^c	23.61 ± 2.50^b
Docetaxel (0.5 µM)	460	62.97 ± 3.36^ab	19.14 ± 3.46^b	23.61 ± 3.67^b
Mitomycin C (0.5 µM)	447	70.81 ± 4.98^ab	18.31 ± 4.66^b	22.22 ± 2.78^b
Nocodazole (0.1 µM)	302	75.53 ± 3.48^ab	42.00 ± 4.59^c	30.56 ± 1.39^C
Irinotecan + Mitomycin C	120	70.83 ± 5.83^ab	5.00 ± 1.67^a	25.00 ± 25.00^abc
Docetaxel + Nocodazole	184	56.14 ± 8.07^a	16.14 ± 2.66^b	30.56 ± 2.78^C

KI rates are ratios of blastocysts with HindIII-cut alleles to all blastocysts
^a,b,cDifferent letters indicate statistically significant differences between groups (mean ± SEM, P < 0.05)

ISSN: 2045-3701