Fig. 1
ID8 cell growth in WT and Mettl3-cKO mice. (A) 5 × 105 ID8 cells were intraperitoneally injected into WT and Mettl3-cKO mice. Mice were sacrificed at 1, 4, and 8 weeks. Total PerC cells were obtained for each mouse by pooling ascites (if present) and peritoneal lavage. The percentage of ID8 (live CD45−) cells was analyzed by flow cytometry, and the ID8 cell count was assessed based on the total cell count. Each dot represents one animal. Bars represent SD; *p < 0.05, **p < 0.01, ***p < 0.001. (B) Representative images of mice abdomen indicating severe ascites formation in Mettl3-cKO mice at week 8. (C) The volume of ascites collected from WT and Mettl3-cKO mice at week 8. Each dot represents one animal. Bars represent SD; ***p < 0.001. (D) Representative H&E staining images of total PerC cells indicate that tumor-bearing Mettl3-cKO mice had more and larger spheroids than WT mice. (E) The numbers of spheroids are normalized to each 100 µL ascites from WT and Mettl3-cKO mice at week 8. Each dot represents one animal. Bars represent SD; *p < 0.05. (F) The immunofluorescent staining assay confirms a close interaction between PMs and ID8 tumor cells. PMs: CD68+; ID8 cells: Ki67+. (G) Percentages and total numbers of Ki67+ cells, indicating ID8 tumor cells, were compared between tumor-bearing WT and Mettl3-cKO mice at week 8. Each dot represents a cell count in one field. Bars represent SD; ns, p > 0.05, *p < 0.05