Table 1 Comparison of the transformation time and efficiency

Primary testicular cells

appear in 1-2 M, dominant in 2-3 M

occasional [7]

SSCs cell line from neonatal testis

5 M

not observed [7]

p53 KO SSCs from neonatal testis

6 M

2 out of some experiments [7]

p53 KO SSCs from mature testis

6 M

50% (4 out of 8 experiments) [7]

SSCs from neonatal testis in med 1

4 M (25 passages)

27.5% (11 out of 40 experiments)

SSCs from neonatal testis in med 1 for 5 passages and changed med 2

1.5 M dominant (10 passages: 5 in med 1, 5 in med 2)

71.4% (10 of 14 experiments)

SSCs from neonatal testis in med 1 for 8 passages were changed med 2

1.8 M dominant (13 passages: 8 in med 1, and 5 in med 2)

85.7% (12 of 14 experiments)

SSCs from neonatal testis in med 1 for 10 passages were changed med 2

dominant in 2 M (15 passages: 10 in med 1, and 5 in med 2)

100% (6 of 6 experiments)