Fig. 6
From: Modulation of cell cycle increases CRISPR-mediated homology-directed DNA repair
Overexpression of cell cycle factors increases KI frequency. A Overexpression of CCNA2, CCNB1 and CDK1 in 293T cells by transiently transfection of the eukaryotic expression vectors harboring cDNA of the genes. Enhanced KI frequency tested by further transfection of GAPDH-EGFP reporter shown in Additional file 3: Fig. S1 in overexpressing cells. B Overexpressing all tested genes in 293T cells showing promoting effect on KI, representing by increased proportion of EGFP-positive cells in CCNA2, CCNB1 and CDK1-transfected cells compared to mock transfection control. Data are mean ± SD of nine independent experiments. **P < 0·01 vs mock transfection control