Fig. 7

Long-term cultured SSCs failed to transform on laminin. a-d The morphologies of SSCs after 30 passages grown on MEF feeder (a) were transferred to laminin for 1 passage (b) and another 5 passages (arrowheads indicate some newly formed colonies) (c), and primary SSCs cultured on laminin for 10 passages (d) were exhibited. e-h IF staining was used to detect PLZF (e), NANOG (f), DAPI (g) and merge (h) in SSCs of 30 passages cultured on MEF feeder layer. i-p IF staining was used to detect PLZF (i), NANOG (j), DAPI (i), DAPI (k), merge from i to k (l), OCT4 (m), SOX2 (n), DAPI (o) and merge of m to o (p) in SSCs of 30 passages cultured on MEF feeder and subsequently cultured on laminin for 5 passages (arrows indicate representative cell clusters exhibited by immunofluorescence). Scale bar = 20 μm