Fig. 4

METTL3/FTO/ALKBH5 regulated the m6A modification of c-myc. A: RIP assay was performed to check the interaction effect between MYC and methylation transferase as well as demethylase; B: RNA pull-down was conducted to detect interaction effects between MYC and METTL3, FTO or ALKBH5; C-D m6A levels of MYC was measured after knockdown of METTL3, FTO, and ALKBH5; E: SRAMP analysis (http://www.cuilab.cn/sramp) was applied to predict m6A modification sites on MYC mRNA; F: schematic diagram of m6A site mutation construct; G-I: dual luciferase reporter vector assay was employed to confirm the modification sites of METTL3/FTO/ALKBH5 on MYC; J: immunofluorescence in situ hybridization was conducted to verify the co-localization of METTL3/FTO/ALKBH5 and MYC in cells Situation. *P < 0.05; **P < 0.01; ***P < 0.001