Fig. 3

Mettl3 deletion in myeloid cells facilitates the recruitment of Gr-1⁺ MDSCs. (A) Flow cytometry analysis of total PerC cells demonstrated gradual recruitment of CD11b⁺Gr-1⁺ MDSCs during the early and mid-phase ID8 tumorigenesis, and considerably increased MDSCs recruitment in the peritoneum of Mettl3-cKO mice was observed during the late stage of tumorigenesis. (B) MDSCs are further classified into PMN-MDSCs and M-MDSCs based on Ly6G and Ly6C expression, respectively. (C) Multiple cytokines in the peritoneal lavage of mice at weeks 1, 4, and 8 were analyzed with ELISA. Each dot represents one animal. Bars represent SD; *p < 0.05, **p < 0.01, ***p < 0.005