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Table 3 Cell transformation state under different culture conditions

From: A rapid and stable spontaneous reprogramming system of Spermatogonial stem cells to Pluripotent State

Conditions

transformation state

LIF

EGF

MEF

FBS

siRNA

+

+

+

+

/

Y

+

/

+

+

/

N

/

+

+

+

/

N

+

+

+

/

/

N

+

+

/

+

/

N

+

+

+

+

Kras

N

+

+

+

+

Nras

N

+

+

+

+

Hras

N

+

+

+

+

+

+

+

+

+

+

+

+

Rac1

Snail

Smad3

N

N

N

  1. +: included; /: not included; Y: transformed colonies were observed within 10 passages; N: transformed colonies were not observed within 10 passages. n ≥ 3