Fig. 4

RNAseq analysis of 293T cells with cell cycle arrest with small molecules. A Gene Ontology (GO) analysis on RNAseq data showing significantly enriched GO terms in cell cycle, DNA repair and mitosis upon small molecule treatment. The differentially enriched GO terms implying the different action mechanism for cell cycle synchronization among the small molecules. B PCA showing IRI and MITO treatment groups clustered farther from the control than DOC and NOC groups, indicating the more profound change in transcriptome profile of cells with IRI and MITO treatment. C Cell cycle-associated genes, CCNA2, CCNB1/2, CDK1 and CDK2, are significantly upregulated in IRI and MITO treatment groups, whereas only CCNB1 had significant increase in mRNA level in DOC and NOC treatment groups. D CDK4 and CDK6 controlling G1 cell cycle phase showing insignificant difference or decrease in mRNA level of cells with small molecule treatment. E NHEJ factors (PRKDC, KU70 and KU80) and HDR factor (RAD51) showing decreased mRNA level in most groups with small molecule treatment. Gene expression levels were represented by normalized FPKM values. Data are mean ± SD from 3 independent samples. **P < 0·01 and *P < 0·05 vs DMSO-treated control