Fig. 6
From: A SNARE protective pool antagonizes APOL1 renal toxicity in Drosophila nephrocytes
SNARE proteins reduce APOL1-induced cell death in human kidney cells, with efficiency dependent on binding affinity. A HEK 293T cells expressing Flag-tagged APOL1-G0 with HA-tagged dSyx7, dYkt6 or dSyb were lysed, and the extracts were immunoprecipitated using anti-Flag M2 affinity gel, followed by immunoblotting with Flag or HA antibody. IB, immunoblotting. B HEK 293T cells expressing the indicated Flag-tagged APOL1 variant with HA-tagged dSyx7 were lysed, and the extracts were immunoprecipitated using anti-Flag M2 affinity gel, followed by immunoblotting with Flag antibody. C Quantification of the experiments represented in B. Data represented as mean ± SD; n = 3 independent experiments. Data represented as mean ± SD. Statistical significance (*) P < 0.05, (***) P < 0.0005