Fig. 2

Mettl3 deficiency in macrophages alters the composition and not polarization of peritoneal SPMs and LPMs. The total PerC cells were collected and analyzed with flow cytometry. (A) Representative FACS plots and percentage of SPMs and LPMs at 1, 4, and 8 weeks indicated a slight increase of SPMs but decreased LPMs in WT and Mettl3-cKO mice throughout tumorigenesis. (B-C) Representative FACS plots of M1 and M2 subsets (based on CD11b expression) at weeks 2, 5, and 9. (D) Representative FACS plots of M2 differentation of ICAM-1⁺ SPM and MHC-II⁺ LPM. The percentage of two macrophage subsets indicated similar M1 and M2 polarization between WT and Mettl3-cKO mice. (E) The percentage of CD4 + T and CD8 + T cells revealed no discrepancy between WT and Mettl3-cKO mice. Each dot represents one animal. Bars represent SD.